Berger M.,C.E.A. |
Cailler F.,Montpellier University |
Chambron J.,Bugatti |
Coll J.L.,French Institute of Health and Medical Research |
And 7 more authors.
IRBM | Year: 2010
Objective: The meaning of this work was to develop a new medical imaging technique aimed to assist the surgical act during the resection of cancerous tumors. Method: This technique is based on both the specificity of anti-tumor monoclonal antibodies or anti-neovessels peptides and the sensitivity of fluorescent detection. The objective was to develop and offer to surgeons the most effective vector-fluorophore-detection probe association in order to help them to significantly improve the patient's recovery. The development of a fluorescent detection probe fulfils two requirements: avoiding the injection of radioactive molecules which calls for difficult protection procedures and offering such detection to abdominal surgery, for which the background noise caused by the use of radioisotopes makes this technique hardly efficient. The experimental part of the project had the aim of comparing, within animals, the sensitivity and specificity of these techniques. Preclinical study has also been realized for fluorescent markers. Such studies had to show the benefits of the optical method compared to the radioisotopic method for oncology surgeons. Results: An industrial prototype of the probe has been developed and tested on animals after injection of the tumor specific molecule conjugated to a fluorescent dye developed within the frame of the project. The digestive tumors could perfectly be visualised. Conclusion: This surgical imaging technique should have a major impact on the practices of oncology surgeons and should lead to less complementary therapies and therefore more comfort for the patients. © 2010 Elsevier Masson SAS. All rights reserved.
Laboratoires Synth Innove | Date: 2011-09-09
Labels are disclosed capable of forming a covalent or non-covalent bond with a target molecule, particularly a biological molecule. The structure of these labels may consist of a dye covalently bound by one or more carbons on its chemical structure to one or more [FUNC] group(s), and optionally one or more [SOL] group(s). The structure of these labels allow selection of dyes from a wide variety of different excitation and emission wavelengths and allow easy functionalization of the dye without appreciably altering its spectral characteristics or its solubility characteristics.