Laboratorio Of Biologia Of Insetos E Controle Biologico

Pelotas, Brazil

Laboratorio Of Biologia Of Insetos E Controle Biologico

Pelotas, Brazil
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De Carvalho Barbosa Negrisoli C.R.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Negrisoli A.S.,Embrapa CPATC UEP Rio Largo | Garcia M.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Dolinski C.,State University of Norte Fluminense | Bernardi D.,Laboratorio Of Biologia Of Insetos E Controle Biologico
Experimental Parasitology | Year: 2013

Oriental fruit moth Grapholita molesta (Busck, 1916) (Lepidoptera: Tortricidae) is considered a major pest in temperate fruit trees, such as peach and apple. Entomopathogenic nematodes (EPNs) are regarded as viable for pest management control due to their efficiency against tortricid in these trees. The objective of this study was to evaluate the effectiveness of native EPNs from Rio Grande do Sul state against pre-pupae of G. molesta under laboratory and field conditions. In the laboratory, pre-pupae of G. molesta were placed in corrugated cardboard sheets inside glass tubes and exposed to 17 different EPNs strains at concentrations of 6, 12, 24, 48 and 60IJs/cm2 and maintained at 25°C, 70±10% RH and photophase of 16h. Insect mortality was recorded 72h after inoculation of EPNs. Steinernema rarum RS69 and Heterorhabditis bacteriophora RS33 were the most virulent strains and selected for field application (LC95 of 70.5 and 53.8IJs/cm2, respectively). Both strains were highly efficient under field conditions when applied in aqueous suspension directed to larvae on peach tree trunk, causing mortality of 94 and 97.0%, respectively. © 2013.


Barbosa-Negrisoli C.R.C.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Negrisoli A.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Dolinski C.,State University of Norte Fluminense | Bernardi D.,Laboratorio Of Biologia Of Insetos E Controle Biologico
Crop Protection | Year: 2010

The Brazilian apple leafroller, Bonagota salubricola (Meyrick, 1937) (Bonagota cranaodes) (Lepidoptera: Tortricidae), is one of the main pest problems in apple orchards is Brazil. The purpose of this work was to evaluate B. salubricola larval and pupal susceptibility to different species of entomopathogenic nematodes, isolated in Rio Grande do Sul state in Brazil, under laboratory and field (apple orchard) conditions. Bioassays for isolates selection and determination of lethal concentration were performed in tubes of 1.5ml (eppendorf), each containing one B. salubricola third instar larvae and filter paper. Field experiments were performed in commercial orchard, with application of 100 infecting juveniles (IJs)/cm2 for each apple plant previously infected with five B. salubricola larvae covered with plastic trays containing thin cloth. Nematodes H. bacteriophora RS107 and H. bacteriophora RS57 had LC50 of 13 and 4.5IJs/larvae (4.3 and 1.5IJs/cm2), respectively. In the field, H. bacteriophora RS107 and H. bacteriophora RS57, applied with sorbitol as an adjuvant, reached 70.2 and 61.1% larval mortality, respectively. The results showed that both isolates had biological activity against B. salubricola under laboratory and field conditions. © 2010 Elsevier Ltd.


Negrisoli A.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Garcia M.S.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Barbosa Negrisoli C.R.C.,Laboratorio Of Biologia Of Insetos E Controle Biologico | Bernardi D.,Laboratorio Of Biologia Of Insetos E Controle Biologico | da Silva A.,Laboratorio Of Biologia Of Insetos E Controle Biologico
Crop Protection | Year: 2010

The main insect pest in Brazilian corn is fall armyworm, Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae). Entomopathogenic nematodes (EPNs) can be used to control this pest, and can be applied together with various insecticides. Thus, the objective of this work was to evaluate the efficacy of mixtures of EPNs and insecticides to control S. frugiperda in corn crops. In laboratory bioassays three species of EPNs were tested (Heterorhabditis indica, Steinernema carpocapsae and Steinernema glaseri) together with 18 registered insecticides to control S. frugiperda in corn. Efficacy of association between insecticides and EPNs on S. frugiperda larvae was evaluated against the insect's third instar, 2 and 4 days after applications in laboratory. Experiments in the field were performed in two consecutive years, with located application of H. indica and S. carpocapsae (250 IJs/cm2) mixed with chlorpyrifos (0.3 L/ha) and lufenuron (0.15 L/ha) on the corn husk. In laboratory, after two days exposure the interaction between chlorpyrifos and H. indica was synergistic, while interaction with cypermethrin, spinosad, methoxyfenozide and deltamethrin + triazofos was additive, as was interaction between lufenuron, chlorpyrifos and cypermethrin with S. carpocapsae. In contrast, the interaction between chlorpyrifos (Vexter™ and Lorsban™ and lufenuron with S. glaseri was synergistic. In the field, the best treatment was the mixture of H. indica with lufenuron (0.15 L/ha), with 62.5% and 57.5% larval mortality in the two evaluation years in the field, respectively. © 2010 Elsevier Ltd.

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