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Dallagassa C.B.,Federal University of Parana | Huergo L.F.,Federal University of Parana | Stets M.I.,Federal University of Parana | Pedrosa F.O.,Federal University of Parana | And 9 more authors.
Genetics and Molecular Research | Year: 2014

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions. © FUNPEC-RP.

Vidal L.R.R.,Federal University of Parana | de Almeida S.M.,Federal University of Parana | de Almeida S.M.,Pele Pequeno Principe Research Institute | de Messias-Reason I.J.,Federal University of Parana | And 8 more authors.
Arquivos de Neuro-Psiquiatria | Year: 2011

Viral meningitis is a common infectious disease of the central nervous system (CNS) that occurs worldwide. The aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in Curitiba, PR, Brazil. During the period of July 2005 to December 2006, 460 cerebrospinal fluid (CSF) samples with lymphomonocytary meningitis were analyzed by PCR methodologies. Fifty nine (12.8%) samples were positive. Enteroviruses was present in 49 (83%) samples and herpes virus family in 10 (17%), of these 6 (10%) herpes simplex virus, 1 (2%) Epstein Barr virus, 2 (3%) human herpes virus type 6 and 1 (2%) mixed infection of enterovirus and Epstein Barr virus. As conclusion enterovirus was the most frequent virus, with circulation during summer and was observed with higher frequency between 4 to 17 years of age. PCR methodology is an important method for rapid detection of RNA enterovirus and DNA herpesvirus in CSF.

De Almeida Torres R.S.L.,Laboratorio Of Bacteriologia | De Almeida Torres R.S.L.,Federal University of Parana | De Almeida Torres R.S.L.,Positivo University | De Almeida Torres R.S.L.,Laboratorio Central do Estado do Parana | And 6 more authors.
Microbial Drug Resistance | Year: 2011

Background: Scarce data are available about the antimicrobial resistance of Group A Streptococcus in South America. Methods: This study evaluated the antimicrobial susceptibility profile of 1,112 isolates of Group A Streptococcus during the period from 1993 to 2009 in Curitiba city, Brazil. Macrolide-resistant isolates were characterized by emm typing and pulsed-field gel electrophoresis. Results: All isolates were susceptible to penicillin, vancomycin, and tigecycline. On the contrary, 18.6% of the isolates were resistant to tetracycline, presenting a minimum inhibitory concentration (MIC)50/MIC90 of 32/64 mg/L. Erythromycin resistance rose from 1.9% before 2000 to 4% after 2000 and was associated with a marked increased of MIC levels. Simultaneously, both the phenotype and genotype of macrolide resistance were modified as the M phenotypes (mef(A) genotype) were replaced by the cMLSB phenotypes (erm(B) genotype). Conclusion: This polyclonal spreading of cMLSB macrolide resistance has not been previously observed in South America and should stimulate further epidemiological surveillance in this part of the world. © 2011, Mary Ann Liebert, Inc.

Assis F.E.A.,Federal University of Parana | Wolf S.,Federal University of Parana | Surek M.,Federal University of Parana | De Toni F.,Laboratorio Municipal Of Curitiba | And 5 more authors.
Journal of Infection in Developing Countries | Year: 2014

Introduction: A wide diversity of bacterial agents may cause diarrhea, presenting challenges to clinical laboratories to define a diagnosis. Considering that most stool cultures are negative, we screened stool samples from patients with diarrhea for the presence of 14 bacterial enteropathogens, aiming to establish which of them should be included in routine stool analysis.Methodology: Stool samples from 400 patients with diarrhea were analyzed for the presence of Salmonella, Shigella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Vibrio, Yersinia enterocolitica, and diarrheagenic Escherichia coli using conventional microbiological methods and PCR. Two distinct samples were studied; one included predominantly patients involved in outbreaks, and the other patients of low socioeconomic status presenting sporadic cases of diarrhea.Results: In total, 86 cultures (21.5%) were positive. Mixed infections were found in five patients, leading to recovery of 91 strains of enteropathogenic bacteria: Salmonella Enteritidis (9.2%), Aeromonas (7.2%), diarrheagenic E. coli (5.2%), and C. jejuni (1%). However, Salmonella predominated, with 11.5% frequency in diarrhea outbreaks, while Aeromonas predominated among patients of lowsocioeconomic status, with 14.6% frequency.Conclusion: Aeromonas and diarrheagenic E. coli, which are not routinely screened for, deserve to be included in laboratory screening panels. © 2014 Assis et al.

Benetti T.M.,Federal University of Parana | Monteiro C.L.B.,Federal University of Parana | Beux M.R.,Federal University of Parana | Abrahao W.M.,Federal University of Parana | Abrahao W.M.,Laboratorio Central do Estado do Parana
Brazilian Journal of Microbiology | Year: 2013

This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method. © 2013, Sociedade Brasileira de Microbiologia.

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