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Siqueira Santos R.F.,Laboratorio Of Analises Clinicas | Da Silva N.,Institute Tecnologia Of Alimentos | Amstalden Junqueira V.C.,Institute Tecnologia Of Alimentos | Kajsik M.,Nottingham Trent University | And 3 more authors.
Annals of Nutrition and Metabolism | Year: 2013

Background/Aims:Cronobacter spp. have been identified as being of considerable risk to neonates. The occurrence of organisms in infant formulas is therefore of considerable interest. Methods: The occurrence of Cronobacter spp. in infant feeds (formulas and fortified cow's milk) was determined using most probable number (MPN) analysis, and from formula preparation utensils. Ninety-nine samples were analyzed, of which 42 were unopened cans of powdered infant formula (PIF), 25 reconstituted infant formulas in feeding bottles, 27 utensils used in the preparation of infant formula and 5 samples of fortified cow's milk. Presumptive Cronobacter spp. isolates were identified using the 7 allele multilocus sequence typing (MLST) scheme. Results: C. sakazakii, C. malonaticus and C. muytjensii were recovered from PIF. Although the incidence of Cronobacter in PIF was 29% (12/42), the level was low with an average of 0.54 MPN/100 g. According to MLST profiling, C. sakazakii was the most frequently isolated Cronobacter species, and C. sakazakii ST4 (associated with neonatal meningitis) was recovered from 2/42 PIF samples at 0.51 and 0.92 MPN/100 g. Conclusions:Cronobacter spp. can be isolated from PIF and therefore strict hygienic practices during PIF preparation are important to minimize neonate exposure and reduce the risk of severe infections. Copyright © 2013 S. Karger AG, Basel.


Mieldazis S.F.A.,Federal University of ABC | Azzalis L.A.,University of Sao Paulo | Azzalis L.A.,Federal University of São Paulo | Junqueira V.B.C.,University of Sao Paulo | And 5 more authors.
Jornal de Pediatria | Year: 2010

Objectives: To determine the relationship between body mass index (BMI), homeostasis model assessment - insulin resistance (HOMA-IR) and insulinemia. Methods: This was a prospective cross-sectional observational study of 132 prepubescent schoolchildren residents in the municipality of Santo André, Brazil. Children underwent anthropometric assessment, their glycemia and insulinemia were measured and their HOMA-IR index calculated. Results: Seventy-eight of the 132 children (59.1%) were girls and 54 were boys (40.9%), with a mean age of 8.7 years and mean BMI of 13.7 kg/m2. A significant positive association was detected between HOMA-IR and BMI, insulin and BMI, weight and HOMA and between insulin and weight. It was also found that the higher the BMI, the greater the HOMA score. Conclusions: The results of this study allow for the conclusion that there is a strong association between hyperinsulinism and obesity. Measures should be taken to avoid weight gain during childhood and adolescence. Copyright © 2010 by Sociedade Brasileira de Pediatria.


Boas V.A.V.,Federal University of ABC | Rocha K.C.,University of Sao Paulo | De Oliveira C.G.B.,University of Sao Paulo | De Oliveira C.G.B.,Laboratorio Of Analises Clinicas | And 9 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

Introduction: Dengue is a public health problem in Brazil and worldwide. Objective: To determine dengue seropositivity of patients from SUS specialty laboratory by correlating the results with socio-demographic data. Methods: One hundred and eighty-four questionnaires on socio-demographic assessment and dengue transmission awareness were filled out. Enzyme linked immunosorbent assay (ELISA) method was performed for the detection of IgM and IgG antibodies against dengue virus. Results: Fifteen percent of patients had IgG against dengue virus without the presence of IgM. Conclusion: All patients showed disease and prevention awareness regardless of their socio-economic background. The asymptomatic infection must be evaluated mainly in cases of disease with fever.


Musial D.C.,Laboratorio Of Analises Clinicas | Alexandrino Becker T.C.,Laboratorio Of Analises Clinicas | Isolani A.P.,Laboratorio Of Analises Clinicas Santa Cecilia | Broetto Biazon A.C.,Laboratorio Of Analises Clinicas
Acta Scientiarum - Health Sciences | Year: 2013

To verify whether high-fat diet prepared from commercial diet plus chocolate, roasted peanuts and corn cookies induces hypercholesterolemia in mice and whether there is any hepatic involvement in this type of animal testing. Swiss mice received a high-fat diet for 15 and 30 days; plasma cholesterol, triglycerides and glucose rates were determined. Hepatic impairment was evaluated by histopathological analysis. Cholesterol levels increased 43% in animals treated with high-fat diet for 30 days. Further, histopathological analysis revealed that treatment of animals for 15 and 30 days produced hepatic steatosis and steatohepatitis, respectively. Experimental model is suitable for assessing the action of anti-hypercholesterolemia and the treatment of steatohepatitis.


PubMed | Laboratorio Of Analises Clinicas, Institute Ciencias Ambientais and Disciplina de Dermatologia
Type: Journal Article | Journal: Journal of cosmetic dermatology | Year: 2016

Acne in adult women is a frequent hard-to-manage disease with many relapse cases. It mostly interferes with the quality of life of patients, bringing them major metabolic and social losses. As androgenic hormones play a very important role in the acne pathogenesis, the early diagnosis of hyperandrogenic states is very useful for the proper evaluation of each patient and for a better choice of therapeutic management. Defining a pattern for laboratory profile analysis is important for the control of relapses of acne breakouts in adult women, which lately has been the aim of many published studies.To establish the relation between 3 alpha-diol G levels and acne in female patients with normal androgenic status without menstrual dysfunctions.The evaluation of serum 3 alpha-androstanediol glucuronide levels through an enzymatic immunoassay method (Androstanediol Glucuronide ELISA Kit) for a direct quantitative measurement in 26 patients with grade II and III acne, ages ranging from 13 to 50.Among the analyzed patients, 83% had grade II acne, and among this total, 60% were aged 14 or over. According to age, 12 studied patients showed serum 3 alpha-diol G levels within normal range and 11 patients had increased levels.A total of 60% of adult women with acne present increased levels of androgens and among those with normal levels and without menstrual dysfunctions, 50% show an increase in 3 alpha-diol G. Therefore, a pharmacological approach with anti-androgenic drugs for acne therapy in most of these patients is advisable.


Torres D.O.,Laboratorio Of Analises Clinicas
Birth defects research. Part B, Developmental and reproductive toxicology | Year: 2010

BACKGROUND: Omega-6 fatty acids are important to fetal development. However, during gestation/lactation, these fatty acids may contribute toward the development of fat tissue. Omega-9 fatty acids are associated with a reduction in serum lipids and protection from liver disease. OBJECTIVES: The present study investigated the effect of the maternal intake of omega-6 and omega-9 in hypercholesterolemic mothers on the liver of the offspring. METHODS: LDL receptor-deficient mice were fed a diet rich in either omega-6 (E6D) or omega-9 (E9D) for 45 days prior to mating and until the birth of the offspring, evaluating the effect on the offspring liver in comparison to a standard diet (STD). RESULTS: Mothers fed with the E6D experienced an increase in total cholesterol (TC) and the offspring exhibited an increase in TC, hepatic triglycerides (TG), and CC-chemokine ligand (CCL)2/monocyte chemoattractant protein (MCP)-1 as well as a reduction in HDL. Histological analysis on this group revealed steatosis, leukocyte infiltrate, and increased CCL2/MCP-1 expression. The ultrastructural analysis revealed hepatocytes with lipid droplets and myofibroblasts. The offspring of mothers fed the standard diet exhibited low serum TC, but microvesicular steatosis was observed. The offspring of mothers fed the E9D exhibited lower serum and hepatic TG as well as higher LDL in comparison to the other diets. The histological analyses revealed lower steatosis and leukocyte infiltrate. CONCLUSIONS: The findings suggest that hypercholesterolemic mothers with a diet rich in omega-6 fatty acids predispose their offspring to steatohepatitis, whereas a diet rich in omega-9 has a protective effect. 2010 Wiley-Liss, Inc.


PubMed | Laboratorio Of Analises Clinicas
Type: Journal Article | Journal: Acta medica portuguesa | Year: 2010

The genetic and epigenetic alterations are being studied as one of the causes of gastric cancer (GC) progression and development. DNA methylation is an epigenetic alteration which leads to suppressor gene silencing and proto-oncogene activation, playing an important role in carcinogenesis. The histological types of gastric carcinoma have different genetic paths and the knowledge of the molecular bases of tumoral progression leads to diagnostic accuracy and attempted therapy. CDH1 (E-cadherin) and CDKN2A (p16(INK4A)) genes are thought to be tumoral suppressor genes and PTGS2 (COX-2) and genes are involved in tumour regulation and growth. In one hand, gene silencing as an epigenetic phenomenon, and in the other hand, gene expression enhancement due to possible demethylation, simultaneously, can facilitate carcinognesis and tumoral progression. Our aim was to relate CDH1, p16(INK4A), COX-2 and EGFR genes DNA methylation with the several histological types of gastric carcinoma and chronic gastritis. We studied 55 formalin fixed paraffin embedded gastric biopsies: 35 were GC specimens (12 diffuse type, 15 intestinal type and 8 indeterminate type, according to Laurns classification) and 20 samples had chronic gastritis (CG). The DNA was treated with sodium bisulfite after extraction and then performed Methylation Specific PCR (MSP). Statistical analysis was based on chi-square test and Exact Fishers test. CpG island methylation was detected in 94% of the GC samples for CDH1, 91% for COX-2, 80% for p16(INK4A) and no methylation was detected in EGFR gene (0%). In CG, CpG island methylation was found in 100% for CDH1 and COX-2 genes, 90% for p16(INK4A) and 20% for EGFR. These results reveal significant differences in EGFR gene methylation distinguishing GC from CG (p < 0, 01), suggesting that gene demethylation leads to malignant transformation and favours the use of tyrosine-kinase inhibitors in its treatment. Genes COX2 e p16INK4A lower methylation in intestinal and diffuse types of GC, favours their different role in respective histogenesis.

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