Laboratorio Of Biologia Of Insetos E Controle Biologico

Pelotas, Brazil

Laboratorio Of Biologia Of Insetos E Controle Biologico

Pelotas, Brazil

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Barbosa-Negrisoli C.R.C.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Negrisoli A.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Dolinski C.,State University of Norte Fluminense | Bernardi D.,Laboratorio Of Biologia Of Insetos E Controle Biologico
Crop Protection | Year: 2010

The Brazilian apple leafroller, Bonagota salubricola (Meyrick, 1937) (Bonagota cranaodes) (Lepidoptera: Tortricidae), is one of the main pest problems in apple orchards is Brazil. The purpose of this work was to evaluate B. salubricola larval and pupal susceptibility to different species of entomopathogenic nematodes, isolated in Rio Grande do Sul state in Brazil, under laboratory and field (apple orchard) conditions. Bioassays for isolates selection and determination of lethal concentration were performed in tubes of 1.5ml (eppendorf), each containing one B. salubricola third instar larvae and filter paper. Field experiments were performed in commercial orchard, with application of 100 infecting juveniles (IJs)/cm2 for each apple plant previously infected with five B. salubricola larvae covered with plastic trays containing thin cloth. Nematodes H. bacteriophora RS107 and H. bacteriophora RS57 had LC50 of 13 and 4.5IJs/larvae (4.3 and 1.5IJs/cm2), respectively. In the field, H. bacteriophora RS107 and H. bacteriophora RS57, applied with sorbitol as an adjuvant, reached 70.2 and 61.1% larval mortality, respectively. The results showed that both isolates had biological activity against B. salubricola under laboratory and field conditions. © 2010 Elsevier Ltd.


Negrisoli A.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Garcia M.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Barbosa Negrisoli C.R.C.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Bernardi D.,Laboratorio Of Biologia Of Insetos E Controle Biologico | da Silva A.,Laboratorio Of Biologia Of Insetos E Controle Biologico
Crop Protection | Year: 2010

The main insect pest in Brazilian corn is fall armyworm, Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae). Entomopathogenic nematodes (EPNs) can be used to control this pest, and can be applied together with various insecticides. Thus, the objective of this work was to evaluate the efficacy of mixtures of EPNs and insecticides to control S. frugiperda in corn crops. In laboratory bioassays three species of EPNs were tested (Heterorhabditis indica, Steinernema carpocapsae and Steinernema glaseri) together with 18 registered insecticides to control S. frugiperda in corn. Efficacy of association between insecticides and EPNs on S. frugiperda larvae was evaluated against the insect's third instar, 2 and 4 days after applications in laboratory. Experiments in the field were performed in two consecutive years, with located application of H. indica and S. carpocapsae (250 IJs/cm2) mixed with chlorpyrifos (0.3 L/ha) and lufenuron (0.15 L/ha) on the corn husk. In laboratory, after two days exposure the interaction between chlorpyrifos and H. indica was synergistic, while interaction with cypermethrin, spinosad, methoxyfenozide and deltamethrin + triazofos was additive, as was interaction between lufenuron, chlorpyrifos and cypermethrin with S. carpocapsae. In contrast, the interaction between chlorpyrifos (Vexter™ and Lorsban™ and lufenuron with S. glaseri was synergistic. In the field, the best treatment was the mixture of H. indica with lufenuron (0.15 L/ha), with 62.5% and 57.5% larval mortality in the two evaluation years in the field, respectively. © 2010 Elsevier Ltd.

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