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Port-Saint-Louis-du-Rhône, France

Bourin P.,Laboratoire Of Therapie Cellulaire
Methods in molecular biology (Clifton, N.J.) | Year: 2011

Adipose tissue-derived stromal cells (ASCs) are promising tools for the new therapeutic field of regenerative medicine. Many research teams are intent on producing these cells for therapeutic purposes. The cell production must follow strict rules for safety and for constant quality of the cell product to ensure a reliable effect in patients. These rules are grouped under the generic term Good Manufacturing Practices. In this chapter, we describe the general concepts of ASC production for therapeutic use, explaining new terms such as traceability and qualification. We also introduce general requirements for the installation, equipment, material, and staff for the cell production. Then, we outline a general strategy for building a cell culture process. Finally, as an example, we describe the use of CellStack™ chambers and specific tube sets that allow for producing cells beginning with the stromal vascular fraction under near-closed conditions. Source

Boussion H.,Service dOncologie Medicale | Chaput N.,French Institute of Health and Medical Research | Chaput N.,Laboratoire Of Therapie Cellulaire
Oncologie | Year: 2015

Exosomes are nanovesicles released by a large number of cells in their physiological and pathological states. They have a specific composition and are formed from cytosolic proteins and nucleic acids surrounded by a complex lipid membrane. They play a significant role in intercellular exchanges and the regulation of immune responses. In tumour development, they are involved in angiogenesis, cell proliferation, the spread of metastases and in the regulation of cells responsible for anti-tumour immunity. They can be found within tumour cells and in the microenvironment cells, where they enable the metastatic niche to be created. In clinical practice with bronchial carcinoma, the use of exosomes has been developed not only as a diagnostic tool with “fluid biopsies” but also as a means of treatment, in particular reactivating the anti-tumour immune system. © 2015, Springer-Verlag France. Source

Ceccaldi C.,CNRS Inter-university Material Research and Engineering | Ceccaldi C.,French Institute of Health and Medical Research | Bushkalova R.,CNRS Inter-university Material Research and Engineering | Bushkalova R.,French Institute of Health and Medical Research | And 16 more authors.
Acta Biomaterialia | Year: 2014

Three-dimensional (3D) scaffolds hold great potential for stem cell-based therapies. Indeed, recent results have shown that biomimetic scaffolds may enhance cell survival and promote an increase in the concentration of therapeutic cells at the injury site. The aim of this work was to engineer an original polymeric scaffold based on the respective beneficial effects of alginate and chitosan. Formulations were made from various alginate/chitosan ratios to form opposite-charge polyelectrolyte complexes (PECs). After freeze-drying, the resultant matrices presented a highly interconnected porous microstructure and mechanical properties suitable for cell culture. In vitro evaluation demonstrated their compatibility with mesenchymal stell cell (MSC) proliferation and their ability to maintain paracrine activity. Finally, the in vivo performance of seeded 3D PEC scaffolds with a polymeric ratio of 40/60 was evaluated after an acute myocardial infarction provoked in a rat model. Evaluation of cardiac function showed a significant increase in the ejection fraction, improved neovascularization, attenuated fibrosis as well as less left ventricular dilatation as compared to an animal control group. These results provide evidence that 3D PEC scaffolds prepared from alginate and chitosan offer an efficient environment for 3D culturing of MSCs and represent an innovative solution for tissue engineering. © 2013 Acta Materialia Inc. Source

Casteilla L.,Toulouse 1 University Capitole | Casteilla L.,French National Center for Scientific Research | Planat-Benard V.,Toulouse 1 University Capitole | Planat-Benard V.,French National Center for Scientific Research | And 6 more authors.
Current Stem Cell Research and Therapy | Year: 2010

Adipose tissue is the final tissue to develop and is strongly involved in energy homeostasis. It can represent up to 50% of body weight in obesity. Beside its metabolic role, endocrine functions appeared to play a key role in interconnecting adipose tissue with other tissues of the organism and in numerous physiological functions. The presence of adipocyte progenitors has long been demonstrated throughout life in the stromal fraction of adipose tissue. Now, it appears that these cells are multipotent and share numerous features with mesenchymal stem cells (MSC) derived from bone marrow. They also display some specificities and a strong pro-angiogenic potential. Altogether, these data emphasize the need to reconsider the potential of adipose tissue. Moreover, since fat pads are easy to sample, numerous and promising perspectives are now opening up in regenerative medicine, particularly in ischemic situations. © 2010 Bentham Science Publishers Ltd. Source

Chaput N.,French Institute of Health and Medical Research | Chaput N.,Institute Gustave Roussy | Chaput N.,Center dInvestigation Clinique en Biotherapie 507 | Chaput N.,Laboratoire Of Therapie Cellulaire | And 3 more authors.
Seminars in Immunopathology | Year: 2010

To communicate, cells are known to release in their environment proteins which bind to receptors on surrounding cells. But cells also secrete more complex structures, called membrane vesicles, composed of a lipid bilayer with inserted transmembrane proteins, enclosing an internal content of hydrophilic components. Exosomes represent a specific subclass of such secreted membrane vesicles, which, despite having been described more than 20 years ago by two groups studying reticulocyte maturation, have only recently received attention from the scientific community. This renewed interest originated first from the description of exosome secretion by antigen-presenting cells, suggesting a potential role in immune responses, and very recently by the identification of the presence of RNA (both messenger and microRNA) in exosomes, suggesting a potential transfer of genetic information between cells. In this review, we will describe the conclusions of 20 years of studies on the immune properties of exosomes and the most recent advances on their roles and potential uses as markers or as therapeutic tools during pathologies, especially in cancer. © 2010 Springer-Verlag. Source

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