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Petrolina de Goiás, Brazil

Kitzberger C.S.G.,Instituto Agronomico Do Parana | Scholz M.B.S.,Instituto Agronomico Do Parana | Pereira L.F.P.,Laboratorio Of Biotecnologia | Benassi M.T.,State University Londrina
Pesquisa Agropecuaria Brasileira | Year: 2013

The objective of this work was to evaluate the influence of genetic diversity on the chemical composition of traditional and modern cultivars of Brazilian arabica coffee. Traditional (Bourbon, Catuaí and Icatu) and modern cultivars (Iapar 59, IPR 98, IPR 99, and IPR 103) were subjected to the same edaphoclimatic conditions, and to standardized post-harvest treatments. Contents of sucrose, reducing sugars, organic acids (quinic, malic, and citric), total phenolic compounds, 5-caffeoylquinic acid, nitrogenous compounds (protein, caffeine, and trigonelline), total lipids, cafestol, and kahweol were determined. Genetic diversity provides variability in coffee composition, allowing the discrimination between traditional and modern cultivars. Modern cultivars have higher contents of malic and 5-caffeoylquinic acids, total lipids, kahweol and trigonelline. The parameters kahweol and the kahweol/cafestol ratio are proposed as discriminators between traditional and modern cultivars, since the introgression of genes from Coffea canephora increase the kahweol content and the values of kahweol/cafestol ratio.

Felix W.J.P.,Federal Rural University of Pernambuco | Felix L.P.,Federal University of Paraiba | Melo N.F.,Laboratorio Of Biotecnologia | Oliveira M.B.M.,Federal University of Pernambuco | And 2 more authors.
Plant Systematics and Evolution | Year: 2011

In this work, the cytotaxonomic implications of the chromosomal characterization of cultivated and native Zephyranthes species described in northeastern Brazil were studied. All individuals had karyotype formed by a set of metacentric chromosomes, in addition to submetacentric and acrocentric chromosomes. In Zephyranthes robusta, 2n = 12 was observed and karyotype with formula 4M + 2SM in somatic cells, representing the most symmetric karyotype among the investigated species. Z. sylvatica showed three different chromosome complement numbers: 2n = 12 with formula 1M + 5SM, 2n = 12 + 1B with 1M + 5SM + (1B), and 2n = 18 formed by cracks. The cultivated species Z. rosea Lindl. presented 2n = 24 with 4M + 7SM + 1A, however Z. grandiflora Lindl. showed the same chromosome number with 2M + 5SM + 5A. Zephyranthes aff. rosea Lindl. presented 2n = 25 with one small metacentric forming a crack in the fourth metacentric pair. Z. brachyandra has 2n = 24 + (1B) and formula 4M + 3SM + 5A + (1B). Z. candida Herb. presented 2n = 38 and karyotype formula 9M + 10SM. In Habranthus itaobinus numerical variation was observed, with the majority of populations showing a chromosome complement composed of 2n = 44 + 1B with 5M + 12SM + 5A + (1B), or 2n = 44 + 3B in a single population. Mechanisms involved in the formation of these karyotypes from chromosomal imbalance data are discussed. Taken together, data from this study only partially confirm previous counts for epithets and further enhance the cytological variability data previously reported for the genus. © 2011 The Author(s).

Felix W.J.P.,Federal Rural University of Pernambuco | Felix L.P.,Federal University of Paraiba | Melo N.F.,Laboratorio Of Biotecnologia | Dutilh J.H.A.,University of Campinas | Carvalho R.,Federal Rural University of Pernambuco
Plant Systematics and Evolution | Year: 2011

Species belonging to the Amaryllidaceae (Zephyranthes and Habranthus) were analyzed by banding with chromomycin A3 (CMA)/4,6-diamidino-2-phenylindole (DAPI) fluorochromes. The patterns of bands were studied in seven species of Zephyranthes Herb. and one of Habranthus Herb. Subterminal and interstitial DAPI+ bands were observed in Z. robusta 2n = 12 and Z. brachyandra 2n = 24. Other species showed no AT-rich heterochromatin. In species with 2n = 12, CMA+ bands were observed on one chromosome pair of Z. robusta and Zephyranthes sp., while in Z. sylvatica an additional small terminal band in the fifth chromosome pair was observed. Z. rosea and Z. grandiflora presented with 2n = 24 and had four CMA+ bands, while in Z. brachyandra, with 2n = 24 + 1B, there were eight interstitial dot bands and a larger terminal band in the short arm of the B chromosome. Z. candida with 2n = 38 presented CMA+ heterochromatin blocks on the long arms of five metacentric pairs and in the short arm of one of the submetacentric pairs; in addition a terminal band was observed on the long arm of one of the homologues of a larger submetacentric pair. H. itaobinus showed a heterozygous pair revealing a strong CMA+ band in only one of the homologues, likely a nucleolus organizing region. Taxonomic implications and karyotype evolution of this group are discussed and correlated with previous data from the literature. © 2011 Springer-Verlag.

Villalon P.,Institute Salud Carlos III | Valdezate S.,Institute Salud Carlos III | Cabezas T.,Laboratorio Of Biotecnologia | Ortega M.,Institute Salud Carlos III | And 4 more authors.
BMC Microbiology | Year: 2015

Background: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period. Results: PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively. Conclusions: PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80. © 2015 Villalón et al.; licensee BioMed Central.

Magarelli G.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | da Silva J.G.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | da Silva J.G.,University of Brasilia | Sousa Filho I.A.D.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | And 4 more authors.
Microchemical Journal | Year: 2013

A highly sensitive and selective differential pulse voltammetric (DPV) method based on the oxidation of caffeic acid in a glassy carbon electrode is presented for determination of total phenolic acids (TPAs). Under optimized conditions (0.2M phosphate buffer, pH 3, 50mV pulse amplitude, 50mVs-1 scan rate), the oxidation peak current (Ipa) of caffeic acid is linear (Ipa(A)=-4.15×10-8+0.97 [Caffeic acid]) to caffeic acid concentration in the range from 1.0×10-7 to 1.0×10-6M, with a correlation coefficient of 0.9985. The detection and quantitation limits obtained were 6.8×10-8M and 1.0×10-7M, respectively. The repeatability and intermediate precision of DPV method were acceptable (Relative Standard Deviation (RSD) <10%). The absence of the matrix effect on the determination of TPAs by DPV method was attested by F-test and t-test (95% confidence level). The method was successfully applied to the determination of TPAs in five cotton cultivars, with recoveries of 94-104% (RSD <2%). © 2012 Elsevier B.V.

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