Laboratories and Animal Research Center

Muscat, Oman

Laboratories and Animal Research Center

Muscat, Oman
SEARCH FILTERS
Time filter
Source Type

Tageldin M.H.,Sultan Qaboos University | Damir H.A.,PO Box 85689 | Omer E.A.,Veterinary Laboratory | Ali M.A.,United Arab Emirates University | Adam A.M.,Laboratories and Animal Research Center
Comparative Clinical Pathology | Year: 2016

In humans, spindle cell lesions associated with thyroid adenoma or carcinoma are rare. A cross section of the thyroid of a dromedary camel showed follicular cysts, and microscopically, both thyroid lobes showed colloid goitre. The left lobe showed spindle cell proliferation associated with follicular adenoma without evidence of capsular or vascular invasion. The spindle cell lesion had cells featuring ground glass or pseudoinclusion nuclei and showed extensive haemorrhage, hyalinization and thrombosis. The right thyroid lobe showed papillary adenoma. Immunohistochemistry showed negative expression for malignancy. Serum Se, free tri-iodothyronine (fT3) and free thyroxine (fT4) values were low and CK activity was high. The histological features were unusual and merit reporting. © 2015, Springer-Verlag London.


Manjunatha B.M.,Laboratories and Animal Research Center | David C.G.,Indian National Institute of Animal Nutrition and Physiology | Pratap N.,Laboratories and Animal Research Center | Al-Bulushi S.,Laboratories and Animal Research Center | Hago B.E.,Laboratories and Animal Research Center
Animal Reproduction Science | Year: 2012

The present study was carried out to elucidate the effect of progesterone (P4) from the induced corpus luteum (CL) on the characteristics of the dominant follicle (DF) in dromedary camels (Camelus dromedarius). Ovarian follicular and induced CL dynamics were monitored by transrectal ultrasonography in eight camels during the peak breeding season. The characteristics of the DF were monitored daily from the day of emergence into a wave, until it appeared to lose its dominance and the DF of a subsequent wave grew to a diameter of 13-17. mm. At this stage ovulation was induced by hCG and the DF was monitored every 8. h for 48. h. After ovulation, CL dynamics and follicular development (emergence of a new wave, growth and mature phase of the selected DF) were monitored daily. Blood samples were collected during each ultrasound examination to study the P4 profile in these animals. The CL developed to a maximum size (22.55 ± 3.24. mm) with a peak concentration of P4 (4.60 ± 2.57. ng/ml) 7 days after ovulation. The size of the CL was positively correlated with the P4 concentration (r= 0.612) during the different stages of the CL dynamics. The presence of CL did not affect the linear growth rate, duration of growth and mature phases of the DF. The development of the DF to its maximum size during its mature phase and inter-wave interval were not affected by the P4 secreted by the induced CL. In conclusion, there is no evidence from this study to suggest that P4 from induced CL altered the characteristics of a DF in dromedary camels. © 2012 Elsevier B.V.


Manjunatha B.M.,Laboratories and Animal Research Center | Al-Bulushi S.,Laboratories and Animal Research Center | Pratap N.,Laboratories and Animal Research Center
Animal Reproduction Science | Year: 2015

This study was conducted to develop a hormone protocol that precisely synchronises follicular development for a timed breeding (TB) programme in dromedary camels. To examine the effect of GnRH treatment at four known stages of follicular development, animals were treated with GnRH when the largest follicle of the wave was 4-7, 8-11, 12-17 and 18-27mm in diameter. Transrectal ultrasonography was carried out daily up to 20 days after treatment. A hormone protocol (FWsynch) for the synchronisation of follicular wave and TB consisting of GnRH-1 (GnRH) on Day 0, PG-1 (PGF) on Day 7, GnRH-2 on Day 10 and PG-2 on Day 17 was initiated at four known stages of follicular development. Ovarian structures were monitored by ultrasonography. The FWsynch protocol was initiated at random stages of follicle development and animals were bred by natural mating at a fixed time at the research facility and in field. The pregnancy was diagnosed by ultrasonography. GnRH treatment in animals with a dominant follicle (DF) of ≥11mm in diameter resulted in synchronous new follicular wave emergence, whereas in animals with a DF≤10mm, the treatment did not alter the development of the existing follicular wave. The FWsynch protocol was effective in synchronising the follicular wave for TB irrespective of the stage of follicular development at the beginning of the protocol. TB using FWsynch protocol resulted in a pregnancy rate of 60.2% in a research facility and 53.6% and 45.6% in normal and infertile camels respectively under field conditions. © 2015 Elsevier B.V.


Manjunatha B.M.,Laboratories and Animal Research Center | Al-Bulushi S.,Laboratories and Animal Research Center | Pratap N.,Laboratories and Animal Research Center
Reproductive Biology | Year: 2015

The acquisition of ovulatory capacity in the growing dominant follicle (DF) of dromedary camels was examined in the current study. Ovulation occurred in response to hCG (1500. IU) in 27.3%, 58.3% or 100% of camels with follicles of 9, 10 or 11. mm diameter, respectively. A high dose of hCG (4500. IU) resulted in ovulation of 77.8% and 100% of camels with follicles of 9 and 10. mm, respectively. In naturally mated animals, ovulation occurred in 36.4% and 92.8% of camels with 10 and 11. mm follicles, respectively. © 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.


Manjunatha B.M.,Laboratories and Animal Research Center | Pratap N.,Laboratories and Animal Research Center | Al-Bulushi S.,Laboratories and Animal Research Center | Hago B.E.,Laboratories and Animal Research Center
Theriogenology | Year: 2012

Ovarian follicular dynamics was monitored by transrectal ultrasonography, for a period of 60 to 90 days, and its correlation with plasma estradiol-17β (E2) and progesterone (P4) were studied in seventeen, multiparous, non-lactating, 12 to 20-year-old dromedary camels. The average number of follicles recruited (12.77 ± 0.93) in each wave between animals varied (P < 0.001). The number of follicles recruited during different follicular waves was highly repeatable (0.95) within individual animals. The growth and mature phase periods of the dominant follicle (DF) were 6.10 ± 0.15 and 10.20 ± 0.47 days, respectively with a linear growth rate of 1.17 ± 0.02 mm/day between Day 0 and 10 of the follicular wave. There was an inverse relationship between the diameter of the largest DF and number of follicles (r = -0.95, P < 0.001). The DF development did not regularly alternate between the ovaries and the incidence of codominance was 45%. The mean maximum diameter of DF during its mature phase was 27.30 ± 0.78 mm and oversized follicle was 38.43 ± 1.41 mm. In 73.3% waves, the DF continued its growth for a period of 10.64 ± 1.53 days even after losing its dominance and developed into oversized follicle. The duration of the regression phase of DF and oversized follicle were 24.71 ± 3.79 and 18.50 ± 2.23 days. The mean duration of a complete follicular wave was 47.11 ± 2.94 days with an interwave interval (IWI) of 16.36 ± 0.37 days. The IWI within an individual was repeatable (0.88) and between the animals was variable (P < 0.001). Plasma E2 concentration profiles showed a wave like pattern. The peak plasma E2 concentrations were attained approximately 12 days after beginning of the growth phase, when the largest DF grew to a diameter of 18.7 mm. Plasma concentration of P4 was below 1.0 ng/mL in 85% of waves and above 1.0 ng/mL in 15% of the waves for a period of 3 to 6 days in the absence of spontaneous ovulation. It is concluded that ovarian follicular development and plasma E2 concentrations occurs in a wave like pattern in dromedary camels and the IWI and follicle numbers recruited per wave are variable between the animals and repeatable within an individual animal. © 2012 Elsevier Inc.


Manjunatha B.M.,Laboratories and Animal Research Center | Al-Bulushi S.,Laboratories and Animal Research Center | Pratap N.,Laboratories and Animal Research Center
Reproduction in Domestic Animals | Year: 2014

Contents: Follicular wave emergence was synchronized by treating camels with GnRH when a dominant follicle (DF) was present in the ovaries. Animals were scanned twice a day from day 0 (day of GnRH treatment) to day 10, to characterize emergence and deviation of follicles during the development of the follicular wave. Follicle deviation in individual animals was determined by graphical method. Single DFs were found in 16, double DFs in 9 and triple DFs in two camels. The incidence of codominant (double and triple DFs) follicles was 41%. The interval from GnRH treatment to wave emergence, wave emergence to deviation, diameter and growth rate of F1 follicle before or after deviation did not differ between the animals with single and double DFs. The size difference between future DF(s) and the largest subordinate follicle (SF) was apparent from the day of wave emergence in single and double DFs. Overall, interval from GnRH treatment to wave emergence and wave emergence to the beginning of follicle deviation was 70.6 ± 1.4 and 58.6 ± 2.7 h, respectively. Mean size of the DF and largest SF at the beginning of deviation was 7.4 ± 0.2 and 6.3 ± 0.1 mm, respectively. In conclusion, the characteristics of follicle deviation are similar between the animals that developed single or double DFs. © 2013 Blackwell Verlag GmbH.


PubMed | Laboratories and Animal Research Center
Type: Journal Article | Journal: Reproductive biology | Year: 2015

The acquisition of ovulatory capacity in the growing dominant follicle (DF) of dromedary camels was examined in the current study. Ovulation occurred in response to hCG (1500 IU) in 27.3%, 58.3% or 100% of camels with follicles of 9, 10 or 11 mm diameter, respectively. A high dose of hCG (4500 IU) resulted in ovulation of 77.8% and 100% of camels with follicles of 9 and 10mm, respectively. In naturally mated animals, ovulation occurred in 36.4% and 92.8% of camels with 10 and 11 mm follicles, respectively.


PubMed | Laboratories and Animal Research Center
Type: Journal Article | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2014

Follicular wave emergence was synchronized by treating camels with GnRH when a dominant follicle (DF) was present in the ovaries. Animals were scanned twice a day from day 0 (day of GnRH treatment) to day 10, to characterize emergence and deviation of follicles during the development of the follicular wave. Follicle deviation in individual animals was determined by graphical method. Single DFs were found in 16, double DFs in 9 and triple DFs in two camels. The incidence of codominant (double and triple DFs) follicles was 41%. The interval from GnRH treatment to wave emergence, wave emergence to deviation, diameter and growth rate of F1 follicle before or after deviation did not differ between the animals with single and double DFs. The size difference between future DF(s) and the largest subordinate follicle (SF) was apparent from the day of wave emergence in single and double DFs. Overall, interval from GnRH treatment to wave emergence and wave emergence to the beginning of follicle deviation was 70.6 1.4 and 58.6 2.7 h, respectively. Mean size of the DF and largest SF at the beginning of deviation was 7.4 0.2 and 6.3 0.1 mm, respectively. In conclusion, the characteristics of follicle deviation are similar between the animals that developed single or double DFs.


PubMed | Laboratories and Animal Research Center
Type: Clinical Trial | Journal: Animal reproduction science | Year: 2012

The present study was carried out to elucidate the effect of progesterone (P4) from the induced corpus luteum (CL) on the characteristics of the dominant follicle (DF) in dromedary camels (Camelus dromedarius). Ovarian follicular and induced CL dynamics were monitored by transrectal ultrasonography in eight camels during the peak breeding season. The characteristics of the DF were monitored daily from the day of emergence into a wave, until it appeared to lose its dominance and the DF of a subsequent wave grew to a diameter of 13-17 mm. At this stage ovulation was induced by hCG and the DF was monitored every 8 h for 48 h. After ovulation, CL dynamics and follicular development (emergence of a new wave, growth and mature phase of the selected DF) were monitored daily. Blood samples were collected during each ultrasound examination to study the P4 profile in these animals. The CL developed to a maximum size (22.55 3.24 mm) with a peak concentration of P4 (4.60 2.57 ng/ml) 7 days after ovulation. The size of the CL was positively correlated with the P4 concentration (r = 0.612) during the different stages of the CL dynamics. The presence of CL did not affect the linear growth rate, duration of growth and mature phases of the DF. The development of the DF to its maximum size during its mature phase and inter-wave interval were not affected by the P4 secreted by the induced CL. In conclusion, there is no evidence from this study to suggest that P4 from induced CL altered the characteristics of a DF in dromedary camels.


PubMed | Laboratories and Animal Research Center
Type: Journal Article | Journal: Theriogenology | Year: 2012

Ovarian follicular dynamics was monitored by transrectal ultrasonography, for a period of 60 to 90 days, and its correlation with plasma estradiol-17 (E2) and progesterone (P4) were studied in seventeen, multiparous, non-lactating, 12 to 20-year-old dromedary camels. The average number of follicles recruited (12.77 0.93) in each wave between animals varied (P < 0.001). The number of follicles recruited during different follicular waves was highly repeatable (0.95) within individual animals. The growth and mature phase periods of the dominant follicle (DF) were 6.10 0.15 and 10.20 0.47 days, respectively with a linear growth rate of 1.17 0.02 mm/day between Day 0 and 10 of the follicular wave. There was an inverse relationship between the diameter of the largest DF and number of follicles (r = -0.95, P < 0.001). The DF development did not regularly alternate between the ovaries and the incidence of codominance was 45%. The mean maximum diameter of DF during its mature phase was 27.30 0.78 mm and oversized follicle was 38.43 1.41 mm. In 73.3% waves, the DF continued its growth for a period of 10.64 1.53 days even after losing its dominance and developed into oversized follicle. The duration of the regression phase of DF and oversized follicle were 24.71 3.79 and 18.50 2.23 days. The mean duration of a complete follicular wave was 47.11 2.94 days with an interwave interval (IWI) of 16.36 0.37 days. The IWI within an individual was repeatable (0.88) and between the animals was variable (P < 0.001). Plasma E2 concentration profiles showed a wave like pattern. The peak plasma E2 concentrations were attained approximately 12 days after beginning of the growth phase, when the largest DF grew to a diameter of 18.7 mm. Plasma concentration of P4 was below 1.0 ng/mL in 85% of waves and above 1.0 ng/mL in 15% of the waves for a period of 3 to 6 days in the absence of spontaneous ovulation. It is concluded that ovarian follicular development and plasma E2 concentrations occurs in a wave like pattern in dromedary camels and the IWI and follicle numbers recruited per wave are variable between the animals and repeatable within an individual animal.

Loading Laboratories and Animal Research Center collaborators
Loading Laboratories and Animal Research Center collaborators