Cuzick J.,Queen Mary, University of London |
Bergeron C.,Laboratoire Pasteur Cerba |
von Knebel Doeberitz M.,University of Heidelberg |
Jeronimo J.,PATH |
And 5 more authors.
Vaccine | Year: 2012
The clearly higher sensitivity and reproducibility of human papillomavirus (HPV) DNA testing for highgrade cervical intraepithelial neoplasia (CIN) has led to widespread calls to introduce it as the primary screening test. The main concern has been its lower specificity, due to the fact that it cannot separate transient from persistent infections, and only the latter are associated with an increased risk of high-grade CIN and cancer. Thus, even proponents of HPV testing generally only recommend it for women over the age of 30 years (or in some cases 35 years). If HPV testing is to reach its full potential, new approaches with better specificity are needed, either as triage tests for HPV positive women or, if the high sensitivity of HPV DNA testing can be maintained, as alternate primary screening modalities. Approaches that may useful in this regard, especially as triage tests, include HPV typing, methylation (and consequent silencing) of host and viral genes, and new cytologic methods, such as p16INK4a staining, which attempt to identify proliferating cells. At an earlier stage of development are direct methods based on detection of HPV E6 or E7 proteins. Recent progress and current status of these methods is discussed in this chapter. The current status of visual inspection (VIA and VILI) methods is also surveyed and progress on self-sampling is reviewed. © 2012 Published by Elsevier Ltd.
PubMed | The Doctors Laboratory, Royal Free Hospital, Laboratoire Pasteur Cerba, University of Virginia and 3 more.
Type: Comparative Study | Journal: European journal of cancer (Oxford, England : 1990) | Year: 2014
To compare triage strategies using different human papillomavirus (HPV) consensus and genotyping tests and a p16(INK4a) test.1228 women referred with a borderline or single mildly dyskaryotic smear. Samples were taken at colposcopy using PreservCyt. Tests included Hybrid Capture 2, Abbott RealTime PCR, BD HPV, Cobas 4800, PreTect HPV-Proofer, APTIMA and p16(INK4a). Results were based on the worst histology within 9 months.97/1228 (7.9%) women had CIN3+ (203/1228 (17%) CIN2+). HPV testing alone using Hybrid Capture 2, Abbott RealTime PCR, BD HPV, Cobas 4800 or APTIMA had a sensitivity for CIN3+ ranging from 99.0% to 100.0% and specificity for
Tarte K.,University of Rennes 1 |
Tarte K.,Rennes University Hospital Center |
Gaillard J.,Center Atlantique |
Gaillard J.,University of Tours |
And 14 more authors.
Blood | Year: 2010
Clinical-grade human mesenchymal stromal cells (MSCs) have been expanded in vitro for tissue engineering or immunoregulatory purposes without standardized culture conditions or release criteria. Although human MSCs show poor susceptibility for oncogenic transformation, 2 recent studies described their capacity to accumulate chromosomal instability and to give rise to carcinoma in immunocompromised mice after long-term culture. We thus investigated the immunologic and genetic features of MSCs expanded with fetal calf serum and fibroblast growth factor or with platelet lysate in 4 cell-therapy facilities during 2 multicenter clinical trials. Cultured MSCs showed a moderate expression of human leukocyte antigen-DR without alteration of their low immunogenicity or their immunomodulatory capacity. Moreover, some transient and donor-dependent recurring aneuploidy was detected in vitro, independently of the culture process. However, MSCs with or without chromosomal alterations showed progressive growth arrest and entered senescence without evidence of transformation either in vitro or in vivo. © 2010 by The American Society of Hematology.
Carod J.-F.,Institute Pasteur Of Madagascar |
Randrianarison M.,Center hospitalier Soavinandriana |
Razafimahefa J.,Service de Neurologie |
Ramahefarisoa R.M.,Institute Pasteur Of Madagascar |
And 6 more authors.
Diagnostic Microbiology and Infectious Disease | Year: 2012
This study aimed to evaluate 5 enzyme immunoassays for detecting human antibodies against Taenia solium in human serum and for the diagnosis of neurocysticercosis (NCC): DRG RIDASCREEN, NOVATECH, CYPRESS, and IVD A collection of 114 reference serum samples were used. All sera were tested both by ELISA and by an immunoblot method (enzyme-linked immunoelectrotransfer blot [EITB]). When compared with EITB, the Ridascreentest had the best positive concordance rate (85.1-91.2%) and the NovaLisa test showed the optimal negative concordance rate (93.7-95.6%). All tests had a sensitivity under 72% and a specificity above 60%. The best sensitivity was obtained using Ridascreen test (71.4%). An optimal specificity was achieved by the NovaLisa test. T. solium-positive sera all cross-reacted with E. granulosus positive samples. In the commercial assays evaluated here, the most appropriate ELISA test for screening may be the Ridascreen assay. Antibody detection seems to be not appropriate for NCC diagnosis because of its overall lack of sensitivity. © 2012 Elsevier Inc.
Zanella-Cleon I.,University Claude Bernard Lyon 1 |
Delolme F.,University Claude Bernard Lyon 1 |
Lacan P.,Laboratoire Of Biochimie Et Of Biologie Moleculaire |
Garcia C.,Laboratoire Of Biochimie Et Of Biologie Moleculaire |
And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012
DNA analysis is currently the easiest way to identify a hemoglobin variant in most cases. Nevertheless, in case of complex gene rearrangements, mass spectrometry studies may be required to orientate the DNA diagnosis. The present report shows the use of mass spectrometry techniques prior to DNA analysis for the identification of the rare P-Nilotic fusion hemoglobin. Complete protein analysis is performed by liquid chromatography-tandem mass spectrometry on the abnormal globin chain isolated by reversed-phase liquid chromatography. © 2011 Elsevier B.V..
Szarewski A.,University of London |
Mesher D.,University of London |
Cadman L.,University of London |
Austin J.,University of London |
And 12 more authors.
Journal of Clinical Microbiology | Year: 2012
High-risk human papillomavirus (HPV) DNA/RNA testing provides higher sensitivity but lower specificity than cytology for the identification of high-grade cervical intraepithelial neoplasia (CIN). Several new HPV tests are now available for this purpose, and a direct comparison of their properties is needed. Seven tests were evaluated with samples in liquid PreservCyt transport medium from 1,099 women referred for colposcopy: the Hybrid Capture 2 (Qiagen), Cobas (Roche), PreTect HPV-Proofer (NorChip), Aptima HPV (Gen-Probe), and Abbott RealTime assays, the BD HPV test, and CINtec p16INK4a cytology (mtm laboratories) immunocytochemistry. Sensitivity, specificity, and positive predictive value (PPV) were based on the worst histology found on either the biopsy or the treatment specimen after central review. Three hundred fifty-nine women (32.7%) had CIN grade 2+(CIN2+), with 224 (20.4%) having CIN3+. For detection of CIN2+, Hybrid Capture 2 had 96.3% sensitivity, 19.5% specificity, and 37.4% PPV. Cobas had 95.2% sensitivity, 24.0% specificity, and 37.6% PPV. The BD HPV test had 95.0% sensitivity, 24.2% specificity, and 37.8% PPV. Abbott RealTime had 93.3% sensitivity, 27.3% specificity, and 38.2% PPV. Aptima had 95.3% sensitivity, 28.8% specificity, and 39.3% PPV. PreTect HPV-Proofer had 74.1% sensitivity, 70.8% specificity, and 55.4% PPV. CINtec p16INK4a cytology had 85.7% sensitivity, 54.7% specificity, and 49.1% PPV. Cytology of a specimen taken at colposcopy (mild dyskaryosis or worse) had 88.9% sensitivity, 58.1% specificity, and 50.7% PPV. Our study confirms that, in a referral setting, HPV testing by a number of different tests provides high sensitivity for high-grade disease. Further work is needed to confirm these findings in a routine screening setting. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Lesesve J.-F.,Nancy University Hospital Center |
Gressot A.-L.,Nancy University Hospital Center |
Troussard X.,Caen University Hospital Center |
Mossafa H.,Laboratoire Pasteur Cerba |
Cornet E.,Caen University Hospital Center
Leukemia and Lymphoma | Year: 2014
The observation of binucleated lymphocytes (BNLs) on a peripheral blood smear is essential to the diagnosis of persistent polyclonal B-cell lymphocytosis (PPBL). Only a few case reports have mentioned their presence in other contexts, mainly mature B-cell neoplasms such as chronic lymphocytic leukemia or reactive circumstances such as multiple sclerosis undergoing natalizumab treatment. We sought to investigate whether any particular morphologic features of BNL were more specific to PPBL than other diseases. We reviewed peripheral blood smears of a series of patients affected by PPBL or harboring BNLs whatever the diagnosis. We found that BNLs in PPBL were heterogeneous, but mostly medium-sized with a moderately abundant basophilic cytoplasm, sometimes vacuolated. The chromatin was mature in an asymmetrically bilobed nucleus showing one to two nucleoli. Though mainly observed in patients with PPBL, all these criteria remained non-specific. Conversely, the presence of either monocytoid or hyperbasophilic cells had clinical value to confirm PPBL. We conclude that a substantial percentage of BNLs possessing the morphologic features described and observed in a context of monocytoid and/or hyperbasophilic lymphocytes is predictive of the diagnosis of PPBL. © 2014 Informa UK, Ltd.
Cavalier E.,University of Liège |
Rozet E.,University of Liège |
Gadisseur R.,University of Liège |
Carlisi A.,University of Liège |
And 5 more authors.
Osteoporosis International | Year: 2010
Due to "measurement uncertainty", the "true" 25-OH vitamin D (25(OH)D) of a patient (whatever the commercially available assay tested) will be >80 nmol/L if its measured concentration is >100 nmol/L. Thus, if a physician considers that a normal VTD status is a 25(OH)D level ≥80 nmol/L, he should ensure that the patient's results are ≥100 nmol/L. Introduction: Many experts recommend that serum levels of 25(OH)D should be above a lower normal limit of 75-C 80 nmol/L. However, the value delivered by laboratories is only an estimation of the "true" value due to "measurement uncertainty." When using a cut off, measurement uncertainty around the cut off is important because therapeutic actions may differ if the measured value is below or above the limit. We aimed to establish the "measurement uncertainty" at different levels of concentration for several commercially available 25(OH)D analytical techniques. Methods: We constituted three pools of serum with different 25(OH)D concentrations. Each pool was assayed in triplicate during 5 days with the DiaSorin RIA, Liaison, Elecsys, and Chromsystems-HPLC assays. Results: We report a relatively high "measurement uncertainty" for the measurement of 25(OH)D for the four different techniques: the mean relative uncertainties, all techniques confounded were 19.4%, 16.0%, and 11.3% for pool 1 (35.3 nmol/L), pool 2 (79.5 nmol/L), and pool 3 (126.1 nmol/L), respectively. Conclusions: Our results show that, whatever the assay, the "true" 25(OH)D of a patient will be >80 nmol/L if its measured concentration is >100 nmol/L. In other words, if a physician considers that a normal VTD status is defined by a 25(OH)D level ≥80 nmol/L, he should ensure that the patients present a 25(OH)D ≥100 nmol/L. © International Osteoporosis Foundation and National Osteoporosis Foundation 2009.
Cuzick J.,Queen Mary, University of London |
Ambroisine L.,Queen Mary, University of London |
Cadman L.,Queen Mary, University of London |
Austin J.,Queen Mary, University of London |
And 10 more authors.
Journal of Medical Virology | Year: 2010
HPV DNA testing is known to be much more sensitive than cytology, but less specific. A range of HPV and related tests in 858 women referred for colposcopy because of an abnormal smear were evaluated to compare the performances of these tests. This article compared the Abbott test to other tests which had been previously evaluated. This test was a real true test for 14 high-risk HPV types. The Abbott test was found to be highly sensitive for cervical intraepithelial neoplasia grade 3 or worse (CIN3+) (98.9%) with a specificity of 31.5%. These numbers were comparable with the Qiagen HC2 test, the Roche Linear Array and Amplicor tests, and the Gen-Probe APTIMA test. Differences between these tests appeared to be related mostly to the choice of cutoff level. An added feature of the Abbott test was the provision of type specific results for HPV 16 and 18. © 2010 Wiley-Liss, Inc.
Trombert-Paolantoni S.,Laboratoire Pasteur Cerba |
Thomas P.,Laboratoire Pasteur Cerba |
Hermet F.,Laboratoire Pasteur Cerba |
Clairet V.,Laboratoire Pasteur Cerba |
And 2 more authors.
Pathologie Biologie | Year: 2010
Aim of the study: Evaluate the sensitivity and the specificity of anti- Leptospira IgM detection with the Serion Elisa classic kit in comparison to the Panbio Elisa kit. Methods: Twenty-three sera of patients whom Taqman probe real time PCR is positive and 19 sera positive by the microscopic agglutination test (MAT) are studied for sensitivity. Specificity is evaluated with 49 pairs of sera negative by MAT and with sera positive in IgM to EBV, to syphilis, to Borrelia and positive to influenza virus antibodies. Results: 14/23 (61%) of the sera positive by PCR and 27/30 (90%) of the sera positive at significant level by MAT are positive by Serion IgM Elisa. Serogroups Icterohaemorrhagiae, Grippotyphosa, Australis, Tarassovi, Canicola, Sejroe, Patoc and Cynopteri are detected. 40/49 sera (82%) negative by MAT are negative by Serion IgM Elisa. Agreement with the Panbio kit is 61% (30/49). Results are discrepant in 19/49 cases corresponding to a positive result by Panbio IgM and to a negative result by Serion IgM. Serological cross-reactions are more frequent with IgM to syphilis and to Borrelia than with IgM to EBV and to influenza virus antibodies. Conclusion: A similar sensitivity is observed between the two kits but specificity is higher with Serion. Positivity in IgM is 61% in cases of leptospirosis diagnosed by PCR. © 2009.