Laboratorio Central Of Sanidad Animal

Santa Fe de Mondújar, Spain

Laboratorio Central Of Sanidad Animal

Santa Fe de Mondújar, Spain

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Allepuz A.,Autonomous University of Barcelona | Casal J.,Autonomous University of Barcelona | Napp S.,Autonomous University of Barcelona | Saez M.,University of Girona | And 13 more authors.
Preventive Veterinary Medicine | Year: 2011

In this study we explored the spatial variation of Bovine tuberculosis (BTB) risk of being positive, new positive or persistently positive, as well as the risk of eliminating BTB in positive herds throughout Spain from 2006 to 2009 by means of hierarchical Bayesian models. The results of the models showed that the risk of infection (positive or new positive herds), persistence and elimination was lower in counties located in north and north-eastern of Spain, and in the Balearic and Canary islands than in the rest of the country. In some counties the risk of positivity was high during the four years of study, whereas there were others where the risk of positivity was high only in some of the years. With regard to the risk of persistence of BTB positive herds, counties located in the central, western and south-western part of the country had a higher risk in the three studied periods. This study has identified some specific areas of increased BTB risk in Spain, information that is useful for disease management. © 2011 Elsevier B.V.


Perez-Sancho M.,Complutense University of Madrid | Duran-Ferrer M.,Laboratorio Central Of Sanidad Animal | Garcia-Seco T.,Complutense University of Madrid | Macias P.,Laboratorio Central Of Sanidad Animal | And 9 more authors.
Veterinary Immunology and Immunopathology | Year: 2014

Antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-γ) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host-related factors, and compared with their serological response and with the basal IFN-γ responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN-γ response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38-challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-γ response was detected. This information could help to understand the interaction bacteria-host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches. © 2014 Elsevier B.V.


Checa-Moreno R.,Laboratorio Central Of Sanidad Animal | Manzano E.,University of Granada | Capitan-Vallvey L.F.,University of Granada
Analytical Methods | Year: 2014

SIMCA pattern recognition is used with amino acid chromatographic profiles in a large homemade collection of natural protein binders obtained following old recipes traditionally used by painters and considered here as the standard of classification. An initial cluster analysis of the full dataset made it possible to distinguish three main classes of protein binders: albumin, casein and collagen-like substances. An additional iterative study of each class revealed a new subclass, i.e., glair, yolk and whole egg for the albumin class; goat, sheep and cow for the casein class; and mammals and fish for the collagen class. Optimized SIMCA models for each class and subclass were obtained with good results in terms of sensitivity (90-100%), specificity (73-100%) and interclass distance (>1.4), providing identification of the protein binder present in a set of samples of different origins such as natural products, commercial binders and works of art considered cultural heritage. © 2014 the Partner Organisations.


Manzano E.,University of Granada | Rodriguez-Simon L.R.,University of Granada | Navas N.,University of Granada | Checa-Moreno R.,Laboratorio Central Of Sanidad Animal | And 2 more authors.
Talanta | Year: 2011

The correct identification of drying oils plays an essential role in providing an understanding of the conservation and deterioration of artistic materials in works of art. To this end, this work proposes the use of peak area ratios from fatty acids after ensuring that the linear responses of the detector are tested. A GC-MS method, previously reported in the literature, was revisited to its developed and validated in order to identify and quantify of eight fatty acids that are widely used as markers for drying oils in paintings, namely myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), suberic acid (2C8), azelaic acid, (2C 9) and sebacic acid (2C10). The quaternary ammonium reagent m-(trifluoromethyl)phenyltrimethylammonium hydroxide (TMTFAH) was used for derivatization prior to GC-MS analysis of the oils. MS spectra were obtained for each methyl ester derivative of the fatty acids and the characteristic fragments were identified. The method was validated in terms of calibration functions, detection and quantification limits and reproducibility using the signal recorded in SIR mode, since two of the methyl derivatives were not totally separated in the chromatographic run. The proposed method was successfully applied to identify and characterise the most widely used drying oils (linseed oil, poppy seed oil and walnut oil) in the painting La Encarnación. This 17th century easel painting is located in the main chapel of the cathedral in Granada (Spain) and was painted by the well-known artist of the Spanish Golden Age, Alonso Cano (1601-1667). © 2011 Elsevier B.V. All rights reserved.


Crespo Leon F.,Instituto Murciano Of Investigacion Y Desarrollo Agrario Y Alimentario Imida | Saez Llorente J.L.,Subdireccion General de Sanidad e Higiene Animal y Trazabilidad | Reviriego Gordejo F.J.,European Commission | Rodriguez Ferri E.F.,University of León | Duran Ferrer M.,Laboratorio Central Of Sanidad Animal
OIE Revue Scientifique et Technique | Year: 2012

Caprine and ovine brucellosis is one of the most serious and complex animal health problems faced by Veterinary Services in countries where the disease is endemic. Various geographical factors and the nature of the disease itself influence its epidemiology, encouraging widespread distribution and, at the same time, impeding the ability of animal health programmes to prevent, control and eradicate it. Although strategies against brucellosis have traditionally been based on two specific tools (namely, vaccination of the at-risk population and testing and slaughter of animals which are suspected of or test positive for the disease), other complementary tools of a technical or administrative nature should also be considered. Experience in the European Union has shown that these tools are necessary to guarantee sustainable progress and success against this disease. However, these complementary tools have not always received sufficient attention during the strategic planning and subsequent implementation of animal health programmes, with consequent reductions in efficiency. The aim of this article is to review these complementary tools, in order to facilitate their adoption and use by official Veterinary Services, according to the resources available.


Carrera-Gonzalez M.P.,University of Jaén | Ramirez-Exposito M.J.,University of Jaén | Mayas M.D.,University of Jaén | Garcia M.J.,Laboratorio Central Of Sanidad Animal | Martinez-Martos J.M.,University of Jaén
Life Sciences | Year: 2013

An association between breast cancer and thyroid dysfunction exists although the underlying mechanisms remain to be elucidated. Numerous studies have characterized the role of thyroid hormones in controlling the synthesis and secretion of renin-angiotensin system (RAS) components, but little information is available on the putative role of the local RAS on thyroid function. Aims: Here we analyze several soluble and membrane-bound RAS-regulating aminopeptidase activities in thyroid gland from rats with mammary tumors and the relationship with the circulating levels of thyroid stimulating hormone (TSH) and free thyroxin (fT4). Main methods: We analyze soluble and membrane-bound RAS-regulating aminopeptidase activities fluorometrically using their corresponding aminoacyl-β-naphthylamide as the substrate. Key findings We have found in rats with mammary tumors a concomitant change of thyroid RAS-regulating enzymes and thyroid hormone production. Significance: We suggest that existence of alterations in the regulatory mechanisms mediated by the angiotensins of the local tissue RAS as a consequence of the carcinogenic process which could act alone or in combination with alterations at a higher level of regulation such as the hypothalamus-pituitary axis. © 2013 Elsevier Inc. All rights reserved.


Del Pilar Carrera M.,University of Jaén | Ramirez-Exposito M.J.,University of Jaén | Mayas M.D.,Hospital Carlos Haya | Garcia M.J.,Laboratorio Central Of Sanidad Animal | Martinez-Martos J.M.,University of Jaén
Tumor Biology | Year: 2010

Angiotensin II in particular and/or the local renin-angiotensin system in general could have an important role in epithelial tissue growth and modelling; therefore, it is possible that it may be involved in breast cancer. In this sense, previous works of our group showed a predominating role of angiotensin II in tumoral tissue obtained from women with breast cancer. However, although classically angiotensin II has been considered the main effector peptide of the renin-angiotensin system cascade, several of its catabolism products such as angiotensin III and angiotensin IV also possess biological functions. These peptides are formed through the activity of several proteolytic regulatory enzymes of the aminopeptidase type, also called angiotensinases. The aim of this work was to analyse several specific angiotensinase activities involved in the renin-angiotensin system cascade in mammary tissue from control rats and from rats with mammary tumours induced by N-methyl-nitrosourea (NMU), which may reflect the functional status of their target peptides under the specific conditions brought about by the tumoural process. The results show that soluble and membrane-bound specific aspartyl aminopeptidase activities and membrane-bound glutamyl aminopeptidase activity increased in mammary tissue from NMU-treated animals and soluble aminopeptidase N and aminopeptidase B activities significantly decreased in mammary tissue from NMU-treated rats. These changes support the existence of a local mammary renin-angiotensin system and that this system and its putative functions in breast tissue could be altered by the tumour process, in which we suggest a predominant role of angiotensin III. All described data about the renin-angiotensin system in mammary tissue support the idea that it must be involved in normal breast tissue functions, and its disruption could be involved in one or more steps of the carcinogenesis process. © 2010 International Society of Oncology and BioMarkers (ISOBM).


Ruiz-Sanjuan M.D.,University of Jaén | Martinez-Martos J.M.,University of Jaén | Carrera-Gonzalez M.P.,University of Jaén | Mayas M.D.,University of Jaén | And 3 more authors.
Integrative Cancer Therapies | Year: 2015

Hypothesis. Renin-angiotensin system (RAS) has been considered not only as a regulator of systemic volume and electrolyte balance but also has been recently involved in various pathological processes such as cancer. In the etiology of breast cancer, dietary factors have been analyzed and especially the influence of dietary fat has been studied, but the underlying mechanisms remain unclear. In this study, we analyzed RAS-regulating enzymes in serum of rats with N-methyl nitrosourea (NMU)-induced breast cancer fed with different diets. Study Design. Four groups of rats were injected intraperitoneally with 3 doses of 50 mg/kg body weight of NMU at different days after birth and were fed with an AIN-93 commercial diet or AIN-93 diets with 4% fat constituted respectively by extra virgin olive oil, refined sunflower oil, and refined sunflower oil enriched to 50% with oleic acid. Method. After sacrifice, blood and tumor samples were collected by spectrophotometric determinations of RAS-regulating enzymes in plasma and histopathology studies. Results. We show that the type of dietary fat does not influence latency period, incidence of animals with tumors, incidence of mortality, or tumor yield per rat. However, changes were observed in tumor volume and the histopathology. The type of dietary fat also differently modified the enzymes involved in RAS regulation. Conclusions. It might suggest that one of the mechanisms by which dietary fat affects breast cancer is the modification of the RAS system, which may be consider as a new target for integrative therapies. © The Author(s) 2014.


PubMed | University of Jaén and Laboratorio Central Of Sanidad Animal
Type: Journal Article | Journal: Integrative cancer therapies | Year: 2015

Renin-angiotensin system (RAS) has been considered not only as a regulator of systemic volume and electrolyte balance but also has been recently involved in various pathological processes such as cancer. In the etiology of breast cancer, dietary factors have been analyzed and especially the influence of dietary fat has been studied, but the underlying mechanisms remain unclear. In this study, we analyzed RAS-regulating enzymes in serum of rats with N-methyl nitrosourea (NMU)-induced breast cancer fed with different diets.Four groups of rats were injected intraperitoneally with 3 doses of 50 mg/kg body weight of NMU at different days after birth and were fed with an AIN-93 commercial diet or AIN-93 diets with 4% fat constituted respectively by extra virgin olive oil, refined sunflower oil, and refined sunflower oil enriched to 50% with oleic acid.After sacrifice, blood and tumor samples were collected by spectrophotometric determinations of RAS-regulating enzymes in plasma and histopathology studies.We show that the type of dietary fat does not influence latency period, incidence of animals with tumors, incidence of mortality, or tumor yield per rat. However, changes were observed in tumor volume and the histopathology. The type of dietary fat also differently modified the enzymes involved in RAS regulation.It might suggest that one of the mechanisms by which dietary fat affects breast cancer is the modification of the RAS system, which may be consider as a new target for integrative therapies.

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