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Ciudad del Este, Paraguay

de Mendonca M.C.L.,University Of Passo Fundo | de Amorim Ferreira A.M.,University Of Passo Fundo | dos Santos M.G.M.,University Of Passo Fundo | Oviedo E.C.,Laboratorio Central Of Salud Publica | And 5 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2011

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

Canteros C.E.,ANLIS Dr. Carlos G. Malbran | Toranzo A.,ANLIS Dr. Carlos G. Malbran | Ibarra-Camou B.,ANLIS Dr. Carlos G. Malbran | David V.,Hospital Interzonal San Juan Bautista | And 23 more authors.
Revista Argentina de Microbiologia | Year: 2010

Clinical cases of coccidioidomycosis are rare in Argentina and are generally found in the large arid precordilleran area of the country. This study aims to perform a retrospective review of all coccidioidomycosis cases documented in the country from 1892 to 2009, and to describe those occurring in the last 4 years. One hundred and twenty eight cases were documented in the 117 year-period. Since the original description of the disease in 1892 until 1939, only 6 cases were registered; between 1940 and 1999, 59 (6-14/10 yrs) and the remaining 63 (49% of total cases) occurred in the last decade. The median age of 34 patients registered in 2006-2009 was 31 years (range: 7-89), male/female ratio was 1.3:1 and 12 patients were immunocompromised. Twenty-six cases were confirmed by direct microscopy and/or culture whereas the remaining ones by serology. All isolates were identified as Coccidioides posadasii. Thirty patients lived in a vast geographic region with epicenter in Catamarca Valley. Between 2006 and 2009, annual disease incidence rates in Catamarca Province increased from historical values below 0.5/100,000 to 2/100,000 inhabitants. Such increase suggests an emergency of coccidioidomycosis in that region.

Weiler N.,Laboratorio Central Of Salud Publica | Leotta G.A.,Instituto Nacional Of Enfermedades Infecciosas | Leotta G.A.,CONICET | Zarate M.N.,Laboratorio Central Of Salud Publica | And 3 more authors.
Revista Argentina de Microbiologia | Year: 2011

During March 2007 there was an epidemic outbreak associated with the consumption of ultrapasteurized milk. Four hundred people were affected and 60 required hospitalization. S. aureus subspecies aureus was isolated from 5 patients, 3 operators and 3 milk samples. All strains produced enterotoxins. Strains isolated from 3 patients, one operator and all the milk samples carried the genes encoding enterotoxins C (sec) and D (sed), and showed an indistinguishable macrorestriction pattern (Smal-PFGE). Milk was identified as the source of intoxication and a production line operator as the source of contamination. This is the first foodborne outbreak reported in Paraguay whose agent was isolated, characterized and subtypified in the production plant, the food and the affected people.

Khan M.A.,Erasmus University Rotterdam | Northwood J.B.,Quotient Bioresearch Ltd. | Loor R.G.J.,Erasmus University Rotterdam | Tholen A.T.R.,Erasmus University Rotterdam | And 6 more authors.
Clinical Microbiology and Infection | Year: 2010

Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element. © 2009 The Authors. Journal Compilation.

Durand L.O.,Centers for Disease Control and Prevention | Cheng P..-Y.,Centers for Disease Control and Prevention | Palekar R.,Pan American Health Organization | Clara W.,Centers for Disease Control and Prevention | And 17 more authors.
Influenza and other Respiratory Viruses | Year: 2016

Background: Influenza-associated illness results in increased morbidity and mortality in the Americas. These effects can be mitigated with an appropriately chosen and timed influenza vaccination campaign. To provide guidance in choosing the most suitable vaccine formulation and timing of administration, it is necessary to understand the timing of influenza seasonal epidemics. Objectives: Our main objective was to determine whether influenza occurs in seasonal patterns in the American tropics and when these patterns occurred. Methods: Publicly available, monthly seasonal influenza data from the Pan American Health Organization and WHO, from countries in the American tropics, were obtained during 2002-2008 and 2011-2014 (excluding unseasonal pandemic activity during 2009-2010). For each country, we calculated the monthly proportion of samples that tested positive for influenza. We applied the monthly proportion data to a logistic regression model for each country. Results: We analyzed 2002-2008 and 2011-2014 influenza surveillance data from the American tropics and identified 13 (81%) of 16 countries with influenza epidemics that, on average, started during May and lasted 4 months. Conclusions: The majority of countries in the American tropics have seasonal epidemics that start in May. Officials in these countries should consider the impact of vaccinating persons during April with the Southern Hemisphere formulation. © 2016 John Wiley & Sons Ltd.

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