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Dall'Aglio C.,Sezione di Anatomia Veterinaria | Pascucci L.,Sezione di Anatomia Veterinaria | Mercati F.,Sezione di Anatomia Veterinaria | Boiti C.,Laboratorio Of Biotecnologie Fisiologiche | Ceccarelli P.,Sezione di Anatomia Veterinaria
Acta Histochemica | Year: 2012

The aim of the present study was to investigate by immunohistochemistry the presence and distribution of the orexin system in the stomach and gut of fallow deer. Abundant orexin A-positive cells were localized in the middle and basal portions of the mucosal glands of the cardial and fundic regions of the stomach. In the same gastric areas, orexin B-positive cells were also found, mainly localized in the basal portion of glands. In the intestinal tract, orexin-containing cells were occasionally found in the duodenal epithelium and in the rectal intestinal glands. Immunoreactivity for orexin receptors, type 1 and 2 (OX1R and OX2R), was not detected in the same stomach regions. OX1R-immunopositivity was observed in the enteric neuron ganglia localized in the submucosal and muscular intestinal layers, while OX2R-immunopositivity was found close in contact with the cytoplasmic membrane of epithelial cells in the small intestine. © 2011 Elsevier GmbH. Source

Polisca A.,Sezione di Ostetricia e Ginecologia | Boiti C.,Laboratorio Of Biotecnologie Fisiologiche
Acta Histochemica | Year: 2012

The aim of the present study was to investigate the presence and the distribution of leptin (Ob) and its receptor (ObR) in the feline placenta at term by means of immunohistochemical techniques. A few Ob-positive cells were observed scattered in the lamellae of the labyrinthine placenta. These cells had the morphological characteristics typical of the very abundant cells in the placenta of cats that can be considered as being decidual and, in some cases, syncytiotrophoblastic cells. A few ObR-positive cells were observed in the same placental portion and were mainly localized in the lamellae, showing morphological features typical of decidual and syncytiotrophoblastic cells. No other structure of the placenta or the uterine wall showed positive reaction to the antibodies used. Our results confirm what has already been demonstrated in humans and laboratory animals, but not in domestic animals. Together with other emerging data on the secretory activities of the feline placenta, our study underlines its relevance in the production of molecules long known to be involved in appetite control and, probably, with potential effects on the developing fetus. © 2011 Elsevier GmbH. Source

Maranesi M.,Laboratorio Of Biotecnologie Fisiologiche | Boiti C.,Laboratorio Of Biotecnologie Fisiologiche
Peptides | Year: 2012

The aim of the present work was to study, by means of immunohistochemical and RT-PCR techniques, the presence and distribution of immunopositivity for orexin A and B (OXA and OXB) and orexin type 1 and 2 receptors (OX 1R and OX 2R) in the lacrimal gland of sheep as well as the gene expressions for prepro-orexin (PPOX) and cognate receptors. In serial sections, positive staining for OXA and OXB were localized in the same nervous fibers within the connective tissue septa. Positive staining for OX 1R was evidenced in the wall of small arteries while that for OX 2R was observed in the secretory portion of the acinar gland cells with a characteristic localization in the apical cytoplasm. RT-PCR analysis showed the presence of transcripts for PPOX, OX 1R and OX 2R in the sheep lacrimal gland; the gene expression of OX 1R was two-fold greater (p < 0.01) than that of OX 2R. Taken together the present findings raise intriguing questions on the potential role of the orexinergic system in the regulation of lacrimal gland functions that require further investigations. © 2012 Elsevier Inc. All rights reserved. Source

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