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Arismendi-Morillo G.,Institute Investigaciones Biologicas | Hernandez I.,Institute Investigaciones Biologicas | Mengual E.,Institute Investigaciones Biologicas | Fuenmayor A.,Laboratorio Of Bacteriologia Clinica | And 2 more authors.
Arquivos de Gastroenterologia | Year: 2011

Context - The correct diagnosis and effective treatment of Helicobacter pylori gastric infection are essential in controlling this infection. Objective To compare the diagnostic value of three tests based in endoscopic gastric biopsies histopathological evaluation with hematoxylin-eosin (H-E) staining, urease rapid test and microbiological culture for detecting Helicobacter pylori active infection, in order to make recommendations for daily clinical practice. Methods Gastric biopsies from 115 adult patients (85 female/30 male) were obtained by upper gastrointestinal endoscopy and studied by histopathological evaluation with H-E (antrum-corpus), urease test in 2 hours (antrum) and microbiological culture (antrum). Results Helicobacter pylori active infection was diagnosed in 67% of patients. Helicobacter pylori active infection was detected by histopathological evaluation with H-E, urease test and microbiological culture in 87%, 79% and 70% of the positive cases, respectively. There were significant differences when histopathological evaluation with H-E and urease test rapid test when compared with microbiological test (P<0.01). There was no significant difference between histopathological evaluation with H-E and urease test (P = 0.7). The kappa index of agreement for histopathological evaluation with H-E/urease test was 0.56, histopathological evaluation with H-E/microbiological culture 0.6, and urease test/microbiological culture 0.64. Conclusions In a hospital setting like the one studied, histopathological evaluation with H-E and urease test are the most recommended tests for diagnosis of Helicobacter pylori active infection based in endoscopic biopsies. If pathological information of gastric lesions will be required, histopathological evaluation with H-E is essential. Urease test is mandatory if a prompt diagnosis is necessary. Microbiological culture can be used in cases of persistent or complicated infection, which may require studies on Helicobacter virulence or antimicrobial susceptibility. Selected cases might demand a combination of several tests. The three tests exhibit a good concordance level for Helicobacter pylori active infection diagnosis.

Arismendi-Morillo G.,University of Zulia | Hernandez I.,University of Zulia | Mengual E.,University of Zulia | Abreu N.,University of Zulia | And 4 more authors.
Revista de Gastroenterologia de Mexico | Year: 2013

Background/Aim: Severity of chronic gastritis associated with Helicobacter pylori infection (CGAHpI) could play a role in evaluating the potential risk to develop gastric cancer. Our aim was to estimate the risk for gastric cancer in a clinical setting, according to histopathologic criteria, by applying the gastric cancer risk index (GCRI) Methods: Histopathologic study of the gastric biopsies (corpus-antrum) from consecutive adult patients that underwent gastroesophageal duodenoscopy was carried out, and the GCRI was applied in patients presenting with CGAHpI. Results: One hundred eleven patients (77% female) with a mean age of 38.6±13.1 years were included. Active Helicobacter pylori infection (aHpi) was diagnosed in 77 cases (69.40%). In 45% of the cases with aHpi, pangastritis (23%) or corpus-predominant gastritis (22%) was diagnosed. Nine cases were diagnosed with intestinal metaplasia (8%), 7 of which (77.70%) were in the aHpi group. Twenty one percent of the patients with aHpi had a GCRI of 2 (18.10%) or 3 (2.50%) points (high risk index), while 79.10% accumulated a GCRI of 0 or 1 points (low risk index). Of the patients with no aHpi, none of them had 3 points (p=0.001). Of the 18 patients that accumulated 2 or 3 points, 6 (33.30%) presented with intestinal metaplasia (all with pangastritis and corpus-predominant gastritis), of which 4 cases (66.60%) had aHpi. Conclusions: The estimated gastric cancer risk in patients with CGAHpI in the clinical setting studied was relatively low and 5% of the patients had a histopathologic phenotype associated with an elevated risk for developing gastric cancer.

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