Nicita G.,University of Carabobo |
Reigosa A.,University of Carabobo |
Torres J.,Institute Altos Estudios Dr Arnoldo Gabaldon |
Vazquez C.,University of Carabobo |
And 4 more authors.
Salus | Year: 2010
Uterine cervix cancer (UCC) constitutes a public health problem around the world. In Venezuela, it represents the second cause of death from cancer in women of reproductive age. Epidemiological studies have identified a close relationship between UCC and human papillomavirus (HPV) infection, which nowadays constitutes the most prevalent sexually transmitted disease world-wide. Previous studies have reported HPV lesions in indigenous populations of Brazilian and Bolivian South American Amazon in up to 29% of women. The aim of this study was to determine the presence of HPV infection and, at the same time, perform UCC screening through conventional cytology in Venezuela's Amazonas State. The study included 57 women from the Yekwana, Piaroa and Arawaco ethnic groups, from the High Orinoco Municipality. DNA associated to HPV was found in 35.09% of the sample studied, 45% showed DNA sequences of HPV-16 and HPV-33 (high risk), and 55% for the low-risk types (HPV-6 and HPV-11). In conclusion, a significant percentage of the indigenous population from Amazonas State in Venezuela has an elevated risk to develop UCC.
Figuera M.,Laboratorio Genomik |
Dictamen A.,Laboratorio Genomik |
Marrero C.,Laboratorio Genomik |
Borjas L.,University of Zulia |
And 2 more authors.
Forensic Science International: Genetics Supplement Series | Year: 2011
Fifteen autosomal DNA markers (D3S1358, TH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1P0, PENTA D, VWA, D8S1179, TPOX and FGA) were genotyped in a total of 1290 unrelated individuals living in Aragua (232 individuals), Carabobo (321), Táchira (431) and Zulia (306) States of Venezuela. STR amplification was carried out using GenePrint Fluorescent Systems (Promega Corp.) and the analysis of amplicons was performed on an ABI Prism™ 3130 Genetic Analyzer (Applied Biosystems). Statistical evaluations were performed using GENEPOP version 4.0 and PowerStats softwares. All markers obey Hardy-Weinberg law in the States of Aragua, Táchira, and Zulia (p>. 0.05). However, CSF1P0 marker deviates from Hardy-Weinberg expectations in Carabobo State (p= 0.029), although equilibrium could be considered after sequential Bonferroni correction. When each pair of populations is compared at the genic and genotypic level, there are no differences among Aragua, Carabobo and Zulia, but all these populations are different to Táchira population. Allele, minimum, and null allele frequencies were estimated, as well as the main forensic parameters. These are the first population studies concerning these four States, and they will contribute to the conformation of a Venezuelan national database. © 2011 Elsevier Ireland Ltd.