de Oca-Porto M.R.,Laboratorio Antidoping
Ciencias Marinas | Year: 2011
The presence of petrogenic hydrocarbons was evaluated in surface sediments from the northeastern Havana littoral (Cuba). Different geochemical biomarkers were used to differentiate between biogenic and anthropogenic hydrocarbons. Sediment samples were Soxhlet extracted and analyzed by gas chromatography with flame ionization and mass spectrometer detectors. Total concentrations of aliphatic hydrocarbons ranged from 5.5 ± 0.3 to 225.7 ± 12.6 μg g-1. The presence of an unresolved complex mixture, and the detection of hopanes and of the isoprenoid compounds pristane and phytane revealed the occurrence of petroleum-derived hydrocarbons in the sediment samples.
Strano-Rossi S.,Laboratorio Antidoping |
Bermejo A.M.,Institute of Legal Medicine |
De La Torre X.,Laboratorio Antidoping |
Botre F.,Laboratorio Antidoping |
Botre F.,University of Rome La Sapienza
Analytical and Bioanalytical Chemistry | Year: 2011
A fast gas chromatography (GC)-MS method has been developed and validated for the simultaneous screening of different classes of drugs of abuse in urine. Tetrahydrocannabinol metabolite, cocaine, opiates such as morphine, O-6-monoacetylmorphine (O-6-MAM), codeine, opioids such as buprenorphine, methadone, pentazocine, fentanyl and analogues and their main metabolites can be detected and quantified after a simple liquid-liquid extraction in alkaline conditions and derivatisation to obtain the corresponding trimethylsilyl derivatives. The chromatographic separation is performed in a total time of 6 min, using a short GC column (5% phenyl methyl silicone, 10-m length∈×∈0.18-mm internal diameter). The Limits of Detection are satisfactory for forensic purposes for all the substances; the repeatability of concentrations (percent coefficients of variation) are always lower than 15% at high and low concentration levels, and accuracy, intended as % error on the true value, is always lower than 15% for all the analytes. The method can successfully be applied for screening analyses in many fields of forensic toxicology. © 2010 Springer-Verlag.