Costa E.O.A.,Pontifical Catholic University of Goias |
Silva D.D.M.E.,Pontifical Catholic University of Goias |
Silva D.D.M.E.,Setor Universitario |
Melo A.V.D.,Pontifical Catholic University of Goias |
And 8 more authors.
Mutagenesis | Year: 2011
A serious radiological accident occurred in 1987 in Goiânia, Brazil, which lead to extensive human and environmental contamination as a result of ionising radiation (IR) from caesium-137. Among the exposed were those in direct contact with caesium-137, their relatives, neighbours, liquidators and health personnel involved in the handling of the radioactive material and the clean-up of the radioactive sites. The exposed group consisted of 10 two-generation families, totalling 34 people. For each exposed family, at least one of the progenitors was directly exposed to very low doses of γ-IR. The control group consisted of 215 non-irradiated families, composed of a father, mother and child, all of them from Goiânia, Brazil. Genomic DNA was purified using 100 μl of whole blood. The amplification reactions were prepared according to PowerPlex® 16, following the manufacturer's instructions. Genetic profiles were obtained from a single polymerase chain reaction amplification. The exposed group had only one germline mutation of a paternal origin in the 'locus' D8S1179 and the observed mutation presented a gain of only one repeat unit. In the control group, 11 mutations were observed and the mutational events were distributed in five loci D16S539, D3S1358, FGA, Penta E and D21S11. The mutation rates for the exposed and control groups were 0.006 and 0.002, respectively. There was no statistically significant difference (P = 0.09) between the mutation rate of the exposed and control groups. In conclusion, the quantification of mutational events in short tandem repeats can provide a useful system for detecting induced mutations in a relatively small population. © The Author 2011. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. Source
Vieira T.C.,Federal University of Goais |
Vieira T.C.,Laboratorio Of Citogenetica Humana E Genetica Molecular |
Vieira T.C.,Pontifical Catholic University of Goias |
Vieira T.C.,State University of Goias |
And 9 more authors.
Genetics and Molecular Research | Year: 2014
The central region of Brazil was colonized by internal migration of individuals of different origins, who contributed to the genetic diversity existing in this population. This study determined the allele frequencies and haplotype diversity of Y-STRs in Goiás State, Central Brazil, and compared the data obtained with a sample of the Brazilian population, consisting of individuals from the five geographical regions of Brazil. A total of 353 males were typed for 12 Y-chromosome short tandem repeat (Y-STR) markers. We selected males who had no degree of relatedness, from the five mesoregions of Goiás State. DNA was extracted from blood samples followed by the amplification of the 12 Y-chromosome loci. The products were analyzed to obtain the allele profiles on an ABI3500 automated sequencer using the Gene Mapper software. Allele frequencies and haplotype diversity were estimated by direct counting, and gene diversity for each locus was computed using the Arlequin software. The results are consistent with the history of miscegenation of the population of Central Brazil, in which we observed 321 different haplotypes. The average gene diversity at the 12 loci was 0.645. DYS385b and DYS389I showed the highest (0.704) and lowest (0.520) genetic diversity values, respectively. The FST value between the Brazilian and Goiás populations was 0.00951, showing no statistical significance. The results of this study allowed the establishment of haplotypes found in the forensic samples of Goiás State serving as a reference in the elucidation of criminal cases and paternity tests, as well as population and evolutionary inferences. © FUNPEC-RP. Source
Silva M.B.,University Catolica Of Goias |
Silva D.D.M.E.,University Catolica Of Goias |
Silva D.D.M.E.,Laboratorio Of Citogenetica Humana E Genetica Molecular |
Rodovalho R.G.,Laboratorio Biocroma |
And 4 more authors.
Forensic Science International: Genetics | Year: 2010
Allele frequencies for 15 short tandem repeats included in Powerplex 16 Kit (Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818) were determined in a sample of 429 unrelated individuals from the population of Goiânia, Goias, Central Brazil. Determination of the allele frequencies as well as of several commonly used statistics in forensic and paternity testing were defined. The forensic parameters presented high values and the most polymorphic loci were Penta E, following FGA and D18S51. The exact test demonstrated that the fifteen loci analyzed in the population of Goiania have no deviation from Hardy-Weinberg equilibrium (P > 0.05). © 2009 Elsevier Ireland Ltd. All rights reserved. Source