Perez C.,Laboratorio Clinico |
Gomez-Duarte O.G.,University of Iowa |
Arias M.L.,University of Costa Rica
American Journal of Tropical Medicine and Hygiene | Year: 2010
More than 5,000 diarrheal cases per year receive medical care at the National Children's Hospital of Costa Rica, and nearly 5% of them require hospitalization. A total of 173 Escherichia coli strains isolated from children with diarrhea were characterized at the molecular, serologic, and phenotypic level. Multiplex and duplex polymerase chain reactions were used to detect the six categories of diarrheagenic E. coli. Thirty percent (n = 52) of the strains were positive, indicating a high prevalence among the pediatric population. Enteropathogenic E. coli and enteroinvasive E. coli pathotypes were the most prevalent (21% and 19%, respectively). Pathogenic strains were distributed among the four E. coli phylogenetic groups A, B1, B2, and D, with groups A and B1 the most commonly found. This study used molecular typing to evaluate the prevalence of diarrheagenic E. coli reported in Costa Rica and demonstrated the importance of these pathotypes in the pediatric population. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.
Quiros Quiros W.,Laboratorio Clinico
Revista del Laboratorio Clinico | Year: 2013
Platelet satellitism is a phenomenon described in many medical articles, but almost exclusively associated with the adhesion of platelets to neutrophils, monocytes and basophils. Although some explanations have been proposed for this phenomenon, the underlying cause is still unknown.A case of platelet satellitism around lymphoid cells in an EDTA blood sample is reported. Analysis was performed using flow cytometry, immunohistochemistry and bone marrow biopsy, which revealed a diagnosis consistent with mantle cell lymphoma.The finding of platelet satellitism in patients with lymphoma is a very uncommon observation, but it has been reported in mantle cell lymphoma and B-cell marginal zone lymphoma. © 2011 AEBM, AEFA y SEQC.
Quesada-Lobo L.,University of Costa Rica |
Quesada-Lobo L.,Laboratorio Clinico |
Troyo A.,University of Costa Rica |
Calderon-Arguedas O.,University of Costa Rica
Biomedica | Year: 2012
Introduction. Nosocomial myiases can be an important condition from a public health perspective. However, cases of this condition reported in regional and worldwide biomedical literature are scarce. Objective. A case of nosocomial myiasis is reported from Costa Rica, where the species involved was Lucilia cuprina (Diptera: Calliphoridae). Materials and methods. A 91-year-old patient with signs of immunosuppression, severe impairment of lung function, and mechanical ventilation presented larvae in both nostrils on the seventh day after admission. Five specimens were collected and processed for identification. Results. The taxonomic identification of the specimens established that the muscoid fly species was L. cuprina. Conclusion. This is the first case of nosocomial myiasis reported from Costa Rica and in Latin America for which the etiological agent is L. cuprina.
Influence of an observer in the haemolysis produced during the extraction of blood samples in primary care [Influencia de un observador en la hemólisis producida durante las extracciones de muestras sanguíneas en atención primaria]
Bel-Pena N.,Laboratorio Clinico |
Merida-De La Torre F.J.,Laboratorio Clinico
Revista de Calidad Asistencial | Year: 2015
Objective To check whether an intervention based on direct observation and complementary information to nurses helps reduce haemolysis when drawing blood specimens. Material and methods Random sampling study in primary care centres in the serrania de Málaga health management area, using a cross-sectional, longitudinal pre- and post-intervention design. The study period was from August 2012 to January 2015. The level of free haemoglobin was measured by direct spectrophotometry in the specimens extracted. It was then checked whether the intervention influenced the level of haemolysis, and if this was maintained over time. Results The mean haemolysis measured pre-intervention was 17%, and after intervention it was 6.1%. A year later and under the same conditions, the frequency of haemolysis was measured again the samples analysed, and the percentage was 9% These results are low when compared to the level obtained pre-intervention, but are higher when compared to the levels obtained immediately after the intervention. The transport and analysis conditions were the same. Conclusions An intervention based on a direct and informative observation in the process of collecting blood samples contributes significantly to reduce the level of haemolysis. This effect is maintained in time. This intervention needs to be repeated to maintain its effectiveness. Audits and continuing education programs are useful for quality assurance procedures, and maintain the level of care needed for a good quality of care. © 2015 SECA. Published by Elsevier Espaa, S.L.U. All rights reserved.
De Carvalho J.F.,University of Sao Paulo |
De Oliveira R.M.,Laboratorio Clinico |
Rodrigues C.E.M.,University of Sao Paulo |
Glezer A.,University of Sao Paulo |
And 2 more authors.
Clinical and Developmental Immunology | Year: 2012
Objectives. The aim of this study was to investigate the HLA-G serum levels in Primary Antiphospholipid Syndrome (PAPS) patients, its impact on clinical and laboratory findings, and heparin treatment. Methods. Forty-four PAPS patients were age and gender matched with 43 controls. HLA-G serum levels were measured using an enzyme-linked immunosorbent assay (ELISA). Results. An increase in soluble HLA-G levels was found in patients compared to controls (3.35 (0-22.9) versus 1.1 (0-14), P=0.017). There were no significant differences in HLA-G levels between patients with and without obstetric events, arterial thrombosis, venous thrombosis, or stroke. Sixty-six percent of patients were being treated with heparin. Interestingly, patients treated with heparin had higher HLA-G levels than ones who were not treated with this medication (5 (0-22.9) versus 1.8 (0-16)ng/mL, P=0.038). Furthermore, patients on heparin who experienced obstetric events had a trend to increased HLA-G levels compared to patients who were not on heparin and did not have obstetric events (5.8 (0-22.9) versus 2 (0-15.2)ng/mL, P=0.05). Conclusion. This is the first study to demonstrate that serum HLA-G levels are increased in APS patients. We also demonstrated that heparin increases HLA-G levels and may increase tolerance towards autoantigens. Copyright © 2012 Jozélio Freire de Carvalho et al.