Laboratori Of Genetica E Microbiologia A Buzzati Traverso

Pavia, Italy

Laboratori Of Genetica E Microbiologia A Buzzati Traverso

Pavia, Italy
SEARCH FILTERS
Time filter
Source Type

Dona M.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Macovei A.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Macovei A.,International Center for Biotechnology and Genetic Engineering | Carbonera D.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Balestrazzi A.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso
Plant Physiology and Biochemistry | Year: 2012

Farmers and growers are constantly looking for high quality seeds able to ensure uniform field establishment and increased production. Seed priming is used to induce pre-germinative metabolism and then enhance germination efficiency and crop yields. It has been hypothesized that priming treatments might also improve stress tolerance in germinating seeds, leaving a sort of 'stress memory'. However, the molecular bases of priming still need to be clarified and the identification of molecular indicators of seed vigor is nowadays a relevant goal for the basic and applied research in seed biology. It is generally acknowledged that enhanced seed vigor and successful priming depend on DNA repair mechanisms, activated during imbibition. The complexity of the networks of DNA damage control/repair functions has been only partially elucidated in plants and the specific literature that address seeds remains scanty. The DNA repair pathways hereby described (Nucleotide and Base Excision Repair, Non-Homologous End Joining, Homologous Recombination) play specific roles, all of them being critical to ensure genome stability. This review also focuses on some novel regulatory mechanisms of DNA repair (chromatin remodeling and small RNAs) while the possible use of telomere sequences as markers of aging in seed banks is discussed. The significant contribution provided by Electron Paramagnetic Resonance in elucidating the kinetics of seed aging, in terms of free radical profiles and membrane integrity is reported. © 2012 Elsevier Masson SAS.


Guzzon F.,Royal Botanic Gardens Kew | Muller J.V.,Royal Botanic Gardens Kew | Cauzzi P.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Balestrazzi A.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso
Acta Botanica Gallica | Year: 2015

The genus Aegilops represents the secondary gene pool of wheat. A better understanding of the germination behaviour of Aegilops species is important to improve their use in breeding programmes and strengthen their in situ/ex situ conservation. In this study, we investigated the germination behaviour of nine Aegilops species, five of them not yet investigated, under two alternating and two constant temperatures. Seed germination was higher than 60% in all temperature treatments and species. Alternating temperatures promoted higher germination rates in all the species and significantly enhanced seed germination in three species (Aegilops biuncialis, Aegilops geniculata and Aegilops neglecta), in comparison with constant temperatures. One of the rarest and least productive species in the genus (Aegilops uniaristata) displayed the highest germination. This study suggests that different (seed) regeneration strategies/niches occur across Aegilops species. © 2015 Société botanique de France.


Giovannini A.,Italian Agricultural Research Council | Dona M.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Macovei A.,Laboratori Of Genetica E Microbiologia A Buzzati Traverso | Macovei A.,International Center for Biote chnology and Genet icEngineering | And 2 more authors.
Chemosphere | Year: 2013

Single Cell Gel Electrophoresis is currently used to investigate the cell response to genotoxic agents as well as to several biotic and abiotic stresses that lead to oxidative DNA damage. Different versions of Single Cell Gel Electrophoresis have been developed in order to expand the range of DNA lesions that can be detected and guidelines for their use in genetic toxicology have been provided. Applications of Single Cell Gel Electrophoresis in plants are still limited, compared to animal systems. This technique is now emerging as a useful tool in assessing the potential of higher plants as stable sensors in ecosystems and source of information on the genotoxic impact of dangerous pollutants. Another interesting application of Single Cell Gel Electrophoresis deals with Mutation Breeding or the combined use of irradiation and in vitro culture technique to enhance genetic variability in elite plant genotypes. SCGE, in combination with in situ detection of Reactive Oxygen Species (ROS) induced by γ-rays and expression analysis of both DNA repair and antioxidant genes, can be used to gather information on the radiosensitivity level of the target plant genotypes. © 2013 Elsevier Ltd.

Loading Laboratori Of Genetica E Microbiologia A Buzzati Traverso collaborators
Loading Laboratori Of Genetica E Microbiologia A Buzzati Traverso collaborators