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Arruda M.S.,Federal University of Pernambuco | Arruda M.S.,Federal Rural University of Pernambuco | Silva F.O.,Federal University of Pernambuco | Silva F.O.,Federal Rural University of Pernambuco | And 7 more authors.
LWT - Food Science and Technology | Year: 2012

The casein and its hydrolysates have many desirable functional properties, so there is great interest in its use in food and pharmaceutical industries. The hydrolysis of casein by a new protease obtained from latex . Jacaratia corumbensis was performed to obtain antimicrobial peptides. Four proteins were visualized by electrophoresis of the enzyme extract of . J. corumbensis, a band of molecular weight of approximately 30 kDa with the presence of other bands of low density. The fractions αs-CN, β-CN and κ-CN, were subjected to hydrolysis for a period of 1 min 24 h, where aliquots were collected and analyzed by SDS-PAGE to the characterization of peptides. The hydrolysates were evaluated for antimicrobial activity by determining the Minimum Inhibitory Concentration (MIC). The hydrolysates obtained at time of 2 h showed antimicrobial activity against microorganisms . Enterococcus faecalis, . Bacillus subtilis, . Escherichia coli, . Pseudomonas aeruginosa, . Klebsiella pneumoniae and . Staphylococcus aureus, indicating that the peptides obtained by hydrolysis of bovine casein by latex extract . J. corumbensis showed activity antimicrobial. This fraction was subjected to reverse phase chromatography for purification of peptides and subsequent mass spectrometric evaluation. The peptides were sequenced and evaluated for their antimicrobial potential. © 2012 Elsevier Ltd.


Rinaldi M.M.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | Sandri D.,State University of Goiás | Oliveira B.N.,State University of Goiás | Sales R.N.,Agencia Rural | Amaral R.D.A.,University of Campinas
Boletim Centro de Pesquisa de Processamento de Alimentos | Year: 2011

The aim of this study was to determine the shelf life post-harvest and kind of packaging of tomato cultivar Dominator, stored in environmental and refrigerated conditions. The product was packed in low-density polyethylene with a thickness of 35 μm and polystyrene trays expanded covered with flexible film of polyvinyl chloride with a thickness of 30 μm. As control treatment was used the product without packaging. The product was kept in environmental conditions at 24 ° C ± 5 ° C and relative humidity of 52% ± 5% and refrigerated conditions at 12°C ± 1 oC and relative humidity of 90% ± 5%. Initially, the tomatoes were analyzed for weight, diameter, length, pulp thickness and size of the vault. At the beginning and at four, seven and nine days of storage, pH, soluble solids, weight loss and sensory analysis was determined, considering the overall appearance, color, texture and flavour. The judges also opined that buy the product or not. The experimental design was completely randomized with three replications. Under the experimental conditions, the longer shelf life of fruit and packaging have been better to polystyrene trays covered with flexible PVC film under refrigeration, for better preserve the product, maintain its physical-chemical and reduce the weight loss cool. Fruits submitted to environmental conditions without package presented considerably shelf life. The major buyng possibility also occurred in fruit subjected to these treatments.


Reis R.C.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | Viana E.S.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | De Jesus J.L.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | Lima L.F.,Federal University of Recôncavo da Bahia | And 2 more authors.
Ciencia Rural | Year: 2015

The objective of this research was to determine the concentration of carotenoids, vitamin C, polyphenols and antioxidant activity of improved varieties of papaya. Four improved varieties from Solo group (L60, L47-05, L47-08 and H54.78), two from Formosa group (L33 and H36.45) and commercial varieties Tainungn.1 and Sunrise Solo were evaluated. Among the Solo group varieties, L47-08 showed high levels of carotenoids and Sunrise Solo the highest content of vitamin C. Among the Formosa group varieties, H36.45 and L33 showed high levels of vitamin C and total polyphenols, as well as higher antioxidant activity. Total polyphenols and vitamin C were significantly correlated with the antioxidant activity of papayas evaluated. © 2015, Universidade Federal de Santa Maria. All rights reserved.


de Souza Viana E.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | Cardoso Reis R.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | de Jesus J.L.,Laboratorio Of Ciencia E Tecnologia Of Alimentos | Theodoro Junghans D.,Embrapa Mandioca e Fruticultura | Duarte Souza F.V.,Laboratorio Of Cultura Of Tecidos
Ciencia Rural | Year: 2013

Five pineapple genotypes (Gold, Pérola, Smooth Cayenne, BRS Vitória and BRS Imperial and four new hybrids -SC x PRI-21, SC48 x PRI-02, PE x SC-73 and PA x PE-01), were evaluated for their physicochemical characteristics. The 'Gold' cultivar presented the highest contents of total carotenoids (7.82mg g-1) and vitamin C (51.0mg 100g-1), whereas the 'BRS' Imperial stood out for its high levels of reducing (5.12%) and total (15.23%) sugars, soluble solids (18.41°Brix), pH (3.96) and ratio (35.28). Improved hybrids SC 48 x PRI-02, PE x SC-73 and PA x PE-01 had the soluble solids, reducing and total sugars contents higher than those of cultivars 'Smooth Cayenne', 'BRS Vitória' and 'Pérola', highlighting the 'PE x SC-73' hybrid for its pulp of intense yellow color, high sweetness, high content of total carotenoids and vitamin C and which was considered as a promising hybrid to replace commercial cultivars.

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