Laboratoire Of Physiologie Animale

Cameroon

Laboratoire Of Physiologie Animale

Cameroon
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Hien O.C.,UCAO UUB | Diarra B.,UPB IDR | Brillard J.-P.,French National Institute for Agricultural Research | Boly H.,UPB IDR | Sawadogo L.,Laboratoire Of Physiologie Animale
International Journal of Poultry Science | Year: 2011

This study concerns the effect of improving health status on testicular development of local guinea fowl reared under natural photoperiod. Two groups of 100 males of guinea fowls chicks were used for the study. One group (T1) served as control and did not received any treatment while the second group (T2) received several treatments including anti-stress, trichonomacid, anticoccidies, dewormer and vaccination against New Castle disease. Results showed no difference in body weight at 28 weeks of age between the 2 groups. However at 24 weeks of age birds in T2 had higher values for mean testicle weight (286±70 versus 148±34 mg). Parameters for seminiferous tubes were also higher for T2 birds: mean volume (74±0.3 versus 63±6.6% of testicle volume), mean diameter (126±24 versus 89±13 μ) and mean length (6.4±0.4 versus 5.1±1 mm). Improved health status also increased population numbers of spermatocytes I, rounds spermatides and interstitial cells. Differences were significant at 20 weeks of age for Sertoli cells. Spermatogenesis starts at 24 weeks of age for T2 birds but at 28 weeks for T1 birds. © Asian Network for Scientific Information, 2011.


PubMed | EA 4267 Fonctions et Dysfonctions Epitheliales, Laboratoire Of Physiologie Animale and Université Ibn Tofail
Type: Journal Article | Journal: Journal of ethnopharmacology | Year: 2013

The stem bark of Terminalia superba (TS) is widely used as a decoction by Cameroonian folk medicine for the treatment of hypertension. The aim of the present study was to evaluate the effect of a chronic treatment with a TS extract on spontaneously hypertensive rats (SHR) with respect to efficacy, biochemical mechanisms and safety.Eleven-week-old SHR and normotensive Wistar Kyoto rats (WKY) were daily treated by gavage with a methylene chloride extract of stem bark of Terminalia superba (TMSE, 150mg/kg) or with the vehicle for 5 weeks. Systolic blood pressure (SBP) was measured weekly using the tail-cuff method. At the end of the treatment period, vascular function was assessed on isolated thoracic rings, urinary 8-iso-PGF2 levels were measured and cytochrome P-450 3A (CYP 3A) activity was evaluated in liver microsomes.TMSE reduced SBP (P<0.001) in SHR but not in WKY rats. In SHR, the vasorelaxant response to acetylcholine was significantly improved by TMSE as a result of increased nitric oxide synthase (NO) activity and decreased superoxide anion production. In addition, TMSE reduced the vasoconstrictive effect of phenylephrine and improved the sensitivity of smooth muscle cells to NO. TMSE dramatically decreased 8-iso-PGF2 levels in SHR. By contrast, TMSE did not affect all these parameters in WKY rats. Neither diuresis nor the hepatic CYP 3A activity was modified in both animal groups.This study demonstrated that Terminalia superba has a potent antihypertensive activity in SHR which is partly due to endothelium-dependent and endothelium-independent effects as well as decreased oxidative stress. The data also provide evidence for the lack of herb-drug interaction through hepatic CYP 3A.


Noumi V.N.,University of Yaounde I | Zapfack L.,University of Yaounde I | Kengne O.C.,University of Yaounde I | Mokoso J.D.M.,University Officielle Of Bukavu | And 5 more authors.
International Journal of Environmental Studies | Year: 2011

The goal was to establish an ecological characterisation of the genus. P. pellucida is a terrestrial species among the African genus of Peperomia. P. molleri is often found terrestrial in the understorey. Most of the species are epiphytes and especially herbaceous. The abundance of the individuals in the low and medium levels of phorophytes shows that life is closely related to humidity. The biotic interactions of the epiphytic species with the mosses have improved their mineral and water uptake. The absence of the members in the hot areas and degraded ecosystems gives reason for the genus to be a bio-indicator. P. pellucida is the only ruderal species while the others are common in sub-mountain and mountain forests. They are abundant between 1000-2500 m. The distribution extends from Senegal to Tanzania with a remarkable abundance in the low Guinean region and Congo basin. Some species are widely distributed; others are restricted to certain phytochoria. © 2011 Taylor & Francis.


Saadi L.,Blida University | Lebaili N.,Laboratoire Of Physiologie Animale
Comptes Rendus - Biologies | Year: 2012

The objective of this work is to evaluate the aldosterone level and to study the gerbil adrenal zona glomerulosa morphology in the presence of an important water diet content during seven days. Thirty adult gerbils, Gerbilus tarabuli, were randomly assigned to two watering regimes, with fifteen gerbils per group. The first group was the control and the second group was exposed to a water-rich diet during seven days. Thin sections of the adrenal glands were observed in photonic and electron microscopy. Their blood was sampled and analyzed for aldosterone, electrolyte concentrations and hematocrit. Urine was collected after 24 hours. A water-rich diet decreased the body weight but increased the hematocrit, total protein, aldosterone concentration and urine volume. In electronic microscopy, the adrenal zona glomerulosa cells present a decrease in lipid compartment and Golgi apparatus zone development. The blood electrolyte concentrations were not changed significantly. These findings indicate that the water-rich diet exerts a potent stimulatory effect on aldosterone secretion by the gerbil adrenal zona glomerulosa through plasma volume perturbation. © 2011 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.


Dridi I.,Laboratoire Of Physiologie Animale | Ben Saad M.,Laboratoire Of Physiologie Animale | Maurel D.,Aix - Marseille University | Bitri L.,Laboratoire Of Physiologie Animale
Biological Rhythm Research | Year: 2013

In this study, we examined some aspects of the chronotoxicity of hexachlorobenzene (HCB) on the liver of rats. Two series of animals received, daily, an oral dose of 16 mg of HCB kg-1 b wt in the beginning or at the end of the lighting phase. The treatment lasted 30 days under a 12L/12D photoperiod. The evolution of some enzymatic and metabolic indicators associated with histomorphological alterations is studied as measures of hepatocellular. The transaminase and Phosphatase alcaline activities appeared to increase significantly at the end of the light phase. Gamma glutamyl transferase (GGT) increased in the AM-treated group comparatively to its witnesses and to the PM groups.The rate of triglycerides presents a significant increase in PM-treated animals. These findings point out the elevated HCB toxicity for rats dosed near the end of their light/rest cycle, probably due to the feeding behaviour of this animal and provide additional support for the dosing-time influence of toxicants. © 2013 Copyright Taylor and Francis Group, LLC.


Sebai H.,University of Carthage | Sebai H.,Institute Superieur Of Biotechnologie Of Beja | Selmi S.,Institute Superieur Of Biotechnologie Of Beja | Selmi S.,Laboratoire Of Physiologie Animale | And 3 more authors.
Journal of Medicinal Food | Year: 2015

The authors aimed in the present study to assess the protective effect of Rosmarinus officinalis essential oils (ROEO) and Lavandula stoechas essential oils (LSEO) against reproductive damage and oxidative stress in alloxan-induced diabetic male rats. Essential oil samples were obtained from the aerial parts of the plants by hydrodistillation and analyzed by the gas chromatography-mass spectrometry (GC-MS). Rats were divided into four groups: healthy control (HC); diabetic control (DC); healthy+ROEO (H+ROEO), healthy+LSEO (H+LSEO), diabetic+ROEO (D+ROEO), and diabetic+LSEO (D+LSEO). The use of GC-MS allowed to the identification of 15 and 22 compounds in ROEO and LSEO, respectively. In addition, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test showed that ROEO and LSEO had an important antioxidant capacity. In vivo, we initially found that ROEO and LSEO treatment protected against the decrease in alloxan-induced body weight gain, relative reproductive organ weights, testosterone level, as well as sperm quality decline. On the other hand, we showed that alloxan administration was accompanied by an oxidative stress status assessed by an increase of malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels, as well as a depletion of sulfhydril group content (-SH) and antioxidant enzyme activities as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in testis, epididymis, and sperm. More importantly, ROEO and LSEO treatment significantly protected against oxidative damage of the male reproductive organ systems in alloxan-induced diabetic rats. These findings suggested that ROEO and LSEO exerted a potential protective effect against alloxan-induced reproductive function damage and oxidative stress in male rat. The beneficial effect of ROEO and LSEO might be related, in part, to their antioxidant properties. © Copyright 2015, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition

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