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Raspagliesi D.,Laboratorio Of Diagnosi E Biotecnologie Fitosanitarie | Licciardello G.,Laboratorio Of Diagnosi E Biotecnologie Fitosanitarie | Lombardo A.,Laboratorio Of Diagnosi E Biotecnologie Fitosanitarie | Catara A.,Laboratorio Of Diagnosi E Biotecnologie Fitosanitarie | Rizza S.,University of Catania
Acta Horticulturae | Year: 2011

Three SY isolates of Citrus tristeza closterovirus (CTV) found in Sicily (SG29, TDV, Tapi) were analysed by Capillary Electrophoresis-Single-Strand Conformation Polymorphism (CE-SSCP) of p18, p23 and p27 genes. The profiles were found to belong to two groups with differences between SG29 and Tapi and TDV. This newly emerging approach has proved to be robust and highly reproducible. The standard deviation means of the migration data for a total of 72 analyses of genes and isolates was very low (±0.046 and ±0.052 for forward and reverse strands, respectively). BLAST homology analysis of p18 and p23 genes confirmed the CE-SSCP groupings from peak positions relative to internal size markers. According to the results, the method appears to be a powerful tool for quickly characterizing the genetic diversity of CTV isolates. It is simple to use, highly sensitive and provides reproducible results which means a large number of samples can be processed at once.

Yahiaoui D.,Instituto Agronomico Mediterraneo Of Bari | Yahiaoui D.,University of Catania | D'Onghia A.M.,Instituto Agronomico Mediterraneo Of Bari | Djelouah K.,Instituto Agronomico Mediterraneo Of Bari | And 2 more authors.
Journal of Plant Pathology | Year: 2015

Citrus tristeza virus (CTV) is the causal agent of devastating epidemics that have changed the course of citrus industry worldwide. Sixty-eight Mediterranean and eight exotic CTV isolates were characterized based on sequence divergence within the 5’ terminal genomic region and the major coat protein gene (CP). Different genotype profiles were assigned to each of the isolates examined according to their specific signals against a set of reaction to selected molecular markers. High sequence polymorphism in the CP region was observed from various CTV isolates between and within countries. Results indicated that T30 mild strain genotype was a stable viral component among the isolates under investigations. CP haplotypes showed high nucleotide identities with standard strains associated with high virulence behavior, such as T3 and SY568 while severe VT and T36 genotypes were the main variants in the Middle East and East Adriatic regions, respectively. The possible rapid diffusion of these severe CTV strains by aphids raises the risk of new outbreaks in the Mediterranean region. © 2015, Edizioni ETS. All rights reserved.

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