Cecos Laboratoire Of Biologie Of La Reproduction

Marseille, France

Cecos Laboratoire Of Biologie Of La Reproduction

Marseille, France

Time filter

Source Type

Greco F.,Aix - Marseille University | Courbiere B.,Aix - Marseille University | Rose J.,Aix - Marseille University | Orsiere T.,Aix - Marseille University | And 5 more authors.
Gynecologie Obstetrique Fertilite | Year: 2015

Nanoparticles (NPs) are sized between 1 and 100nm. Their size allows new nanoscale properties of particular interest for industrial and scientific purpose. Over the past twenty years, nanotechnology conquered many areas of use (electronic, cosmetic, textile...). While, human is exposed to an increasing number of nanoparticles sources, health impacts and, particularly on reproductive function, remains poorly evaluated. Indeed, traceability of nanoparticles use is lacking and nanotoxicology follows different rules than classical toxicology. This review focuses on the impact of NPs on health and particularly on fertility and addresses potential risks of chronic exposure to NPs on human fertility. © 2014 Elsevier Masson SAS.


Aye M.,Jean Moulin University Lyon 3 | Di Giorgio C.,Jean Moulin University Lyon 3 | De Mo M.,Jean Moulin University Lyon 3 | Botta A.,Jean Moulin University Lyon 3 | And 4 more authors.
Food and Chemical Toxicology | Year: 2010

Vitrification requires high concentrations of cryoprotectants that may induce long-term toxic effects on cells. The aim of this study was to evaluate the possible genotoxicity of three cryoprotectants extensively used for oocyte vitrification: dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PROH). For this purpose, a Chinese Hamster Ovary cell line (CHO), commonly used in genetic toxicology, was selected as an in vitro biological model to assess both the induction of DNA strand-breaks as identifiable by the alkaline comet assay and the persistence of chromosomal damages (micronuclei) as analyzed by the micronucleus assay. Results showed that DMSO was not genotoxic. EG did not exert direct genotoxic activity, however EG exhibited significant genotoxic and clastogenic activities in the presence of an external cytochrome-based P450 oxidation system (S9 Mix). PrOH produced in vitro DNA-damage leading to chromosome mutations in the presence and absence of the S9 Mix. These results showed that high concentrations of EG and PrOH could induce in vitro chromosomal damage in eukaryotic cells. © 2010.


Chabert M.-C.,Aix - Marseille University | Perrin J.,Pole Femmes Meres Enfants | Perrin J.,Aix - Marseille University | Perrin J.,Cecos Laboratoire Of Biologie Of La Reproduction | And 6 more authors.
European Journal of Obstetrics Gynecology and Reproductive Biology | Year: 2016

Objective The aim of this study was to evaluate the information pregnant women received regarding possible exposures to five recognized reprotoxic agents during their pregnancy. Study design A cohort study was conducted using two postnatal units in France. Women hospitalized in postnatal units were requested to complete a self-administered two part questionnaire. The first part gathered information about the patient's socio-professional level and the type of pregnancy follow-up. The second part examined the information the patient received regarding daily products containing the following known reprotoxic agents: bisphenol A, toluene, n-hexane, cis-chloroallyl-triaza-azonia-adamantane-chloride and O-phenyl-phenol. The women cited the sources of information. We combined the employment status and educational level to separate the women into two groups. The groups were then compared using the Chi Square test or Fisher's exact test. Result(s) There were 390 women in this study. Our results showed the women received information regarding the following: 21.6% (n = 84) regarding tin cans, 21.9% (n = 85) concerning plastic meal boxes when heated in microwave ovens, 8.8% (n = 32) about water in gas-bottles, 27.4% (n = 106) about non-organic foods, 39.3% (n = 152) about hair dyes, 17% (n = 66) about nail polishes, 23.4% (n = 103) about insect repellents, 34.4% (n = 133) about “do-it-yourself” products, 2.1% (n = 8) about gardening products, 26.7% (n = 103) about electric plug-in repellents, 21.1% (n = 81) about housekeeping products, and 6.8% (n = 26) about register receipts. Women with a higher level of education and a qualified occupation were better informed about these daily products. These women were more likely to learn the information on their own (internet, media). Conclusion(s) Our study showed French women did not receive sufficient information regarding potential exposures to reprotoxic agents during pregnancy. © 2016 Elsevier Ireland Ltd


Perrin J.,Cecos Laboratoire Of Biologie Of La Reproduction | Perrin J.,Aix - Marseille University | Tassistro V.,Aix - Marseille University | Mandon M.,Aix - Marseille University | And 6 more authors.
Fertility and Sterility | Year: 2011

Objective: To analyze the distribution of benzo(a)pyrene-diol-epoxide (BPDE)-DNA adducts in spermatozoa selected and nonselected by a swim-up procedure with relation to smoking habits. Design: Comparative study. Setting: Public university and public university hospital. Patient(s): Seventy-nine men (37 smokers and 42 nonsmokers) who visited an infertility clinic for diagnostic. Intervention(s): Tobacco and environmental exposure assessment, semen sample analysis, swim-up procedure, BPDE-DNA adduct immunolabeling. Main Outcome Measure(s): BPDE-DNA adduct quantification in selected (SEL-SPZ) and nonselected (NONSEL-SPZ) spermatozoa. Data were normalized by using a normalized fluorescence value (NFV). Result(s): The mean NFV (±SD) in SEL-SPZ was significantly higher in smokers than in nonsmokers (18.9 ± 11.5 vs. 10.5 ± 10.4, respectively). Within smokers, a paired analysis (SEL-SPZ and NONSEL-SPZ) showed that NFV was significantly lower in SEL-SPZ than in NONSEL-SPZ (20.0 ± 11.3 vs. 31.5 ± 16.0, respectively). Conversely, within nonsmokers, the mean NFV was higher in SEL-SPZ than in NONSEL-SPZ (10.3 ± 10.6 vs 4.3 ± 7.1, respectively). Conclusion(s): Tobacco consumption is associated with BPDE-DNA adducts in spermatozoa. In smokers, semen processing by swim-up recovers potentially fertilizing spermatozoa that show a significantly lower amount of BPDE-DNA adducts compared with NONSEL-SPZ. Further study is needed to improve the spermatozoa selection in smoking patients requiring assisted reproductive technologies. © 2011 by American Society for Reproductive Medicine.


Ould Hamouda S.,Service de Medecine et Sante Au Travail | Ould Hamouda S.,Cecos Laboratoire Of Biologie Of La Reproduction | Perrin J.,Cecos Laboratoire Of Biologie Of La Reproduction | Perrin J.,Center Clinico biologique dAssistance Medicale A la Procreation | And 11 more authors.
Journal de Gynecologie Obstetrique et Biologie de la Reproduction | Year: 2016

Alteration of sperm parameters related to occupational exposures is the subject of several studies, often on a case-control approach. The study populations usually comprise men consulting in infertility clinics for couple infertility. The objective of this review is to identify, from these case-control studies, the main occupational factors that may be associated with altered sperm parameters. We selected 13 articles in the PubMed database. Participation in these studies varied from 61 to 2619 subjects, with great methodological heterogeneity, particularly in the characterization of exposure. The main occupations that appear significantly associated with a risk of altered sperm parameters are workmen, painters, farmers, welders, plumbers and technicians. When analysis focuses on occupational exposures, a significant result is reported for solvents, heavy metals, heat, vibrations and non-ionizi. © 2015 Elsevier Masson SAS.


Roustan A.,Aix - Marseille University | Perrin J.,Aix - Marseille University | Perrin J.,Cecos Laboratoire Of Biologie Of La Reproduction | Berthelot-Ricou A.,Aix - Marseille University | And 3 more authors.
Laboratory Animals | Year: 2012

Cervical dislocation is a commonly used method of mouse euthanasia. Euthanasia by isoflurane inhalation is an alternative method which allows the sacrifice of several mice at the same time with an anaesthesia, in the aim to decrease pain and animal distress. The objective of our study was to assess the impact of these two methods of euthanasia on the quality of mouse oocytes. By administering gonadotropins, we induced a superovulation in CD1 female mice. Mice were randomly assigned to euthanasia with cervical dislocation and isoflurane inhalation. Oviducts were collected and excised to retrieve metaphase II oocytes. After microscopic examination, oocytes were classified into three groups: intact, fragmented/cleaved and atretic. Intact metaphase II oocytes were employed for biomedical research. A total of 1442 oocytes in the cervical dislocation group were compared with 1230 oocytes in the isoflurane group. In the cervical dislocation group, 93.1% of the oocytes were intact, versus 65.8% in the isoflurane group (P ≤ 0.001). In light of these results, we conclude that cervical dislocation is the best method of mouse euthanasia for obtaining intact oocytes for biomedical research.


PubMed | Aix - Marseille University and Cecos Laboratoire Of Biologie Of La Reproduction
Type: Journal Article | Journal: Journal de gynecologie, obstetrique et biologie de la reproduction | Year: 2016

Alteration of sperm parameters related to occupational exposures is the subject of several studies, often on a case-control approach. The study populations usually comprise men consulting in infertility clinics for couple infertility. The objective of this review is to identify, from these case-control studies, the main occupational factors that may be associated with altered sperm parameters. We selected 13 articles in the PubMed database. Participation in these studies varied from 61 to 2619 subjects, with great methodological heterogeneity, particularly in the characterization of exposure. The main occupations that appear significantly associated with a risk of altered sperm parameters are workmen, painters, farmers, welders, plumbers and technicians. When analysis focuses on occupational exposures, a significant result is reported for solvents, heavy metals, heat, vibrations and non-ionizing radiation. None of the selected studies has found a link with exposure to pesticides.


PubMed | Cecos Laboratoire Of Biologie Of La Reproduction
Type: Comparative Study | Journal: Fertility and sterility | Year: 2011

To analyze the distribution of benzo(a)pyrene-diol-epoxide (BPDE)-DNA adducts in spermatozoa selected and nonselected by a swim-up procedure with relation to smoking habits.Comparative study.Public university and public university hospital.Seventy-nine men (37 smokers and 42 nonsmokers) who visited an infertility clinic for diagnostic.Tobacco and environmental exposure assessment, semen sample analysis, swim-up procedure, BPDE-DNA adduct immunolabeling.BPDE-DNA adduct quantification in selected (SEL-SPZ) and nonselected (NONSEL-SPZ) spermatozoa. Data were normalized by using a normalized fluorescence value (NFV).The mean NFV (SD) in SEL-SPZ was significantly higher in smokers than in nonsmokers (18.911.5 vs. 10.510.4, respectively). Within smokers, a paired analysis (SEL-SPZ and NONSEL-SPZ) showed that NFV was significantly lower in SEL-SPZ than in NONSEL-SPZ (20.011.3 vs. 31.516.0, respectively). Conversely, within nonsmokers, the mean NFV was higher in SEL-SPZ than in NONSEL-SPZ (10.310.6 vs 4.37.1, respectively).Tobacco consumption is associated with BPDE-DNA adducts in spermatozoa. In smokers, semen processing by swim-up recovers potentially fertilizing spermatozoa that show a significantly lower amount of BPDE-DNA adducts compared with NONSEL-SPZ. Further study is needed to improve the spermatozoa selection in smoking patients requiring assisted reproductive technologies.

Loading Cecos Laboratoire Of Biologie Of La Reproduction collaborators
Loading Cecos Laboratoire Of Biologie Of La Reproduction collaborators