Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg

Pedro Leopoldo, Brazil

Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg

Pedro Leopoldo, Brazil

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Pinheiro de Oliveira T.F.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | Fonseca Junior A.A.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | Camargos M.F.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | de Oliveira A.M.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | And 6 more authors.
Biologicals | Year: 2016

Although PPV has been described as a cellular contaminant, few recent studies about the presence of this virus in cell cultures, serum, and trypsin were found in the literature. The purpose of this study was to detect the presence of porcine parvovirus (PPV) by polymerase chain reaction (PCR) in cell cultures, serum, and trypsin used in official public laboratories of educational institutes and research centers. We tested samples of cell cultures (88), batches of trypsin (10), and fetal bovine serum (13) from different manufacturers. The PCR for beta-actin and GAPDH was used to evaluate the efficiency of DNA extraction from samples. The PPV DNA was detected in 52 of 88 (59.1%) cell culture samples. One in ten batches of trypsin tested for PPV DNA was positive. In no sample of fetal bovine serum, amplification of PPV DNA was observed. Positive samples were tested and confirmed by another analyst. In addition, all positive samples were sequenced. Our results indicate that regular PCR testing for PPV in cell cultures and their supplies is important. © 2015 The International Alliance for Biological Standardization.


PubMed | Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg and University of Sao Paulo
Type: Journal Article | Journal: Biologicals : journal of the International Association of Biological Standardization | Year: 2016

Although PPV has been described as a cellular contaminant, few recent studies about the presence of this virus in cell cultures, serum, and trypsin were found in the literature. The purpose of this study was to detect the presence of porcine parvovirus (PPV) by polymerase chain reaction (PCR) in cell cultures, serum, and trypsin used in official public laboratories of educational institutes and research centers. We tested samples of cell cultures (88), batches of trypsin (10), and fetal bovine serum (13) from different manufacturers. The PCR for beta-actin and GAPDH was used to evaluate the efficiency of DNA extraction from samples. The PPV DNA was detected in 52 of 88 (59.1%) cell culture samples. One in ten batches of trypsin tested for PPV DNA was positive. In no sample of fetal bovine serum, amplification of PPV DNA was observed. Positive samples were tested and confirmed by another analyst. In addition, all positive samples were sequenced. Our results indicate that regular PCR testing for PPV in cell cultures and their supplies is important.


Cunha A.L.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | Silva P.F.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | Souza E.A.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | Junior J.R.A.M.,Laboratorio Nacional Agropecuario Of Minas Gerais Lanagro Mg | And 2 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

The increasing use of antimicrobial agents such as sulfonamides by the pig industry is of concern, since residues in both pork and its by-products, when derived from animals treated improperly, can endanger human health. The aim of this study was to establish the production conditions and to evaluate the homogeneity and the stability of sulfamethazine in porcine liver quality control material, produced 'in-house' for use in ring tests of the laboratory network of residues and contaminants of the Ministry of Agriculture, Livestock and Food Supply, Brazil. In the process of preparing the material, a FOSS blender was used, where the samples were ground to obtain a homogeneous mass, which was packed in polypropylene bottles. The material resulting from this process of homogenisation was sampled and analysed by LC/MS/MS. The analytical results were statistically evaluated by one-way ANOVA. According to statistical evaluation, the material produced was considered homogeneous, with 95% confidence. Stability tests were performed with the bottles stored under the specified storage conditions. They were randomly selected and analysed in duplicate by the same analytical method as the homogeneity study. The analytical results were statistically evaluated by the procedures for a stability check described in ISO 13528:2005, indicating that the material was unstable under the conditions of storage. © 2012 Taylor & Francis.

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