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Medellín, Colombia

Gaviria A.,Laboratorio Of Genetica Molecular Cruz Vital | Zambrano A.K.,Laboratorio Of Genetica Molecular Cruz Vital | Morejon G.,Laboratorio Of Genetica Molecular Cruz Vital | Galarza J.,Laboratorio Of Genetica Molecular Cruz Vital | And 4 more authors.
Forensic Science International: Genetics Supplement Series

STR loci are short, repetitive sequences (3-7 base pairs in length) distributed throughout the human genome [1]. The PowerPlex Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. Allele frequencies for 22 autosomal STRs (D3S1358, D1S1656, D2S441, D10S1248, D13S317, PENTA E, D16S539, D18S51, D2S1338, CSF1PO, PENTA D, TH01, VWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA and D22S1045) were estimated from an Ecuadorian population sample. © 2013 Published by Elsevier Ireland Ltd. Source

Xavier C.,University of Porto | Xavier C.,Innsbruck Medical University | Builes J.J.,University of Antioquia | Builes J.J.,Laboratorio Genes Ltda | And 9 more authors.

Genetic diversity of present American populations results from very complex demographic events involving different types and degrees of admixture. Through the analysis of lineage markers such as mtDNA and Y chromosome it is possible to recover the original Native American haplotypes, which remained identical since the admixture events due to the absence of recombination. However, the decrease in the effective population sizes and the consequent genetic drift effects suffered by these populations during the European colonization resulted in the loss or under-representation of a substantial fraction of the Native American lineages. In this study, we aim to clarify how the diversity and distribution of uniparental lineages vary with the different demographic characteristics (size, degree of isolation) and the different levels of admixture of extant Native groups in Colombia. We present new data resulting from the analyses of mtDNA whole control region, Y chromosome SNP haplogroups and STR haplotypes, and autosomal ancestry informative insertion-deletion polymorphisms in Colombian individuals from different ethnic and linguistic groups. The results demonstrate that populations presenting a high proportion of non-Native American ancestry have preserved nevertheless a substantial diversity of Native American lineages, for both mtDNA and Y chromosome. We suggest that, by maintaining the effective population sizes high, admixture allowed for a decrease in the effects of genetic drift due to Native population size reduction and thus resulting in an effective preservation of the Native American nonrecombining lineages. © 2015 Xavier et al. Source

Builes J.J.,Laboratorio Genes Ltda | Builes J.J.,University of Antioquia | Castro J.F.,Laboratorio Genes Ltda | Castro J.F.,University of Antioquia | And 9 more authors.
Forensic Science International: Genetics Supplement Series

Participation in interlaboratory quality control exercises is one of the main mechanisms currently used for quality assurance and continuous improvement of the trials. The objective of this study was to design, to manage and to evaluate the Colombian Exercise Interlaboratory Quality Control 2012 (CEIQC-2012). The CEIQC-2012 included both practical and theoretical exercises. For practical exercise three samples were provided, two from blood and one from buccal swab, all on FTA cards, participants were requested to process the samples according to the methods and the markers used routinely in their own laboratories. For theoretical section four exercises were sent, and only one was mandatory, the remain have different degrees of difficulty and were optional. In the mandatory exercise, the participants were asked to calculate the partial and total IP of 15 autosomal STRs markers of an alleged father and a son. This exercise involved 28 laboratories from 6 Latin American and Caribbean countries (Brazil, Ecuador, Peru, Panama, Dominican Republic and Colombia), all reported results for the theoretical mandatory and 27 for the practical. Fifty-four STR markers distributed in autosomal, Y and X chromosomes were under consensus. The Proficiency Test conducted through the Colombian National Reference Laboratory has become a useful tool for quality assurance of all Colombian laboratories and some of Latin America and Caribbean that perform DNA testing to establish biological relationships. This exercise is also an excellent opportunity for constant experts training in the region. © 2013 Elsevier Ireland Ltd. Source

Builes J.J.,Laboratorio Genes Ltda | Trejos D.,Institute Medicina Legal y Ciencias Forenses | Suarez D.,Laboratorio Of Biologia Molecular | Moreno S.,Manuela Beltran University | And 8 more authors.
Forensic Science International: Genetics Supplement Series

In 2010 QIAGEN® launched to eight kits of different combinations of STRs, including the Investigator IDplex Kit. This kit allows amplification in one PCR 16 markers. The aim of this study was to evaluate the reproducibility of Investigator IDplex Kit among Latin America laboratories. In the framework of the 'III International Theoretical-Practice Course in Populations Genetic and Biologicals Filiations' in Medellín-Colombia, all participants were invited to evaluate the reproducibility of this kit, they were provided of the necessary materials for the study. The results reported by participating were tabulated for the study the reproducibility. Results and comments were received on the agreed date of 12 of the 22 laboratories registered, one participant submits comments only. Some laboratories reported greater sensitivity Investigator IDplex Kit compared with other kits containing similar markers, also highlight the easy adaptability to existing conditions in laboratories, without involving major changes to its implementation. This paper shows the high reproducibility of Investigator IDplex Kit, a new tool offered by QIAGEN® for all laboratories that perform human identification testing and biological relationship testing using DNA markers. © 2011 Elsevier Ireland Ltd. Source

Farias D.A.B.,Central Public Health Laboratory Fortaleza | Malaga S.M.R.,State University of Ceara | Vasconcelos F.R.,State University of Ceara | Sales D.C.,Central Public Health Laboratory Fortaleza | And 6 more authors.
Forensic Science International: Genetics Supplement Series

In order to complement data obtained by other researchers who aimed to provide genetic data on the population of northeastern Brazil, the allele frequency of 15 short tandem repeats (STRs) obtained from 590 non-related individuals originating from different areas of Ceará State was determined. This group represents a hybrid population with a mixture of Native Americans, Caucasians and Africans. Our data demonstrate that the most polymorphic loci are D21S11 and Penta E. The statistical tests demonstrate that the frequency distributions of the loci analyzed are in Hardy-Weinberg equilibrium. © 2013. Source

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