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Rio de Janeiro, Brazil

Pacheco-Rivera R.A.,Laboratorio Of Diagnostico Molecular | Pacheco-Rivera R.A.,Laboratorio Of Oncologia Genomica | Hernandez-Zamora E.,Instituto Nacional Of Rehabilitacion | Gonzalez-Yebra B.,University of Guanajuato | And 5 more authors.
Clinical and Experimental Medicine | Year: 2011

The most important mutation associated with Multiple Endocrine Neoplasia type 2B (MEN 2B) is the change of thymine to cytosine in codon 918 of exon 16 in the RET oncogene (ATG → ACG). The aim of this work was to develop a single oligoarray by using tandem hybridization to detect the T918C/RET mutation for MEN 2B patients. Two genetically non-related families were studied; each family had a member affected by MEN2B. Both patients presented the T918C/RET mutation in a heterozygous fashion. None of the relatives was positive for this mutation; thus, these cases arose de novo. The proper mutation was confirmed by with different tools, PCR-Fok I endonuclease, direct sequencing, and also using our oligoarray. In this case, it is suitable to use a DNA target smaller than 150 bases with single- or doublestranded DNA and short probes of 7-mer. It was also possible to detect the mutation by employing different sources of DNA, fresh or paraffin-embedded tissues. Therefore, the present oligoarray can identify the most common M918T mutation of RET oncogene from a variety of DNA sources with good specificity and be a good alternative in the molecular diagnosis for MEN 2B cases. © Springer-Verlag 2011. Source

MacIas-Vega M.,Laboratorio Of Diagnostico Molecular | MacIas-Vega M.,Instituto Nacional Of Pediatria | Garcia-Flores J.R.,Laboratorio Of Diagnostico Molecular | Miranda-Gonzalez E.,Laboratorio Of Diagnostico Molecular | Paez-Rodriguez J.,Laboratorio Of Diagnostico Molecular
Revista Espanola de Medicina Legal | Year: 2013

Introduction: Nowadays in Mexico the use of short tandem repeats has increased in Forensic practice for individual identification, due to its excellent discriminative power and its widespread use in laboratories around the world. Objective: The aim of this research work was to delve into the knowledge of allelic frequencies from markers such as D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA in the metropolitan area in the central region of Mexico. Material and methods: A study about the allelic frequencies obtained from 300 healthy and unrelated individuals was developed. Data were analyzed statistically. Results and conclusions: The combined power of discrimination was 0.999999999. All loci are in Hardy-Weinberg equilibrium, and show high discrimination for paternity analysis and forensic genetic applications. Results contribute to establishing a representative database of genome admixture in the region, and show the marked genetic heterogeneity that characterizes Latin American populations. © 2012 Asociacón Nacional de Médicos Forenses. Published by Elsevier España, S.L. All rights reserved. Source

Ferreira T.,Federal University of Rio de Janeiro | Ferreira T.,Laboratorio Of Diagnostico Molecular | Farah A.,Federal University of Rio de Janeiro | Oliveira T.C.,Laboratorio Of Diagnostico Molecular | And 3 more authors.
Food Chemistry | Year: 2016

Coffee is one of the main food products commercialized in the world. Its considerable market value among food products makes it susceptible to adulteration, especially with cereals. Therefore, the objective of this study was to develop a method based on Real-Time Polymerase Chain Reaction (PCR) for detection of cereals in commercial ground roast and soluble coffees. After comparison with standard curves obtained by serial dilution of DNA extracted from barley, corn and rice, the method was sensitive and specific to quantify down to 0.6 pg, 14 pg and 16 pg of barley, corn and rice DNA, respectively. To verify the applicability of the method, 30 commercial samples obtained in different countries were evaluated and those classified as gourmets or superior did not present the tested cereals DNA. However, barley was detected in various traditional (cheaper) samples from South America. In addition, corn and rice were also detected in different samples. Real-Time PCR showed to be suitable for detection of food adulterants in commercial ground roast and soluble coffees. © 2015 Elsevier Ltd. Source

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