Laboratorio Of Biotecnologia

Campina Grande, Brazil

Laboratorio Of Biotecnologia

Campina Grande, Brazil
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Maggioli G.,Institute Higiene | Acosta D.,Institute Higiene | Silveira F.,Institute Higiene | Rossi S.,Laboratorio Of Biotecnologia | And 5 more authors.
Vaccine | Year: 2011

Fasciola hepatica M17 leucine aminopeptidase (FhLAP) is thought to play a role in catabolizing peptides generated by the concerted activity of gut-associated endopeptidases on host polypeptides, thus releasing amino acids to be used in protein anabolism. In this study, a recombinant functional form of this homo hexameric metallopeptidase produced in Escherichia coli was used in combination with adjuvants of different types in a vaccination trial in Corriedale sheep against experimental challenge with F. hepatica metacercariae. The experimental assay consisted of 6 groups of 10 animals; 5 of the groups (1-5) were subcutaneously inoculated at weeks 0 and 4 with100 μg of r. FhLAP mixed with Freund's complete plus incomplete adjuvant (group 1), Alum (group 2), Adyuvac 50 (group 3), DEAE-D (group 4) and Ribi (group 5); the adjuvant control group (group 6) received Freund's adjuvant. Two weeks after the booster, the sheep were orally challenged with 200 metacercariae. Immunization with r. FhLAP induced significant reduction in fluke burdens in all vaccinated groups: 83.8% in the Freund's group, 86.7% in the Alum group, 74.4% in the Adyuvac 50 group, 49.8% in the Ribi group and 49.5% in the DEAE-D group compared to the adjuvant control group. Morphometric analysis of recovered liver flukes showed no significant size modifications in the different vaccination groups. All vaccine preparations elicited specific IgG, IgG1 and IgG2 responses. This study shows that a liver fluke vaccine based on r. FhLAP combined with different adjuvants significantly reduced worm burden in a ruminant species that was high in animals that received the enzyme along with the commercially approved adjuvants Alum and Adyuvac 50. © 2011 Elsevier Ltd.

Felix W.J.P.,Federal Rural University of Pernambuco | Felix L.P.,Federal University of Paraiba | Melo N.F.,Laboratorio Of Biotecnologia | Oliveira M.B.M.,Federal University of Pernambuco | And 2 more authors.
Plant Systematics and Evolution | Year: 2011

In this work, the cytotaxonomic implications of the chromosomal characterization of cultivated and native Zephyranthes species described in northeastern Brazil were studied. All individuals had karyotype formed by a set of metacentric chromosomes, in addition to submetacentric and acrocentric chromosomes. In Zephyranthes robusta, 2n = 12 was observed and karyotype with formula 4M + 2SM in somatic cells, representing the most symmetric karyotype among the investigated species. Z. sylvatica showed three different chromosome complement numbers: 2n = 12 with formula 1M + 5SM, 2n = 12 + 1B with 1M + 5SM + (1B), and 2n = 18 formed by cracks. The cultivated species Z. rosea Lindl. presented 2n = 24 with 4M + 7SM + 1A, however Z. grandiflora Lindl. showed the same chromosome number with 2M + 5SM + 5A. Zephyranthes aff. rosea Lindl. presented 2n = 25 with one small metacentric forming a crack in the fourth metacentric pair. Z. brachyandra has 2n = 24 + (1B) and formula 4M + 3SM + 5A + (1B). Z. candida Herb. presented 2n = 38 and karyotype formula 9M + 10SM. In Habranthus itaobinus numerical variation was observed, with the majority of populations showing a chromosome complement composed of 2n = 44 + 1B with 5M + 12SM + 5A + (1B), or 2n = 44 + 3B in a single population. Mechanisms involved in the formation of these karyotypes from chromosomal imbalance data are discussed. Taken together, data from this study only partially confirm previous counts for epithets and further enhance the cytological variability data previously reported for the genus. © 2011 The Author(s).

PubMed | Laboratorio Of Biotecnologia, National Autonomous University of Mexico and Servicio Of Epidemiologia Del Hospital Central Dr Ignacio Morones Prieto
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2015

Infections arising from bacterial adhesion and colonization on chronic wounds are a significant healthcare problem. Silver nanoparticles (AgNPs) impregnated in dressing have attracted a great deal of attention as a potential solution. The goal of the present study was to evaluate the anti-biofilm activities of AgNPs impregnated in commercial dressings against Pseudomonas aeruginosa, bacteria isolated of chronic wounds from a hospital patient. The antimicrobial activity of AgNPs was tested within biofilms generated under slow fluid shear conditions using a standard bioreactor. A 2-log reduction in the number of colony-forming units of P. aeruginosa was recorded in the reactor on exposure to dressing impregnated with 250ppm of AgNPs, diameter 9.31.1nm, and also showed compatibility to mammalian cells (human fibroblasts). Our study suggests that the use of dressings with AgNPs may either prevent or reduce microbial growth in the wound environment, and reducing wound bioburden may improve wound-healing outcomes.

Kitzberger C.S.G.,Instituto Agronomico do Parana | Scholz M.B.S.,Instituto Agronomico do Parana | Pereira L.F.P.,Laboratorio Of Biotecnologia | Benassi M.T.,State University Londrina
Pesquisa Agropecuaria Brasileira | Year: 2013

The objective of this work was to evaluate the influence of genetic diversity on the chemical composition of traditional and modern cultivars of Brazilian arabica coffee. Traditional (Bourbon, Catuaí and Icatu) and modern cultivars (Iapar 59, IPR 98, IPR 99, and IPR 103) were subjected to the same edaphoclimatic conditions, and to standardized post-harvest treatments. Contents of sucrose, reducing sugars, organic acids (quinic, malic, and citric), total phenolic compounds, 5-caffeoylquinic acid, nitrogenous compounds (protein, caffeine, and trigonelline), total lipids, cafestol, and kahweol were determined. Genetic diversity provides variability in coffee composition, allowing the discrimination between traditional and modern cultivars. Modern cultivars have higher contents of malic and 5-caffeoylquinic acids, total lipids, kahweol and trigonelline. The parameters kahweol and the kahweol/cafestol ratio are proposed as discriminators between traditional and modern cultivars, since the introgression of genes from Coffea canephora increase the kahweol content and the values of kahweol/cafestol ratio.

de Sousa Trindade M.J.,Laboratorio Of Biotecnologia | Alves Lameira O.,Laboratorio Of Biotecnologia
Revista Cubana de Plantas Medicinales | Year: 2014

Introduction: Euphorbiaceae is one of the largest, most complex and most diverse botanical families. With more than 1 100 species, either native or naturalized in Brazil, this family has a great potential for use. Euphorbiaceae species have great economic importance as foods or drugs of popular use. Objective: broaden knowledge about the assigned Euphorbiaceae species, thus contributing to improve their use as drugs. Methods: a bibliographic review was conducted of books, websites and scientific papers dealing with the use of the assigned species of the Euphorbiaceae family and published from 1824 to 2012. Results: data were obtained about 149 species and 35 genera with a variety of uses, most of which were medicinal. The genus with the largest number of species used was Croton with 58, followed by Euphorbia with 13 and Jatropha with 9. Conclusion: the studies show great viability in the use of species from the Euphorbiaceae family. © 2015, Editorial Ciencias Medicas. All rights reserved.

Haller H.,Mid Sweden University | Jonsson A.,Mid Sweden University | Rayo K.M.,Laboratorio Of Biotecnologia | Lopez A.D.,Laboratorio Of Biotecnologia
International Biodeterioration and Biodegradation | Year: 2016

Soil bioaugmentation is a promising approach with applications in agriculture and in bioremediation of polluted soil. One way of supplying microorganisms to the soil that has received increasing attention during the last decade is by the addition of compost teas. The success of such strategies depends on whether the organisms are capable of migrating through the soil and reaching its target, i.e. the pollutants or soil pathogens. The structural conditions of the soil affect the microbial migration rate and this study aims to determine the migration rates of microorganisms found in aerated compost tea through both a sandy loam and a clay loam in soil columns. A considerably higher proportion of the microorganisms from the aerated compost tea were deposited at 2 cm in the sandy loam compared to the clay loam. Microbial deposition was concentrated to the top 2 cm in the sandy loam and then decreased abruptly at 10 cm whereas the clay loam presented an irregular pattern. Despite a favourable particle size distribution for microbial transport, the sandy loam retained a greater proportion of the microorganisms present in the aerated compost tea in the top 2 cm, presumably because the lower bulk density and higher soil organic matter in the clay loam aided transport and growth of microorganisms. The limited migration of aerated compost tea microorganisms in sandy soil suggests that its efficiency for bioremediation or pathogen control may be limited below 2 cm depth in similar soils. Our results indicate that in less dense soil with higher soil organic matter content the efficiency may be higher. © 2015 Elsevier Ltd.

PubMed | Laboratorio Of Biotecnologia, Federal University of Goais, Instituto Federal Goiano IF Goiano and University of Brasilia
Type: Journal Article | Journal: Molecular genetics and genomics : MGG | Year: 2016

Researchers have made great advances into the development and application of genomic approaches for common beans, creating opportunities to driving more real and applicable strategies for sustainable management of the genetic resource towards plant breeding. This work provides useful polymorphic single-nucleotide polymorphisms (SNPs) for high-throughput common bean genotyping developed by RAD (restriction site-associated DNA) sequencing. The RAD tags were generated from DNA pooled from 12 common bean genotypes, including breeding lines of different gene pools and market classes. The aligned sequences identified 23,748 putative RAD-SNPs, of which 3357 were adequate for genotyping; 1032 RAD-SNPs with the highest ADT (assay design tool) score are presented in this article. The RAD-SNPs were structurally annotated in different coding (47.00%) and non-coding (53.00%) sequence components of genes. A subset of 384 RAD-SNPs with broad genome distribution was used to genotype a diverse panel of 95 common bean germplasms and revealed a successful amplification rate of 96.6%, showing 73% of polymorphic SNPs within the Andean group and 83% in the Mesoamerican group. A slightly increased He (0.161, n=21) value was estimated for the Andean gene pool, compared to the Mesoamerican group (0.156, n=74). For the linkage disequilibrium (LD) analysis, from a group of 580 SNPs (289 RAD-SNPs and 291 BARC-SNPs) genotyped for the same set of genotypes, 70.2% were in LD, decreasing to 0.10%in the Andean group and 0.77% in the Mesoamerican group. Haplotype patterns spanning 310Mb of the genome (60%) were characterized in samples from different origins. However, the haplotype frameworks were under-represented for the Andean (7.85%) and Mesoamerican (5.55%) gene pools separately. In conclusion, RAD sequencing allowed the discovery of hundreds of useful SNPs for broad genetic analysis of common bean germplasm. From now, this approach provides an excellent panel of molecular tools for whole genome analysis, allowing integrating and better exploring the common bean breeding practices.

PubMed | Laboratorio Of Biotecnologia and Instituto Federal Goiano
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridization with exotic species of cotton. In this study, we investigated the rate of natural hybridization in two populations of G. mustelinum in Bahia, Brazil, with G. hirsutum and G. barbadense using a set of microsatellite markers.

PubMed | Federal University of Goais, Laboratorio Of Biotecnologia, Nucleo de Biotecnologia Aplicada and North Dakota State University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Gene expression related to drought response in the leaf tissues of two Brazilian upland cultivars, the drought-tolerant Dourado and the drought-sensitive Primavera, was analyzed. RNA-seq identified 27,618 transcripts in the Dourado cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the Primavera cultivar, with 23,663 (86.9%) homologous to the rice database. Gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differentially expressed genes in Dourado and Primavera cultivars, respectively. Genes exclusively expressed under drought were identified for Dourado, including two genes of particular interest coding for the protein peroxidase precursor, which is involved in three distinct metabolic pathways. Comparisons between the two drought-exposed cultivars revealed 2314 genes were differentially expressed (978 upregulated, 1336 downregulated in Dourado). Six genes distributed across 4 different transcription factor families (bHLH, MYB, NAC, and WRKY) were identified, all of which were upregulated in Dourado compared to Primavera during drought. Most of the genes identified in Dourado activate metabolic pathways responsible for production of secondary metabolites and genes coding for enzymatically active signaling receptors. Quantitative PCR validation showed that most gene expression was in agreement with computational prediction of these transcripts. The transcripts identified here will define molecular markers for identification of Cis-acting elements to search for allelic variants of these genes through analysis of polymorphic SNPs in GenBank accessions of upland rice, aiming to develop cultivars with the best combination of these alleles, resulting in materials with high yield potential in the event of drought during the reproductive phase.

Magarelli G.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | da Silva J.G.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | da Silva J.G.,University of Brasilia | Sousa Filho I.A.D.,Laboratorio Of Tecnologias Para A Seguranca Alimentar | And 4 more authors.
Microchemical Journal | Year: 2013

A highly sensitive and selective differential pulse voltammetric (DPV) method based on the oxidation of caffeic acid in a glassy carbon electrode is presented for determination of total phenolic acids (TPAs). Under optimized conditions (0.2M phosphate buffer, pH 3, 50mV pulse amplitude, 50mVs-1 scan rate), the oxidation peak current (Ipa) of caffeic acid is linear (Ipa(A)=-4.15×10-8+0.97 [Caffeic acid]) to caffeic acid concentration in the range from 1.0×10-7 to 1.0×10-6M, with a correlation coefficient of 0.9985. The detection and quantitation limits obtained were 6.8×10-8M and 1.0×10-7M, respectively. The repeatability and intermediate precision of DPV method were acceptable (Relative Standard Deviation (RSD) <10%). The absence of the matrix effect on the determination of TPAs by DPV method was attested by F-test and t-test (95% confidence level). The method was successfully applied to the determination of TPAs in five cotton cultivars, with recoveries of 94-104% (RSD <2%). © 2012 Elsevier B.V.

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