Time filter

Source Type

Saavedra J.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Hernandez N.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Osses A.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Castillo A.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | And 8 more authors.
Journal of Fish Diseases | Year: 2017

Early reports accounted for two main genotypes of Piscirickettsia salmonis, a fish pathogen and causative agent of piscirickettsiosis, placing the single isolate EM-90 apart from the prototypic LF-89 and related isolates. In this study, we provide evidence that, contrary to what has been supposed, the EM-90-like isolates are highly prevalent and disseminated across Chilean marine farms. Molecular analysis of 507 P. salmonis field isolates derived from main rearing areas, diverse hosts and collected over 6 years, revealed that nearly 50% of the entire collection were indeed typed as EM-90-like. Interestingly, these isolates showed a marked host preference, being recovered exclusively from Atlantic salmon (Salmo salar) samples. Although both strains produce undistinguishable pathological outcomes, differences regarding growth kinetics and susceptibility to the antibiotics and bactericidal action of serum could be identified. In sum, our results allow to conclude that the EM-90-like isolates represent an epidemiologically relevant group in the current situation of piscirickettsiosis. Based on the consistency between genotype and phenotype exhibited by this strain, we point out the need for genotypic studies that may be as important for the Chilean salmon industry as the continuous surveillance of antimicrobial susceptibility patterns. © 2017 John Wiley & Sons Ltd.


Henriquez P.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Kaiser M.,University of Leipzig | Bohle H.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Bustos P.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile | Mancilla M.,Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile
Journal of Fish Diseases | Year: 2015

Antibiotics have been extensively used against infections produced by Piscirickettsia salmonis, a fish pathogen and causative agent of piscirickettsiosis and one of the major concerns for the Chilean salmon industry. Therefore, the emergence of resistant phenotypes is to be expected. With the aim of obtaining a landscape of the antimicrobial resistance of P. salmonis in Chile, the susceptibility profiles for quinolones, florfenicol and oxytetracycline (OTC) of 292 field isolates derived from main rearing areas, different hosts and collected over 5 years were assessed. The results allowed for the determination of epidemiological cut-off values that were used to characterize the pathogen population. This work represents the first large-scale field study addressing the antimicrobial susceptibility of P. salmonis, providing evidence of the existence of resistant types with a high incidence of resistance to quinolones. Remarkably, despite the amounts and frequency of therapies, our results disclosed that the issue of resistance to florfenicol and OTC is still in the onset. © 2015 John Wiley & Sons Ltd.

Loading Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile collaborators
Loading Laboratorio de Diagnostico y Biotecnologia ADL Diagnostic Chile Ltda Puerto Montt Chile collaborators