Laboratorio Clinico

Oaxaca, Mexico

Laboratorio Clinico

Oaxaca, Mexico
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Bussmann R.W.,Missouri Botanical Garden | Malca G.,Laboratorio Clinico | Glenn A.,Missouri Botanical Garden | Sharon D.,Missouri Botanical Garden | And 10 more authors.
Journal of Ethnopharmacology | Year: 2011

Ethnopharmacological relevance: The plant species reported here are traditionally used in Northern Peru for a wide range of illnesses. Most remedies are prepared as ethanol or aqueous extracts and then ingested. The aim of this study was to evaluate the potential toxicity of these extracts. Materials and methods: The toxicity of ethanolic and water extracts of 341 plant species was determined using a brine-shrimp assay. Results: Overall 24% of the species in water extract and 76% of the species in alcoholic extract showed elevated toxicity levels to brine-shrimp. Although in most cases multiple extracts of the same species showed very similar toxicity values, in some cases the toxicity of different extracts of the same species varied from non-toxic to highly toxic. Conclusions: Traditional preparation methods take different toxicity levels in aqueous and ethanol extracts into account when choosing the appropriate solvent for the preparation of a remedy. © 2011 Elsevier Ireland Ltd All rights reserved.


Bussmann R.W.,Missouri Botanical Garden | Malca-Garcia G.,Laboratorio Clinico | Glenn A.,Missouri Botanical Garden | Sharon D.,Missouri Botanical Garden | And 14 more authors.
Journal of Ethnopharmacology | Year: 2010

Aim: The plant species reported here are traditionally used in Northern Peru to treat bacterial infections, often addressed by the local healers as " inflammation" The aim of this study was to evaluate the minimum inhibitory concentration (MIC) of their antibacterial properties against Gram-positive and Gram-negative bacteria. Materials and methods: The antimicrobial activity of ethanolic and water extracts of 141 plant species was determined using a deep-well broth microdilution method on commercially available bacterial strains. Results: The ethanolic extracts of 51 species inhibited Escherichia coli, and 114 ethanolic extracts inhibited Staphylococcus aureus. In contrast, only 30 aqueous extracts showed activity against Escherichia coli and 38 extracts against Staphylococcus aureus. The MIC concentrations were mostly very high and ranged from 0.008 to 256. mg/ml, with only 36 species showing inhibitory concentrations of <4. mg/ml. The ethanolic extracts exhibited stronger activity and a much broader spectrum of action than the aqueous extracts. Hypericum laricifolium, Hura crepitans, Caesalpinia paipai, Cassia fistula, Hyptis sidifolia, Salvia sp., Banisteriopsis caapi, Miconia salicifolia and Polygonum hydropiperoides showed the lowest MIC values and would be interesting candidates for future research. Conclusions: The presence of antibacterial activity could be confirmed in most species used in traditional medicine in Peru which were assayed in this study. However, the MIC for the species employed showed a very large range, and were mostly very high. Nevertheless, traditional knowledge might provide some leads to elucidate potential candidates for future development of new antibiotic agents. © 2010 Elsevier Ireland Ltd.


Moreno-Campoy E.E.,University of Malaga | Merida-De La Torre F.J.,Laboratorio Clinico | Martos-Crespo F.,University of Malaga | Plebani M.,Hospital Of Padua
Revista de Calidad Asistencial | Year: 2015

Introduction and objective Gender is one of the factors that can influence the use of health resources. The use of tumour markers is widespread, due to the importance of these in monitoring cancer development. The aim of this study is to analyse the influence of gender on the use of tumour markers, and to investigate whether there are differences in their use. Material and methods A longitudinal, retrospective and descriptive study, with a 2-year follow-up, was conducted in the catchment area of the University Hospital of Padua. An analysis was performed on 23,059 analytical requests for tumour markers. A descriptive and frequency analysis was performed on all variables. The statistical analysis was performed using Chi squared, Student t and Mann-Whitney U to test for significance. Results The number of requests for women (1.5) was lower than men (1.6). In patients with tumour pathology, the number of requests was higher than in patients without tumour disease. In the analysis by disease and gender, the difference remained significant. As regards the number of tumour markers per request, the difference between genders was also significant: 2.13 in males versus 2.85 in women. Similar results were obtained when requests for tumour markers linked to gender-related diseases were eliminated. Conclusions There are differences in the use of tumour markers by gender with the number of requests for male patients being higher than for females. However, the number of tumour markers per request is greater in women than in men. © 2015 SECA. Published by Elsevier Espaa, S.L.U. All rights reserved.


Vieira K.F.,University of Sao Paulo | Vieira K.F.,Setor Of Imunoquimica Do Laboratorio Clinico | Shitara E.S.,University of Campinas | Shitara E.S.,Laboratorio Clinico | And 2 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

The use of quality indicators has been appreciated in laboratory management so as to optimize quality and error quantification in several laboratory processes. Furthermore, it assists in the implementation of preventive and corrective measures and it shows their corresponding efficiency. The objective of the present study is to discuss the evolution of quality, mainly in the laboratory area, focusing on the importance of quality indicators in laboratory management. Some pre-analytical, analytical and post-analytical laboratory indicators are also presented and discussed in this work. Finally, we highlight the Brazilian initiative in the Laboratory Indicator Program developed by the Brazilian Society of Clinical Pathology and Laboratory Medicine (SBPC/ML) in partnership with Control-Lab and the Model of Quality Indicator project, which has been developed by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC).


Merida De La Torre F.J.,Laboratorio Clinico | Moreno Campoy E.E.,Farmacia | Moreno Campoy E.E.,University of Malaga | Martos Crespo F.,University of Malaga
Medicina Clinica | Year: 2015

Background and objetive Improper clinical use of tumor markers (TM) may cause unnecessary additional studies to confirm or refute a positive result. After observing 2 adverse events due to a wrong use of TM, a protocol for improving their use was implemented. The objective of this study was to determine the impact of the implementation of the protocol. Material and method This was a pre-postintervention study, where analytical requests of carcinoembryonic antigen, CA15.3, CA19.9 and CA125 were analyzed during one year in patients not undergoing checking of neoplasia. A protocol was implemented and physicians were trained as recommended by the European Group on Tumor Markers, limiting its use to monitor the disease and its treatment. The study period was 2010-2014. Results The total number of requests dropped 50.81% and the percentage of adequacy of TM increased, each year, from 31.03 to 77.91%. Conclusions The implementation of a protocol for the proper use of TM contributes to a safer use, avoiding incorrect studies and unnecessary and harmful tests for the patient. © 2015 Elsevier España, S.L.U. All rights reserved.


PubMed | Hospital Of Padua, University of Malaga and Laboratorio Clinico
Type: Journal Article | Journal: Revista de calidad asistencial : organo de la Sociedad Espanola de Calidad Asistencial | Year: 2015

Gender is one of the factors that can influence the use of health resources. The use of tumour markers is widespread, due to the importance of these in monitoring cancer development. The aim of this study is to analyse the influence of gender on the use of tumour markers, and to investigate whether there are differences in their use.A longitudinal, retrospective and descriptive study, with a 2-year follow-up, was conducted in the catchment area of the University Hospital of Padua. An analysis was performed on 23,059 analytical requests for tumour markers. A descriptive and frequency analysis was performed on all variables. The statistical analysis was performed using Chi squared, Student t and Mann-Whitney U to test for significance.The number of requests for women (1.5) was lower than men (1.6). In patients with tumour pathology, the number of requests was higher than in patients without tumour disease. In the analysis by disease and gender, the difference remained significant. As regards the number of tumour markers per request, the difference between genders was also significant: 2.13 in males versus 2.85 in women. Similar results were obtained when requests for tumour markers linked to gender-related diseases were eliminated.There are differences in the use of tumour markers by gender with the number of requests for male patients being higher than for females. However, the number of tumour markers per request is greater in women than in men.


Passalacqua C.,University of Chile | Melo C.,University of Chile | Martin L.M.,University of Chile | Rojas F.,University of Chile | And 5 more authors.
American Journal of Medical Genetics, Part A | Year: 2011

Marshall-Smith Syndrome (OMIM 602535) was described initially by Marshall in two infants with a syndrome characterized by accelerated skeletal maturation, failure to thrive, and dysmorphic facial features. We report a new patient with clinical features of Marshall-Smith syndrome with additional findings such as hyperpigmented lines on trunk and the four extremities. © 2011 Wiley-Liss, Inc.


Castell Roldan E.P.,Laboratorio Clinico | Reyes Hernandez D.,Laboratorio Clinico | Reyes Gomez U.,Laboratorio Clinico | Reyes Hernandez U.,Laboratorio Clinico | And 5 more authors.
Enfermedades Infecciosas y Microbiologia | Year: 2012

INTRODUCTION. Gastroenteritis by rotavirus in infants generally come from key seats rooms of hospitals, but rarely in the community. OBJECTIVE. Rotavirus gastroenteritis cases report. MATERIAL AND METHODOS. Prospective, observational study from November 1st, 2008 to August 30, 2011.There were included newborn infants with clinically gastroenteritis and rotavirus positive stool test (rotaquick). Those with other etiologies were excluded. RESULTS. There were 12 documented cases, average age at diagnosis was 25 days, 8 newborns were exclusively breastfeeding, the median number of vomiting in 24 hours was 5, evacuations 7, cramping 8, all had abdominal distension, 10 had mild dehydration, 2 moderate dehydration, 11 were orally hydrated, 8 patients developed transiently lactose intolerance, 12 patients had successful outcomes. WBC showed bandemia leukocytosis in 4, all had lymphocytosis. Seven patients (66%) had A Rh+ blood group. Eleven showed a pH of 5 and fat + + + to + + + + in the stool. In this group mortality was 0. CONCLUSIONS. Clinical manifestations of rotavirus infection in newborn are different than that in infants. When newborns were breast-feeding the risk of worsening or death was zero. Most cases were blood group A Rh+. Rotavirus may be suspected if the stool sample have fat + + + to + + + + crosses and a pH of 5 or less.


De Carvalho J.F.,University of Sao Paulo | De Oliveira R.M.,Laboratorio Clinico | Rodrigues C.E.M.,University of Sao Paulo | Glezer A.,University of Sao Paulo | And 2 more authors.
Clinical and Developmental Immunology | Year: 2012

Objectives. The aim of this study was to investigate the HLA-G serum levels in Primary Antiphospholipid Syndrome (PAPS) patients, its impact on clinical and laboratory findings, and heparin treatment. Methods. Forty-four PAPS patients were age and gender matched with 43 controls. HLA-G serum levels were measured using an enzyme-linked immunosorbent assay (ELISA). Results. An increase in soluble HLA-G levels was found in patients compared to controls (3.35 (0-22.9) versus 1.1 (0-14), P=0.017). There were no significant differences in HLA-G levels between patients with and without obstetric events, arterial thrombosis, venous thrombosis, or stroke. Sixty-six percent of patients were being treated with heparin. Interestingly, patients treated with heparin had higher HLA-G levels than ones who were not treated with this medication (5 (0-22.9) versus 1.8 (0-16)ng/mL, P=0.038). Furthermore, patients on heparin who experienced obstetric events had a trend to increased HLA-G levels compared to patients who were not on heparin and did not have obstetric events (5.8 (0-22.9) versus 2 (0-15.2)ng/mL, P=0.05). Conclusion. This is the first study to demonstrate that serum HLA-G levels are increased in APS patients. We also demonstrated that heparin increases HLA-G levels and may increase tolerance towards autoantigens. Copyright © 2012 Jozélio Freire de Carvalho et al.


PubMed | Laboratorio Clinico
Type: Journal Article | Journal: Biomedica : revista del Instituto Nacional de Salud | Year: 2012

The hemoglobin S (HbS) mutation is accompanied by other mutations in the region of chromosome 11 known as beta globin cluster. The pattern of combination of these polymorphisms giving rise to the haplotypes that co-inherit the HbS mutation, are called haplotypes bs, and are of great epidemiological and clinical significance.The frequencies of major haplotypes associated with S beta-globin gene was determined in Colombian patients heterozygous for hemoglobin S.As part of the national neonatal screening program at Clnica Colsanitas, located in major cities of Colombia, nearly 1,200 children from different areas of the country were examined for hemoglobinopathies. The sickle cell trait was identified as the most common. S beta-globin gene haplotypes were determined by PCR and restriction enzymes in 33 children with AS hemoglobin electrophoretic patterns (carrier state). In addition, electrophoretic patterns of hemoglobin, fetal hemoglobin levels and hematologic parameters of each individual were identified.The most frequent haplotypes in Colombia were the Bant haplotype (36.4 %), followed by Senegal (30.3 %), Benin (21.2 %) and Cameroon (12.1 %) haplotypes. Hemoglobin electrophoresis confirmed the AS phenotype in all patients, and fetal hemoglobin levels below 1%. Other hematological parameters were normal in all cases.The HbS haplotypes found more frequently in the sample were of African origin, and their distribution varied according to the place of origin of the individual. The most frequent corresponded to the Bantu haplotype.

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