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Saint-Malo, France

Gauthier A.,CNRS Agroecology Lab | Trouvelot S.,CNRS Agroecology Lab | Kelloniemi J.,CNRS Agroecology Lab | Frettinger P.,CNRS Agroecology Lab | And 7 more authors.
PLoS ONE | Year: 2014

Grapevine (Vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. Chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. The β-glucan laminarin (Lam) and its sulfated derivative (PS3) have been previously demonstrated to induce resistance in grapevine against downy mildew (Plasmopara viticola). However, if Lam elicits classical grapevine defenses such as oxidative burst, pathogenesis-related (PR)-proteins and phytoalexin production, PS3 triggered grapevine resistance via a poorly understood priming phenomenon. The aim of this study was to identify the molecular mechanisms of the PS3-induced resistance. For this purpose we studied i) the signaling events and transcriptome reprogramming triggered by PS3 treatment on uninfected grapevine, ii) grapevine immune responses primed by PS3 during P. viticola infection. Our results showed that i) PS3 was unable to elicit reactive oxygen species (ROS) production, cytosolic Ca2+ concentration variations, mitogen-activated protein kinase (MAPK) activation but triggered a long lasting plasma membrane depolarization in grapevine cells, ii) PS3 and Lam shared a common stress-responsive transcriptome profile that partly overlapped the salicylate- (SA) and jasmonate-(JA)-dependent ones. After P. viticola inoculation, PS3 specifically primed the SA- and ROS-dependent defense pathways leading to grapevine induced resistance against this biotroph. Interestingly pharmacological approaches suggested that the plasma membrane depolarization and the downstream ROS production are key events of the PS3-induced resistance. © 2014 Gauthier et al. Source


Steimetz E.,CNRS Agroecology Lab | Trouvelot S.,CNRS Agroecology Lab | Gindro K.,Agroscope Changins Wadenswil | Bordier A.,Laboratoires Goemar | And 3 more authors.
Physiological and Molecular Plant Pathology | Year: 2012

Sulfated laminarin (PS3) has previously been shown to induce resistance of grapevine leaves against the oomycete Plasmopara viticola, the causal agent of grape downy mildew. Here, we observed that the level of PS3-induced resistance (PS3-IR) was higher in the adult leaf (in position P3) than in the younger, not fully expanded leaf (in position P1, located above P3). By investigating grapevine defense reactions upon PS3 treatment and inoculation, we found that the production of H2O2, of phytoalexins, and the deposition of phenolics were more abundant in P3 than in P1 leaves. In addition, PS3 significantly reduced stomatal colonization by zoospores only in P3 leaves. Thus, the capacity of an adult leaf to express a higher level of defense reactions during PS3-IR may partly explain why it exhibits a more elevated resistance when compared to a young leaf, still in growth. These findings have likely practical consequences in induced resistance application. © 2012 Elsevier Ltd. Source


Trademark
Laboratoires Goemar | Date: 2011-08-23

Chemical preparations intended for agriculture, horticulture and forestry excluding fungicides, herbicides, insecticides and parasite killers; fertilizers; fertilizers in the nature of seaweed-based fertilizer products; soil amendments, namely, biostimulants, physiological activators in the nature of plant growth activators and fertilizers for plant nutrition; seaweed-based chemical activators for the nutrition and growth regulation of plants, trees, vines, cereals, fruit and vegetables; fertilizers for growing seaweed.


Patent
Laboratoires Goemar | Date: 2011-07-01

The invention relates to the use of an extract of grape marc for promoting plant growth and to a method for promoting plant growth which comprises the application of a composition comprising an extract of grape marc to said plants.


The present invention relates to solutions including at least one boron complex obtained through the reaction between at least one boron salt, consisting of a borate anion selected from within the group consisting of metaborate anions, tetraborate anions, pentaborate anions, octaborate anions, decaborate anions, and the mixtures thereof, and a cation selected from within the group including sodium cations, potassium cations, ammonium cations, and the mixtures thereof, and at least one polyol and at least one amino compound. The basic boron concentration is greater than 2 wt % of the total weight of the solution. The invention also relates to a method for preparing said solutions and to the uses of said solutions, particularly in the field of agriculture.

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