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Campos dos Goytacazes, Brazil

Baldotto L.E.B.,State University of Norte Fluminense | Baldotto M.A.,Laboratorio Of Solos | Olivares F.L.,Laboratorio Of Biologia Celular E Tecidual | Viana A.P.,Laboratorio Of Genetica E Melhoramento Of Plantas | Bressan-Smith R.,Laboratorio Of Genetica E Melhoramento Of Plantas
Revista Brasileira de Ciencia do Solo | Year: 2010

Pineapple (Ananas comosus L. Merrill) in vitro propagation results in the production of a large quantity of healthy and homogeneous plantlets. Despite these advantages, the long time required for acclimatization makes this agricultural practice too costly. Growth acceleration of plantlets by inoculation with endophytic and epiphytic diazotrophic bacteria may be useful to reduce this period. The objectives of this study were to evaluate the potential of 20 diazotrophic bacteria strains in synthesizing indole, solubilizing Ca phosphate and Zn oxide and acting against the fungus Fusarium subglutinans f. sp. ananas, and subsequently, evaluate the performance of pineapple 'Vitória' propagated by tissue culture in response to the application of these isolates during the acclimatization period in a greenhouse. Shoot and root growth characteristics and leaf nutrient content of pineapple were evaluated. Results showed differences in the ability of the bacteria strains to synthesize indole, solubilize Ca phosphate and Zn oxide and act against Fusarium. Differences in the growth-promoting capacity of the shoot and root system of bacteria and leaf accumulation of N, P, K, Ca and Mg in pineapple were also shown. Inoculation with diazotrophic bacteria can promote the growth of plantlets during the acclimatization period, improving the adaptation of pineapple to the exvitro environment. Source

Vale E.M.,State University of Norte Fluminense | Reis R.S.,State University of Norte Fluminense | Santa-Catarina C.,Laboratorio Of Biologia Celular E Tecidual | Silveira V.,State University of Norte Fluminense
Scientia Horticulturae | Year: 2016

Heterosis describes the superior performance of a heterozygous F1-hybrid in comparison with the average performance of the parental lines for a given trait in a given environment and is the result of the effects of non-additive genes. In the present study, proteins from the primary roots of the papaya (Carica papaya L.) hybrid JS12 × São Mateus and its parental inbred lines were analyzed using proteomic analyses combining the shotgun method and nanoESI-HDMSE technology. A total of 955 proteins were identified by the shotgun method, among which 261 exhibited a trend toward heterosis in the hybrid compared with the mid-parents. Non-additive proteins were divided into “above high-parent” (16.1%), “high-parent” (6.5%), “low-parent” (22.2%), and “below low-parent” (55.2%) abundance patterns. The results revealed a decrease in proteins involved in energy-consuming processes such as protein metabolism and an increase in root development proteins such as those involved in auxin polar transport and signaling regulation. The findings suggest that the hybrid possesses an optimization mechanism for protein synthesis that results in substantial improvements in cellular energy efficiency and phenotypic performance. Therefore, this study may contribute to a better understanding of the molecular basis of heterosis in papaya. © 2016 Elsevier B.V. Source

Vale E.D.M.,State University of Norte Fluminense | Heringer A.S.,State University of Norte Fluminense | Barroso T.,State University of Norte Fluminense | Ferreira A.T.D.S.,Instituto Oswaldo Cruz Instituto Oswaldo Cruz Ioc Fiocruz | And 4 more authors.
Proteome Science | Year: 2014

Background: Somatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and understanding of different biological responses. In addition, the identification of molecular markers could lead to the optimization of protocols allowing the use of biotechnology for papaya propagation and reproduction. This work aimed to investigate the effects of polyethylene glycol (PEG) on somatic embryo development and the protein expression profile during somatic embryo maturation in papaya (Carica papaya L.).Results: The maturation treatment supplemented with 6% PEG (PEG6) resulted in the greatest number of somatic embryos and induced differential protein expression compared with cultures grown under the control treatment. Among 135 spots selected for MS/MS analysis, 76 spots were successfully identified, 38 of which were common to both treatments, while 14 spots were unique to the control treatment, and 24 spots were unique to the PEG6 treatment. The identified proteins were assigned to seven categories or were unclassified. The most representative class of proteins observed in the control treatment was associated with the stress response (25.8%), while those under PEG6 treatment were carbohydrate and energy metabolism (18.4%) and the stress response (18.4%).Conclusions: The differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya. © 2014 Vale et al.; licensee BioMed Central Ltd. Source

Paiva L.B.,State University of Norte Fluminense | Correa S.F.,Federal University of Ouro Preto | Santa-Catarina C.,Laboratorio Of Biologia Celular E Tecidual | Floh E.I.S.,University of Sao Paulo | And 2 more authors.
Environmental Engineering and Management Journal | Year: 2014

Heavy metals, such as Cr (chromium), have received attention during recent years due to their disposal in soil and water. Brave peanut (Pterogyne nitens Tul.), a tropical tree, was cultivated in 500 µmol L-1 Cr+6 to observe how this species responds to this stress, with a view to its possible application in the restoration of degraded areas. In the present study, we report the use of a new method, photoacoustic spectroscopy, to evaluate the emission of CO2 and ethylene and the use of other standard techniques for assessing stress (chlorophyll a fluorescence, photosynthetic pigment determination, leaf relative water, specific leaf area, NO (nitric oxide) and polyamines. Photoacoustic spectroscopy appears to provide an innovative and efficient technique for detecting stress induced by heavy metals soon after their contact with plants. Polyamine content, as well as ethylene and NO, were seen to be sensitive to Cr+6; polyamines were decreased in leaves and increased in roots, ethylene was increased in the whole plant and NO was increased in the roots. Our results demonstrating alterations in ethylene in response to Cr+6 are, to date, unreported and the technique used for these measurements is novel. With regard to the polyamines, modulations in their concentrations may have protected the photosystem II, since no photosynthetic alterations were observed for pigments and chlorophyll a fluorescence. © Gh. Asachi Technical University of Iasi. All rights reserved. Source

Reis R.S.,State University of Norte Fluminense | Vale E.D.M.,State University of Norte Fluminense | Heringer A.S.,State University of Norte Fluminense | Santa-Catarina C.,Laboratorio Of Biologia Celular E Tecidual | Silveira V.,State University of Norte Fluminense
Journal of Proteomics | Year: 2016

Somatic embryogenesis, an important biotechnological technique, has great potential for application in sugarcane breeding and micropropagation. Polyamines have been associated with the regulation of several physiological processes, including the acquisition of embryogenic competence and somatic embryogenesis. In this study, we used a proteomic approach to evaluate the effects of exogenous polyamine on sugarcane somatic embryo development to better understand this process. Embryogenic cultures were treated with different concentrations of putrescine, spermidine, and spermine. Proteomic analyses combined the shotgun method and the nanoESI-HDMSE technology. Among polyamines, 500μM putrescine gave rise to the highest number of somatic embryos; however, no differences in the amount of fresh matter were observed between polyamines and control. Differences in protein abundance profiles resulting from the effect of 500μM putrescine on sugarcane somatic embryo maturation were observed. Proteomic analyses of putrescine and control treatment showed differences in the abundances of proteins related to somatic embryogenesis, such as arabinogalactan proteins, peroxidases, heat shock proteins, glutathione s-transferases, late embryogenesis abundant proteins, and 14-3-3 proteins. These results show that putrescine and the identified proteins play important roles in protecting the cells against an in vitro stress environment, contributing to the formation of somatic embryos during the maturation treatment. Biological significance: Despite all studies with somatic embryogenesis, the molecular mechanisms controlling the process have not been completely understood. In this study, we highlighted the effects of the polyamine putrescine on somatic embryogenesis of sugarcane and the differentially abundant proteins related to somatic embryo development. We identified six groups of important stress related proteins that are involved in the adaptation of cells to the stress environment of in vitro culture and may also be part of the mechanisms associated to the somatic embryogenesis process. Therefore, our research is trying to understand the complexity of how one single somatic cell becomes a whole plant. © 2015 Elsevier B.V. Source

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