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Daguer H.,Laboratorio Nacional Agropecuario LANAGRO RS | Assis M.T.Q.M.,Federal University of Santa Catarina | Bersot L.D.S.,Federal University of Parana
Ciencia Rural | Year: 2010

Meat production and consumption growth has been accompanied by a wide diversification of processed products, while the trading of non-processed meat has decreased. Phosphates and non-meat proteins, mainly soy and whey proteins, are widely used to marinate meat, improving its texture and promoting the fluids retention that can bring sensorial benefits to the consumers and improve producers' yield. On this technology, the main processes employed are the injection and the tumbling of raw materials. However, some fraudulent practices must be avoided by the meat inspection service, setting limits to the replacement of meat proteins by water and non-meat ingredients. The present article reviewed the main aspects of the use of such ingredients enlightening the importance of appropriate analytical techniques to control injected, and marinated products. Source

Hoff R.,Federal University of Rio Grande do Sul | Hoff R.,Laboratorio Nacional Agropecuario LANAGRO RS | Pizzolato T.M.,Federal University of Rio Grande do Sul | Diaz-Cruz M.S.,IDAEA
Trends in Environmental Analytical Chemistry | Year: 2016

The sulfonamides were the first class of anti-infective agents discovered in therapy for the treatment of infectious diseases, even prior to the discovery of penicillin. Since their discovery, sulfonamides (SAs) are used in the human and veterinary medicine. Through the organic waste or manure utilization or other routes as discharge of effluent wastewaters into surface water, SAs could migrate to soil and water, affecting microbiota, fauna and flora. SAs are a ubiquitous group of drugs, widely detected in environment and specific resistance genes occurrence have been correlated with SAs presence. Thus, the development of robust and reliable method for SAs monitoring is of great concern. Trace and ultra-trace levels of SAs can be detected in several matrices. In order to achieve this level of detection sample preparation strategies combined with advanced mass spectrometry techniques as triple quadrupole, linear ion trap and time of flight mass detection associated with post-run strategies were currently used to SAs monitoring. In this work, a review of the most recent published reports, including the last five years, is presented. © 2016 Elsevier B.V. Source

Hoff R.,Laboratorio Nacional Agropecuario LANAGRO RS | Hoff R.,Federal University of Rio Grande do Sul | Ribarcki F.,Laboratorio Nacional Agropecuario LANAGRO RS | Zancanaro I.,Laboratorio Nacional Agropecuario LANAGRO RS | And 5 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

Two bioactivity-based screening methods for antibiotic residue analysis (FAST Antimicrobial Screening Test and Premi®Test) were compared, in terms of sensitivity, with a new in-house developed tube test assay using Escherichia coli. Tests were performed using antibiotic standards, spiked samples and real incurred samples. The minimum inhibitory concentration (MIC) for several antibiotics was established and compared with maximum residue levels (MRLs) in samples. The results of all evaluated tests are compared with liquid chromatography-tandem mass spectrometry multi-residue screening tests to compare parameters such as sample preparation, cost, time of analysis and confidence in results. For all tests, values of half the maximum residue limit (0.5xMRL) were considered as a satisfactory target for a screening method. The potential and limitations of each method are discussed to indicate more rational and effective strategies for high-throughput residue monitoring and surveillance programmes. It was concluded that bioactivity-based screening methods are a useful tool, but the best compromise between minimum performance limits, cost and selectivity must be taken into account. For laboratories equipped with mass spectrometry, multi-class screening methods provide more specific responses with high sensitivity. © 2012 Taylor & Francis. Source

Rubensam G.,Laboratorio Nacional Agropecuario LANAGRO RS | Barreto F.,Laboratorio Nacional Agropecuario LANAGRO RS | Hoff R.B.,Laboratorio Nacional Agropecuario LANAGRO RS | Hoff R.B.,Federal University of Rio Grande do Sul | Pizzolato T.M.,Federal University of Rio Grande do Sul
Food Control | Year: 2013

A simple and inexpensive sample preparation method based on solvent extraction, followed by low temperature cleanup, was demonstrated to be applicable for the determination of avermectin and milbemycin residues in bovine muscle by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography with fluorescence (LC-FL) detection. The analytical methodology was validated according to the Commission Decision 2002/657/EC, using LC-MS/MS for confirmatory and LC-FL for quantitative purposes. Mean recovery was between 88.9 and 100.7% in three distinct concentrations. The coefficient of variation for repeatability and within-laboratory reproducibility ranged from 0.78 to 5.1% and from 0.28 to 9.0%, respectively. Method precision led to satisfactory values of decision limits (CCα) and detection capabilities (CCβ). The proposed method has been applied in the Brazilian National Residue Control Plan since 2010 for the determination of avermectins and milbemycin residues in bovine muscle samples. A total of 760 samples were analyzed and none of them presented residues at concentrations above the permitted levels established by the more recently applied directives. © 2012 Elsevier Ltd. Source

Bittencourt M.S.,Laboratorio Nacional Agropecuario LANAGRO RS | Martins M.T.,Laboratorio Nacional Agropecuario LANAGRO RS | Martins M.T.,Federal University of Rio Grande do Sul | de Albuquerque F.G.S.,Laboratorio Nacional Agropecuario LANAGRO RS | And 4 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

A multiresidue and multiclass method based on liquid chromatography-tandem mass spectrometry for the determination of antibacterials was developed and validated for screening purposes. This method can be applied to commonly used drugs in veterinary medicine such as tetracyclines, quinolones and sulfonamides. Sample preparation consists in cell disruption with sand (previously purified and washed with EDTA 100 mM) followed by protein precipitation with acidified acetonitrile. Validation was conducted in accordance to European Union requirements (2002/657/EC) for qualitative methods covering detection capability (CCβ), selectivity, specificity and stability. The method enabled the detection of 21 different drugs and had a false-compliant rate of <5% (β error) at between 25% and 50% of the maximum residue levels established by legal authorities. The methodology was successfully applied to incurred poultry samples. © 2012 Taylor & Francis. Source

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