Rio de Janeiro, Brazil
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Marques Peixoto F.,Federal Rural University of Rio de Janeiro | Marques Peixoto F.,Fundacao Centro Universitario Estadual da Zona Oeste | Fernandes I.,University of Porto | Gouvea A.C.M.S.,Federal Rural University of Rio de Janeiro | And 6 more authors.
Journal of Functional Foods | Year: 2016

The bioaccessibility of the anthocyanins from peel powder of jabuticaba, jamelão and jambo fruits under gastro-intestinal conditions and their gastric and intestinal absorption was examined in order to estimate anthocyanin transport efficiency (ATE). Thus, in vitro digestion coupled to epithelium absorptive models (gastric MKN-28 and intestinal Caco-2) was applied independently for each phase. The bioaccessibility of anthocyanins after gastric digestion were 13% for jabuticaba, 45% for jambo and 65% for jamelão, whereas intestinal bioaccessibility were 10% for jabuticaba, 15% for jambo and 45% for jamelão. The assays showed that, applying the MKN-28 cell model, the ATE were 19.7, 9.7 and 14.1%, respectively, for jambo, jabuticaba, and jamelão powders, whereas using the Caco-2 cell model these results were 0.8, 0.2 and 0.3%, respectively. In conclusion gastric mucosa plays an important role in anthocyanin's absorption. © 2016 Elsevier Ltd.


Diniz G.S.,Federal University of Fluminense | Diniz G.S.,Federal University of Rio de Janeiro | Barbarino E.,Federal University of Fluminense | Oiano-Neto J.,Laboratorio Of Cromatografia Liquida | And 2 more authors.
Latin American Journal of Aquatic Research | Year: 2014

The chemical profiles of Desmapsamma anchorata, Hymeniacidon heliophila (Porifera), Bunodosoma caissarum, Renilla muelleri (Cnidaria), Aplysia brasiliana, Eledone massyae, Isognomon bicolor (Mollusca), Echinaster brasiliensis, Echinometra lucunter, Holothuria grisea, Lytechinus variegatus (Echinodermata), and Phallusia nigra (Chordata) were determined. Hydrosoluble protein was the most abundant class of substances for all species, except for the ascidian Phallusia nigra, in which the carbohydrate content was higher. The percentages of hydrosoluble protein (dry weight, dw) varied widely among the invertebrates, ranging from 5.88% (R. muelleri) to 47.6% (Eledone massyae) of the dw.The carbohydrate content fluctuated from 1.3% (R. muelleri) to 18.4% (Aplysia brasiliana) of the dw. For most of the species, lipid was the second most abundant class of substances, varying from 2.8% (R. muelleri) to 25.3% (Echinaster brasiliensis) of the dw. Wide variations were also found for the invertebrates nitrogen content, with the lowest value recorded in the cnidarian R. muelleri (2.02% of the dw) and the highest in the molluscan E. massyae (12.7% of the dw). The phosphorus content of the dw varyed from 0.24% (R. muelleri) to 1.16% (E. massyae). The amino acid composition varied largely among the species, but for most of the species glycine, arginine, glutamic acid, and aspartic acid were the most abundant amino acids, with histidine and tyrosine among the less abundant amino acids. The actual content of total protein in the samples was calculated by the sum of amino acid residues, establishing dw values that fluctuated from 11.1% (R. muelleri) to 66.7% (E. massyae). The proteinaceous nitrogen content was high in all species, with an average value of 97.3% of the total nitrogen. From data of total amino acid residues and total nitrogen, specific nitrogen-to-protein conversion factors were calculated for each species. The nitrogen-to-protein conversion factors ranged from 5.10 to 6.15, with an overall average of 5.45. The use of the specific nitrogen-to-protein conversion factors established here is recommended, since it would yield more accurate determinations of total protein in the species tested in this study.


Diniz G.S.,Federal University of Fluminense | Diniz G.S.,Federal University of Rio de Janeiro | Barbarino E.,Federal University of Fluminense | Oiano-Neto J.,Laboratorio Of Cromatografia Liquida | And 2 more authors.
Latin American Journal of Aquatic Research | Year: 2013

The amino acid composition and contents of nitrogen, phosphorus, lipid, carbohydrate and protein were determined in muscles of Dactylopterus volitans, Genypterus brasiliensis, Mullus argentinae, Paralichthys patagonicus, Percophis brasiliensis, Pinguipes brasilianus, Rhizoprionodon lalandii, Rhizoprionodon porosus, and Urophycis cirrata. The samples showed low carbohydrate content (<3.5% dry weight in all species) and were rich in protein (>66% dry weight in all species). The percentage of total lipid varied widely among species, and M. argentinae showed the highest concentrations (16%). The percentage of nitrogen and phosphorus was high and similar among species, with overall average values of 13.3% and 1.2%, respectively. The amino acids composition was similar among the animals, with glutamic acid and lisine as the most abundant amino acid and histidine in low concentrations. Among species, the content of proteinaceous nitrogen was high, with an average of 96.8% of the total nitrogen. From data of total amino acid and total nitrogen, specific nitrogen-to-protein conversion factors were calculated for each species. The nitrogen-to-protein conversion factors ranged from 5.39 to 5.98, with an overall average of 5.71. These findings showed that the traditional conversion factor of 6.25 overestimates the actual protein content and should be avoided.


Da Cunha C.P.,Federal Rural University of Rio de Janeiro | Godoy R.L.D.O.,Laboratorio Of Cromatografia Liquida | Braz-Filho R.,State University of Norte Fluminense | Braz-Filho R.,Federal Rural University of Rio de Janeiro
Revista Virtual de Quimica | Year: 2016

Dipteryx odorata (Aubl.) Willd, Fabaceae family, is a native species from the Amazon forest, Brazil. The goal of the work was to isolate and identify the flavonoids in the endocarp from D. odorata in order to minimize costs with solvents, time and residues. Six flavonoids, 3′,4′,7-trihydroxyflavone, 3′,4′,7-trihydroxyflavanone, 3′,4′,6-trihydroxyaurone, 3′,4′,5,7-tetrahydroxyflavone, 2′,3,4,4′-tetrahydroxychalcone and 2′,4,4′-trihydroxychalcone were isolated. The flavonoids 3′,4′,7-trihydroxyflavoneand 2′,3,4,4′-tetrahydroxychalcone were identified for the first time in this species.


Gouvea A.C.M.S.,Federal Rural University of Rio de Janeiro | Melo A.,University of Porto | Santiago M.C.P.A.,Laboratorio Of Cromatografia Liquida | Peixoto F.M.,Federal Rural University of Rio de Janeiro | And 3 more authors.
Food Chemistry | Year: 2015

Abstract Neomitranthes obscura (DC.) N. Silveira is a Brazilian fruit belonging to the Myrtaceae family that contains anthocyanins in the peel and was studied for the first time in this work. Delphinidin-3-O-galactoside, delphinidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, petunidin-3-O-glucoside, pelargonidin-3-O-glucoside, peonidin-3-O-galactoside, peonidin-3-O-glucoside, cyanidin-3-O-xyloside were separated and identified by LC/DAD/MS and by co-elution with standards. Reliable quantification of anthocyanins in the mature fruits was performed by HPLC/DAD using weighted linear regression model from 0.05 to 50 mg of cyaniding-3-O-glucoside L-1 because it gave better fit quality than least squares linear regression. Good precision and accuracy were obtained. The total anthocyanin content of mature fruits was 263.6 ± 8.2 mg of cyanidin-3-O-glucoside equivalents 100 g-1 fresh weight, which was in the same range found in literature for anthocyanin rich fruits. © 2015 Published by Elsevier Ltd.

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