Laboratorio Of Biotecnologia

Campina Grande, Brazil

Laboratorio Of Biotecnologia

Campina Grande, Brazil
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Pereira W.J.,Federal University of Goais | Pereira W.J.,University of Brasilia | Bassinello P.Z.,Laboratorio Of Graos E Subprodutos | Brondani C.,Laboratorio Of Biotecnologia | Vianello R.P.,Laboratorio Of Biotecnologia
Crop Breeding and Applied Biotechnology | Year: 2017

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris. © 2017, Brazilian Society of Plant Breeding. All rights reserved.


Maggioli G.,Institute Higiene | Acosta D.,Institute Higiene | Silveira F.,Institute Higiene | Rossi S.,Laboratorio Of Biotecnologia | And 5 more authors.
Vaccine | Year: 2011

Fasciola hepatica M17 leucine aminopeptidase (FhLAP) is thought to play a role in catabolizing peptides generated by the concerted activity of gut-associated endopeptidases on host polypeptides, thus releasing amino acids to be used in protein anabolism. In this study, a recombinant functional form of this homo hexameric metallopeptidase produced in Escherichia coli was used in combination with adjuvants of different types in a vaccination trial in Corriedale sheep against experimental challenge with F. hepatica metacercariae. The experimental assay consisted of 6 groups of 10 animals; 5 of the groups (1-5) were subcutaneously inoculated at weeks 0 and 4 with100 μg of r. FhLAP mixed with Freund's complete plus incomplete adjuvant (group 1), Alum (group 2), Adyuvac 50 (group 3), DEAE-D (group 4) and Ribi (group 5); the adjuvant control group (group 6) received Freund's adjuvant. Two weeks after the booster, the sheep were orally challenged with 200 metacercariae. Immunization with r. FhLAP induced significant reduction in fluke burdens in all vaccinated groups: 83.8% in the Freund's group, 86.7% in the Alum group, 74.4% in the Adyuvac 50 group, 49.8% in the Ribi group and 49.5% in the DEAE-D group compared to the adjuvant control group. Morphometric analysis of recovered liver flukes showed no significant size modifications in the different vaccination groups. All vaccine preparations elicited specific IgG, IgG1 and IgG2 responses. This study shows that a liver fluke vaccine based on r. FhLAP combined with different adjuvants significantly reduced worm burden in a ruminant species that was high in animals that received the enzyme along with the commercially approved adjuvants Alum and Adyuvac 50. © 2011 Elsevier Ltd.


Felix W.J.P.,Federal Rural University of Pernambuco | Felix L.P.,Federal University of Paraiba | Melo N.F.,Laboratorio Of Biotecnologia | Oliveira M.B.M.,Federal University of Pernambuco | And 2 more authors.
Plant Systematics and Evolution | Year: 2011

In this work, the cytotaxonomic implications of the chromosomal characterization of cultivated and native Zephyranthes species described in northeastern Brazil were studied. All individuals had karyotype formed by a set of metacentric chromosomes, in addition to submetacentric and acrocentric chromosomes. In Zephyranthes robusta, 2n = 12 was observed and karyotype with formula 4M + 2SM in somatic cells, representing the most symmetric karyotype among the investigated species. Z. sylvatica showed three different chromosome complement numbers: 2n = 12 with formula 1M + 5SM, 2n = 12 + 1B with 1M + 5SM + (1B), and 2n = 18 formed by cracks. The cultivated species Z. rosea Lindl. presented 2n = 24 with 4M + 7SM + 1A, however Z. grandiflora Lindl. showed the same chromosome number with 2M + 5SM + 5A. Zephyranthes aff. rosea Lindl. presented 2n = 25 with one small metacentric forming a crack in the fourth metacentric pair. Z. brachyandra has 2n = 24 + (1B) and formula 4M + 3SM + 5A + (1B). Z. candida Herb. presented 2n = 38 and karyotype formula 9M + 10SM. In Habranthus itaobinus numerical variation was observed, with the majority of populations showing a chromosome complement composed of 2n = 44 + 1B with 5M + 12SM + 5A + (1B), or 2n = 44 + 3B in a single population. Mechanisms involved in the formation of these karyotypes from chromosomal imbalance data are discussed. Taken together, data from this study only partially confirm previous counts for epithets and further enhance the cytological variability data previously reported for the genus. © 2011 The Author(s).


PubMed | Laboratorio Of Biotecnologia, National Autonomous University of Mexico and Servicio Of Epidemiologia Del Hospital Central Dr Ignacio Morones Prieto
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2015

Infections arising from bacterial adhesion and colonization on chronic wounds are a significant healthcare problem. Silver nanoparticles (AgNPs) impregnated in dressing have attracted a great deal of attention as a potential solution. The goal of the present study was to evaluate the anti-biofilm activities of AgNPs impregnated in commercial dressings against Pseudomonas aeruginosa, bacteria isolated of chronic wounds from a hospital patient. The antimicrobial activity of AgNPs was tested within biofilms generated under slow fluid shear conditions using a standard bioreactor. A 2-log reduction in the number of colony-forming units of P. aeruginosa was recorded in the reactor on exposure to dressing impregnated with 250ppm of AgNPs, diameter 9.31.1nm, and also showed compatibility to mammalian cells (human fibroblasts). Our study suggests that the use of dressings with AgNPs may either prevent or reduce microbial growth in the wound environment, and reducing wound bioburden may improve wound-healing outcomes.


Haller H.,Mid Sweden University | Jonsson A.,Mid Sweden University | Lacayo Romero M.,Laboratorio Of Biotecnologia | Jarquin Pascua M.,Laboratorio Of Biotecnologia
Ecological Engineering | Year: 2017

Soils polluted by persistent organic pollutants threatens habitats for plants and animals as well as basic human needs such as food security and provision of potable water. Toxaphene is a persistent organic pollutant that was heavily used as a pesticide in Central America, Brazil, Soviet Union etc. until it was banned in 1993. The objective of this study was to determine the bioaccumulation and translocation characteristics of three different cultivars of amaranth in soils contaminated with field-weathered toxaphene and other POPs in former cotton fields in Chinandega, Nicaragua to identify safety issues for human consumption and/or potential for phytoremediation. The concentration of toxaphene and other POPs in the edible parts of the amaranth (leaves and seeds) exceeded the maximum residue level for human consumption established by the European Union for most of the tested compounds. Concentrations of toxaphene congeners and other POPs were found in all vegetative organs. Many substances were accumulated to concentrations more than 10 times higher than in the soil. Of the three cultivars, A. caudatus 'CAC48 Perú' and A. cruentus 'Don Leon' presented the highest average BAF. None of the 3 cultivars can be considered a panacea hyperaccumulator for either toxaphene alone or in conjunction with other POPs but since many agricultural soils comprise a range of different contaminants, the broad-spectrum bioaccumulating capacity of amaranth can make it an interesting candidate for phytoremediation. © 2017 Elsevier B.V.


Kitzberger C.S.G.,Instituto Agronomico do Parana | Scholz M.B.S.,Instituto Agronomico do Parana | Pereira L.F.P.,Laboratorio Of Biotecnologia | Benassi M.T.,State University Londrina
Pesquisa Agropecuaria Brasileira | Year: 2013

The objective of this work was to evaluate the influence of genetic diversity on the chemical composition of traditional and modern cultivars of Brazilian arabica coffee. Traditional (Bourbon, Catuaí and Icatu) and modern cultivars (Iapar 59, IPR 98, IPR 99, and IPR 103) were subjected to the same edaphoclimatic conditions, and to standardized post-harvest treatments. Contents of sucrose, reducing sugars, organic acids (quinic, malic, and citric), total phenolic compounds, 5-caffeoylquinic acid, nitrogenous compounds (protein, caffeine, and trigonelline), total lipids, cafestol, and kahweol were determined. Genetic diversity provides variability in coffee composition, allowing the discrimination between traditional and modern cultivars. Modern cultivars have higher contents of malic and 5-caffeoylquinic acids, total lipids, kahweol and trigonelline. The parameters kahweol and the kahweol/cafestol ratio are proposed as discriminators between traditional and modern cultivars, since the introgression of genes from Coffea canephora increase the kahweol content and the values of kahweol/cafestol ratio.


de Sousa Trindade M.J.,Laboratorio Of Biotecnologia | Alves Lameira O.,Laboratorio Of Biotecnologia
Revista Cubana de Plantas Medicinales | Year: 2014

Introduction: Euphorbiaceae is one of the largest, most complex and most diverse botanical families. With more than 1 100 species, either native or naturalized in Brazil, this family has a great potential for use. Euphorbiaceae species have great economic importance as foods or drugs of popular use. Objective: broaden knowledge about the assigned Euphorbiaceae species, thus contributing to improve their use as drugs. Methods: a bibliographic review was conducted of books, websites and scientific papers dealing with the use of the assigned species of the Euphorbiaceae family and published from 1824 to 2012. Results: data were obtained about 149 species and 35 genera with a variety of uses, most of which were medicinal. The genus with the largest number of species used was Croton with 58, followed by Euphorbia with 13 and Jatropha with 9. Conclusion: the studies show great viability in the use of species from the Euphorbiaceae family. © 2015, Editorial Ciencias Medicas. All rights reserved.


Haller H.,Mid Sweden University | Jonsson A.,Mid Sweden University | Rayo K.M.,Laboratorio Of Biotecnologia | Lopez A.D.,Laboratorio Of Biotecnologia
International Biodeterioration and Biodegradation | Year: 2016

Soil bioaugmentation is a promising approach with applications in agriculture and in bioremediation of polluted soil. One way of supplying microorganisms to the soil that has received increasing attention during the last decade is by the addition of compost teas. The success of such strategies depends on whether the organisms are capable of migrating through the soil and reaching its target, i.e. the pollutants or soil pathogens. The structural conditions of the soil affect the microbial migration rate and this study aims to determine the migration rates of microorganisms found in aerated compost tea through both a sandy loam and a clay loam in soil columns. A considerably higher proportion of the microorganisms from the aerated compost tea were deposited at 2 cm in the sandy loam compared to the clay loam. Microbial deposition was concentrated to the top 2 cm in the sandy loam and then decreased abruptly at 10 cm whereas the clay loam presented an irregular pattern. Despite a favourable particle size distribution for microbial transport, the sandy loam retained a greater proportion of the microorganisms present in the aerated compost tea in the top 2 cm, presumably because the lower bulk density and higher soil organic matter in the clay loam aided transport and growth of microorganisms. The limited migration of aerated compost tea microorganisms in sandy soil suggests that its efficiency for bioremediation or pathogen control may be limited below 2 cm depth in similar soils. Our results indicate that in less dense soil with higher soil organic matter content the efficiency may be higher. © 2015 Elsevier Ltd.


PubMed | Laboratorio Of Biotecnologia, Federal University of Goais, Instituto Federal Goiano IF Goiano and University of Brasilia
Type: Journal Article | Journal: Molecular genetics and genomics : MGG | Year: 2016

Researchers have made great advances into the development and application of genomic approaches for common beans, creating opportunities to driving more real and applicable strategies for sustainable management of the genetic resource towards plant breeding. This work provides useful polymorphic single-nucleotide polymorphisms (SNPs) for high-throughput common bean genotyping developed by RAD (restriction site-associated DNA) sequencing. The RAD tags were generated from DNA pooled from 12 common bean genotypes, including breeding lines of different gene pools and market classes. The aligned sequences identified 23,748 putative RAD-SNPs, of which 3357 were adequate for genotyping; 1032 RAD-SNPs with the highest ADT (assay design tool) score are presented in this article. The RAD-SNPs were structurally annotated in different coding (47.00%) and non-coding (53.00%) sequence components of genes. A subset of 384 RAD-SNPs with broad genome distribution was used to genotype a diverse panel of 95 common bean germplasms and revealed a successful amplification rate of 96.6%, showing 73% of polymorphic SNPs within the Andean group and 83% in the Mesoamerican group. A slightly increased He (0.161, n=21) value was estimated for the Andean gene pool, compared to the Mesoamerican group (0.156, n=74). For the linkage disequilibrium (LD) analysis, from a group of 580 SNPs (289 RAD-SNPs and 291 BARC-SNPs) genotyped for the same set of genotypes, 70.2% were in LD, decreasing to 0.10%in the Andean group and 0.77% in the Mesoamerican group. Haplotype patterns spanning 310Mb of the genome (60%) were characterized in samples from different origins. However, the haplotype frameworks were under-represented for the Andean (7.85%) and Mesoamerican (5.55%) gene pools separately. In conclusion, RAD sequencing allowed the discovery of hundreds of useful SNPs for broad genetic analysis of common bean germplasm. From now, this approach provides an excellent panel of molecular tools for whole genome analysis, allowing integrating and better exploring the common bean breeding practices.


PubMed | Laboratorio Of Biotecnologia and Instituto Federal Goiano
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridization with exotic species of cotton. In this study, we investigated the rate of natural hybridization in two populations of G. mustelinum in Bahia, Brazil, with G. hirsutum and G. barbadense using a set of microsatellite markers.

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