Buenos Aires, Argentina
Buenos Aires, Argentina

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Ramirez M.S.,University of Buenos Aires | Pineiro S.,University of Maryland, Baltimore | Pineiro S.,U.S. Food and Drug Administration | Centron D.,University of Buenos Aires | And 24 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2010

In order to contribute to the knowledge of the architecture and epidemiology of class 2 integrons, we performed a class 2 integron molecular survey in which we analyzed 726 isolates in two bacterial populations from environmental and nonepidemiologically related clinical samples, respectively, collected from 1982 to 2007. We recovered the intI2 gene from 130 of 726 isolates, most of which were clinical isolates, and only 1 (a psychrophilic Pseudomonas sp.) was from a water sample. Unlike the widespread distribution of class 1 integrons within Gram-negative bacilli, only Acinetobacter baumannii and Enterobacter cloacae harbored class 2 integrons at a high frequency in our collection. Class 2 integrons with six novel cassette arrays were documented. Characterization of the transposition module of Tn7, the genetic platform in which class 2 integrons have always been reported, showed tns modules with a mosaic genetic structure. A bioinformatic analysis performed with the tns genes present in sequence databases, the finding of intI2 not associated with tns genes, and the genetic examination of novel tns-like genes found in three isolates indicated the possibility of the independent evolution of the two components related to horizontal gene transfer, the class 2 integrons and the Tn7 transposons. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Gomez-Fernandez J.A.,Laboratorio Of Analisis Clinicos | Belchior B.P.,Servicio Andaluz de Salud SAS
Revista de la Sociedad Espanola de Enfermeria Nefrologica | Year: 2010

Objective: To assess the scientific activity and information production of the journal of the Spanish Association of Renal Nursing (SEDEN) for the period 2005-2008, by means of the bibliometric study. Methods: Cross-seccional descriptive study of the results obtained from the analysis of the articles published in the journal of SEDEN. The data were obtained by consulting the electronic version through the web. All the documental possibilities were taken into account with the exception of editorials. Results: A total of 131 articles were published, 75 (57,25%) being original articles. Most of the articles are from hemodialysis area. The number of authors is 4.44 per article. The profile of authorship is an asistencial female nurse in the Catalan or Andalusian Comunity using 7,53 citations, with over 7 years old from the year of publication, for original article and quantitative methodology used. Conclusions: There is a 25% increase in the number of publications for the period 2001-2004.

Irisarri-Gutierrez M.J.,University of Valencia | Munoz-Antoli C.,University of Valencia | Acosta L.,University Miguel Hernández | Acosta L.,Laboratorio Of Analisis Clinicos | And 4 more authors.
African Health Sciences | Year: 2016

Background: Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites. Objective: To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs. Method: 497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing. Result: Hookworm-like eggs were found in 159 (32%) of the samples by Kato-Katz, finally identified as Caenorhabditis elegans by PCR technique. Conclusion: The diagnosis of human hookworm eggs, only by the use of Kato-Katz technique can lead to false positives because of similarities with eggs of other free-living worms, from wet soils like those of Rwanda that could contaminate stool samples. © 2016, Makerere University, Medical School. All rights reserved.

The introduction of automated treponemal chemiluminescence assay (CLIA) has led to a change in the diagnostic algorithm of syphilis. The objective of the work was to compare the traditional algorithm with the automated one and evaluating two CLIA assays. A total of 94 sera were tested for rapid plasma reagin (RPR), Treponema pallidum hemagglutination (TPHA) and Architect Syphil TP (Syphilis Treponema pallidum, STP). In a second phase, 100 samples were compared, STP against Immulite Syphilis screen (ISS). In the first phase, 8 false positive RPR were found with just 1 STP. In the second phase, a statistically significant difference was found in the specificity in favour of the ISS. The use of CLIA reduces analytical errors and staff time spent on the nontreponemal test. Both CLIA are useful as screening tests. © 2012 AEBM, AEFA y SEQC.

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