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Souho T.,University Sidi Mohammed Ben Abdellah | Fatemi H.E.,Laboratoire Central dAnalyse Medicale | Karim S.,University Sidi Mohammed Ben Abdellah | Rhazi K.E.,Laboratoire depidemiologie | And 6 more authors.
PLoS ONE | Year: 2016

Objectives To determine the distribution of cervical high-risk human papillomavirus genotypes and their association to cellular abnormalities in women from Fez and its neighborhood. Methods Women attending the Hassan II University Hospital for cervical pap smears were recruited after an informed consent. Interviews and two cervical samples were performed for each woman. Cervical samples were used for cytological analysis and HPV DNA detection. HPV was typed using a method based on multiplex PCR with fluorescently labeled specific primers followed by capillary electrophoresis. The study was approved by the ethics committee of the Faculty of Medicine and Pharmacy of Fez. Results The HPV prevalence in the studied population was 43.1% and the most prevalent types were HPV 53 (23 cases); HPV 16 (20 cases); HPV 35 (18 cases); HPV 51 (10 cases) and HPV 56 (7 cases). From the 619 confirmed pap smears, 20% were abnormal. The cytological abnormalities were significantly associated to HPV infection, women age, number of pregnancies and parity (p < 0.05). Conclusion More attention should be given to HPV in Morocco because it represents an important public health concern. The distribution of carcinogenic HPV types in the studied population is different from the data in other regions but epidemiological studies in other Moroccan regions are required. © 2016 Souho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

El Khadir M.,University Sidi Mohammed Ben Abdellah | Alaoui Boukhris S.,University Sidi Mohammed Ben Abdellah | Benajah D.-A.,Service DHepato Gastro enterologie CHU Hassan II de Fes | Benajah D.-A.,Laboratoire Of Pathologie Humaine | And 11 more authors.
Journal of the Chinese Medical Association | Year: 2016

Background: Finding a simple, accurate, and noninvasive diagnosis method is a substantial challenge for the detection of . Helicobacter pylori. The aim of the present study was to compare the presence of . H. pylori urease antigen in saliva with the presence of this bacterium in gastric mucosa. Methods: Saliva samples and gastric biopsies were taken from 153 consenting Moroccan patients. Saliva samples were analyzed using an immunochromatographic test for urease antigen . H. pylori detection. Thereafter, the gastric biopsies were analyzed by histology and polymerase chain reaction (PCR) to detect this bacterium. Results: From a total of 153 recruited Moroccan patients, . H. pylori was detected in 28 (18.30%), 87 (57.24%), and 69 (45.10%) cases by saliva test, histology, and PCR, respectively. A significant association was observed between the presence of . H. pylori antigen in saliva and age. However, no association was found with sex, . H. pylori virulence factors, gastric disease outcome, and density of the bacterium on the gastric mucosa. Considering that only 90 patients presented concordant results on . H. pylori diagnosis (positive or negative) by both histology and PCR, the immunochromatographic test showed very low sensitivity (29.79%) and high specificity (90.70%). Of these two tests, the positive and negative predictive values were 77.78% and 54.17%, respectively. The accuracy of the test for salivary detection of urease antigen . H. pylori was 58.89%. Conclusion: This study demonstrated a low detection rate of . H. pylori antigens in saliva compared with the presence of this bacterium in gastric mucosa, suggesting that saliva cannot be used as a suitable sample for the diagnosis of . H. pylori in our study population. © 2016. Source

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