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Dah-Nouvlessounon D.,University of Benin | Adoukonou-Sagbadja H.,University of Benin | Diarrassouba N.,University Peleforo Gon Coulibaly Of Korhogo Ufr Des Science Biologiques | Sina H.,University of Benin | And 5 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: The aim of this study was to make a phytochemical screening and measure some biological activities of Cola acuminata’s seeds and leaves extracts. Methods: The secondary metabolite was detected by the method based on coloring and precipitation differential reactions. The antibacterial and antifungal activities were assessed in vitro by the macrodillution and solid medium agar diffusion method. 2,2-diphényl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods were used to evaluate the antioxidant activity of the tested extracts of which larval cytotoxicity was studied. Results: Our data revealed that the seeds contain more polyphenols than the leaves. The seeds extracts displayed a good antibacterial activity against both reference strains and food Staphylococcus strains. The inhibition diameters varied from 7±2.82 to 21.5±4.94 mm. The smallest Minimum Inhibitory Concentrations (MIC) on the food strains (0.08 mg/ml) is twice less than the reference strains one (0.15 mg/ml), this observation is contrary to the Minimum Bactericidal concentrations (MBC). Susceptibility of fungal strains varies according to the extracts (p = 0.0016). The seeds ethyl acetate extracts had the best antioxidant activity with DPPH and ABTS methods. The Lethal Doses (LD50) showed that no extract was toxic. Conclusion: Cola acuminata seeds are richer than the leaves in compounds with biological activities. These two organs have interesting antimicrobial, antifungal and antioxidant activity variables depending to the extracts. The seeds have better biological activity than the leaves. © 2015, International Journal of Pharmacy and Pharmaceutical Sciences. All Rights Reserved. Source

Dah-Nouvlessounon D.,University Abomey Calavi | Adoukonou-Sagbadja H.,University Abomey Calavi | Diarrassouba N.,University Peleforo Gon Coulibaly Of Korhogo Ufr Des Science Biologiques | Sina H.,University Abomey Calavi | And 7 more authors.
Biochemistry Research International | Year: 2015

Kola nut is chewed in many West African cultures and is used ceremonially. The aim of this study is to investigate some biological effects of Cola nitida's bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusion method. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent phytochemicals. For the reference and meat isolated strains, the inhibitory diameter zone was from 17.5 ± 0.7 mm (C. albicans) to 9.5 ± 0.7 mm (P. vulgaris). The MIC ranged from 0.312 mg/mL to 5.000 mg/mL and the MBC from 0.625 mg/mL to >20 mg/mL. The highest antifungal activity was observed with F. verticillioides and the lowest one with P. citrinum. The two extracts have an excellent reducing free radical activity. The killing effect of A. salina larvae was perceptible at 1.04 mg/mL. The purified extracts of Cola nitida's bark can be used to hold meat products and also like phytomedicine. © 2015 Durand Dah-Nouvlessounon et al. Source

Kossouoh C.,Laboratoire Of Pharmacognosie Et Des Huiles Essentielles | Kossouoh C.,University Abomey Calavi | Moudachirou M.,Laboratoire Of Pharmacognosie Et Des Huiles Essentielles | Adjakidje V.,University Abomey Calavi | And 3 more authors.
Journal of Essential Oil-Bearing Plants | Year: 2011

Essential oils from fresh leaves of Chromolaena odorata (L.) were extracted by steam distillation. The oil from leaves (I) was obtained with a very low percentage. The extraction yield for the leaves (II) was 0.07 %. Analysis made by GC and GC-MS showed a total of 64 compounds identified. The main components of the oils were pregeijerene (29.9 %), germacrene D (21.6 %), β-caryophyllene (14.3 %), geijerene (10.1 %), α-pinene (8.0 %), sabinene (5.4 %). © 2011, Har Krishan Bhalla & Sons. Source

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