Espasa M.,Laboratori Of Microbiologia |
Espasa M.,Autonomous University of Barcelona |
Salvado M.,Autonomous University of Barcelona |
Salvado M.,Laboratori Of Microbiologia |
And 15 more authors.
Journal of Clinical Microbiology | Year: 2012
The aim of this study was to evaluate the reliability of the VersaTREK system for Mycobacterium tuberculosis drug susceptibility testing compared with results obtained with the Bactec MGIT 960 system. A total of 67 strains were evaluated. Overall agreement was at 98.5%. Kappa indexes were 1.0 for isoniazid, rifampin, and ethambutol, 0.937 for pyrazinamide, and 0.907 for streptomycin. The VersaTREK system is validated for M. tuberculosis drug susceptibility testing. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Calvet X.,University of Barcelona |
Calvet X.,CIBER ISCIII |
Lario S.,University of Barcelona |
Lario S.,CIBER ISCIII |
And 21 more authors.
Clinical Infectious Diseases | Year: 2010
Background. Well-devised studies comparing new but different monoclonal fecal tests for diagnosing Helicobacter pylori infection are scarce. The objective of this study was to compare the diagnostic accuracy of 3 monoclonal stool tests: 2 rapid in-office tools - RAPID Hp StAR and ImmunoCard STAT! HpSA - and an enzyme immunoassay test - Amplified IDEIA Hp StAR - for diagnosing H. pylori infection prior to eradication treatment. Methods. Diagnostic reliability was evaluated in 199 untreated consecutive patients with dyspeptic symptoms. The gold standard for diagnosing H. pylori infection was defined as the concordance of the rapid urease test, histopathology, and urea breath test. Readings of immunochromatographic tests were performed by 2 different observers. Sensitivity, specificity, positive and negative predictive values, and 95% confidence intervals were calculated. Sensitivity and specificity were compared using the McNemar test. Results. The sensitivity and specificity of Amplified IDEIA Hp StAR were 90% and 89%, respectively. This enzyme immunoassay test was significantly more sensitive than ImmunoCard STAT! HpSA and more specific than RAPID Hp StAR. The sensitivity and specificity of RAPID Hp StAR were 91% and 80%, respectively, according to observer 1, and 92% and 76%, respectively, according to observer 2. It was significantly more sensitive and less specific than ImmunoCard STAT! HpSA. The sensitivity and specificity of ImmunoCard STAT! HpSA were 69% and 90%, respectively, according to observer 1, and 74% and 89%, respectively, according to observer 2. Conclusions. Amplified IDEIA Hp StAR seems to be the most accurate stool test for diagnosing H. pylori for patients with dyspeptic symptoms. The currently available in-office tests obtain slightly less reliable results. © 2010 by the Infectious Diseases Society of Amenca. All rights reserved.
Calciati E.,Servei dEpidemiologia |
Lafuente S.,Servei dEpidemiologia |
De Simo M.,Laboratori Of Microbiologia |
Balfagon P.,Institute Of Seguretat Alimentaria |
And 2 more authors.
Enfermedades Infecciosas y Microbiologia Clinica | Year: 2012
Introduction: Campylobacter outbreaks are less common and described than sporadic Campylobacteriosis. Methods: We describe the epidemiological investigation including stool examination and bacteriological typing of a Campylobacter outbreak affecting 75 primary school children. Results: The highest risk ratio was associated with the food served 4 days before the peak of cases, namely roast chicken and Russian salad. Discussion: Poor food preparation practices and deficient kitchen facilities appear to be key issues for cross-contamination of Campylobacter from raw chicken to cooked food. © 2011 Elsevier España, S.L.
Garreta A.,Laboratori Of Microbiologia |
Garreta A.,Institute Of Biologia Molecular Ibmb |
Val-Moraes S.P.,Laboratori Of Microbiologia |
Garcia-Fernandez Q.,Institute Of Biologia Molecular Ibmb |
And 8 more authors.
FASEB Journal | Year: 2013
Lipoxygenases (LOXs), which are essential in eukaryotes, have no confirmed function in prokaryotes that are devoid of polyunsaturated fatty acids. The structure of a secretable LOX from Pseudomonas aeruginosa (Pa-LOX), the first available from a prokaryote, presents significant differences with respect to eukaryotic LOXs, including a cluster of helices acting as a lid to the active center. The mobility of the lid and the structural variability of the N-terminal region of Pa-LOX was confirmed by comparing 2 crystal forms. The binding pocket contains a phosphatidylethanolamine phospholipid with branches of 18 (sn-1) and 14/16 (sn-2) carbon atoms in length. Carbon atoms from the sn-1 chain approach the catalytic iron in a manner that sheds light on how the enzymatic reaction might proceed. The findings in these studies suggest that Pa-LOX has the capacity to extract and modify unsaturated phospholipids from eukaryotic membranes, allowing this LOX to play a role in the interaction of P. Aeruginosa with host cells.Garreta, A., Val-Moraes, S. P., García-Fernández, Q., Montserrat Busquets, C. J., Oliver, A., Ortiz, A., Gaffney, B. J., Fita, I., Manresa, A., Carpena, X. Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa. © FASEB.