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Olivera-Castillo L.,National Polytechnic Institute of Mexico | Davalos A.,Autonomous University of Madrid | Grant G.,University of Aberdeen | Valadez-Gonzalez N.,Autonomous University of Yucatan | And 8 more authors.
PLoS ONE | Year: 2013

Sea cucumber is widely consumed as a putative functional food. It contains many biologically-active substances, but only limited research on its properties in vivo has been done. The effects of different meals containing Isostichopus badionotus, a sea cucumber from southeast Mexico, on growth performance and body lipid profile in young rats were analyzed. Sea cucumber body wall was either lyophilized, cooked (100 °C, 1 h in water) and lyophilized, or ovendried (70 °C for 12 h). It was then ground and incorporated into cholesterol- containing diets. I. badionotus meals supported growth and improved lipid profile in rats. In particular, serum cholesterol, low density lipoproteins, triglycerides concentration and atherogenic index values were greatly reduced by some I. badionotus containing diets. Liver total lipids, triglycerides and cholesterol were also reduced. Cooking or heat-treatment of the meals lowered but did not abolish their hypolipidemic potency. Gene expression analysis of several key genes involved in cholesterol and lipid metabolism in liver showed that diets containing I. badionotus repressed the induction of key genes associated with dyslipidemia exerted by cholesterol supplementation. Consumption of I. badionotus from the Yucatan Peninsula is beneficial for dyslipidemia, although biological effect is clearly dependent on preparation method. © 2013 Olivera-Castillo et al.

Menezes R.C.,Institute Nacional Of Infectologia Evandro Chagas Ini | Madeira M.F.,Laboratorio Of Vigilancia Em Leishmanioses | Ferreira L.C.,Laboratorio Of Anatomia Patologica | Barbosa Filho C.J.L.,Institute Nacional Of Infectologia Evandro Chagas Ini | And 2 more authors.
Journal of Comparative Pathology | Year: 2016

Parasitological methods are the most specific procedures used for the diagnosis of Leishmania spp. infection, but their limited sensitivity poses a disadvantage and prompts the need for alternatives. The choice of site for sample collection influences diagnostic sensitivity. The combination of an accurate diagnostic method and a technique that allows large-scale field studies is highly desirable to enhance the investigation of Leishmania spp. infection in dogs, especially in endemic regions. The bone marrow is a good target for the detection of Leishmania spp. in dogs. In this context, bone marrow aspiration is rapid and less invasive compared with biopsy procedures, and also enables cell block processing, paraffin wax embedding and the sectioning of samples for further histological and immunohistochemical analyses. The aim of this study was to describe for the first time parasitological methods (immunohistochemistry [IHC] and histopathology) using the cell block technique with bone marrow aspirates for the diagnosis of Leishmania spp. infection in dogs. Bone marrow aspiration was performed in 45 dogs from an area endemic for visceral leishmaniosis for parasitological culture and the cell block technique (histopathology and IHC). Fourteen (31.1%) dogs tested positive for Leishmania spp. by IHC, six (13.3%) by parasitological culture and four (8.9%) by histopathology. Cell block IHC was a useful tool for the diagnosis of canine visceral leishmaniosis. Further studies should be conducted to validate this method for routine epidemiological screening. © 2016 Elsevier Ltd.

Rodriguez R.,National Autonomous University of Mexico | Loske A.M.,National Autonomous University of Mexico | Fernandez F.,National Autonomous University of Mexico | Estevez M.,National Autonomous University of Mexico | And 3 more authors.
Journal of Biomaterials Science, Polymer Edition | Year: 2011

In medicine, micro-electro-mechanical systems (MEMS) perform several specific functions. The design of bio-packages for MEMS to be implanted into the human body has been an increasing challenge in the last years. Mechanical, chemical and thermal resistance, as well as excellent bonding to silicon surfaces, are needed. Furthermore, ideal bio-packages should minimize post-operative complications and be well accepted by the host. To reach this goal, two different morphology-controlled hydroxyapatite-based porous biomaterials were synthesized, implanted in rats and evaluated mechanically and histologically. The novel biomaterials were prepared at room temperature using synthetic hydroxyapatite micro-particles, silica nanoparticles and water-based resin and compared with a standard hydroxyapatite biomaterial. The morphology (porosity) was controlled to obtain interconnected pores with appropriated pore size and pore volume fraction. All biomaterials were implanted in rats at the dorsal area near the third thoracic vertebra. The rats were killed 2, 7 and 21 days after surgery. Histological analysis revealed that the implants were well accepted by the host and minimal local inflammation was observed. The acute inflammatory response disappeared 21 days after surgery for both novel biomaterials. Additionally, organic matter (collagen) was produced in the interior of the porous biomaterial, indicating that an incipient vascularization process was in progress after 21 days of implantation. Both new biomaterials showed high abrasion resistance, high Young modulus, the appropriate porosity to allow possible vascularization, and good bonding to silicon surfaces. © 2011 Koninklijke Brill NV, Leiden.

Malignant syphilis is a rare form of secondary syphilis strongly associated with human immunodeficiency virus infection (HIV). A rare clinical form of presentation include multiple, large and rupia-like ulcerative lesions associated with systemic clinical manifestations. Here we describe an HIV-seropositive female who developed clinical cutaneous and systemic manifestations of malignant rupioid syphilis, with a good response to treatment with benzatin penicillin and we performed a review of the literature.

D'Angelino R.H.R.,Instituto Biologico Of Sao Paulo Ib | Villalobos E.M.C.,Laboratorio Of Raiva E Encefalites | Gregori F.,University of Sao Paulo | Del Fava C.,Laboratorio Of Anatomia Patologica
BioMed Research International | Year: 2013

Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions. © 2013 Rubens Henrique Ramos D'Angelino et al.

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