Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique

Sfax, Tunisia

Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique

Sfax, Tunisia
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Khlif M.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Bogreau H.,Institute Of Medecine Tropicale Du Service Of Sante Des Armees | Michel-Nguyen A.,Aix - Marseille University | Ayadi A.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Ranque S.,Aix - Marseille University
Antimicrobial Agents and Chemotherapy | Year: 2010

Multilocus microsatellite polymorphisms in 27 clinical Candida albicans isolates were found to be clearly unrelated to in vitro paradoxical growth or trailing effect with caspofungin. These findings suggest that such in vitro phenotypes are either gained or lost too rapidly to be predicted by more stable genomic markers. Copyright © 2010, American Society for Microbiology. All Rights Reserved.


Hadrich I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Ranque S.,Aix - Marseille University | Ranque S.,Marseille University Hospital Center
Current Fungal Infection Reports | Year: 2015

Typing is applied to highlight the genetic relationships between environmental and clinical fungal isolates involved in colonization or infection. A variety of techniques can be used to type fungi, depending on the epidemiological question and the available equipment. The use of typing techniques during clinical fungal outbreak investigations demonstrated patient-to-patient propagation in dermatophytoses outbreaks, patient-to-health-care worker and health-care worker-to-patient transmission in yeast infection outbreaks, airborne patient-to-patient transmission of the non-cultivable Pneumocystis jirovecii fungus involved in pneumonia outbreaks, and environmental sources of several mold outbreaks including aspergillosis or fusariosis keratitis. Typing was also useful to trace the source of invasive fungal disease outbreaks or epidemics, such as contaminated medical devices. More generally, typing provided important insights into fungal outbreak characteristics and helped to implement appropriate measures to control fungal infections, which are often severe, progress rapidly, and are difficult to diagnose and treat. © 2015, Springer Science+Business Media New York.


Hadrich I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Neji S.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Drira I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Trabelsi H.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | And 4 more authors.
Medical Mycology | Year: 2013

Aspergillus flavus is the second most important Aspergillus species associated with aspergillosis and the incidence of infections caused by it are increasing in the immunocompromised population. This species is of major epidemiological importance in regions with a dry and hot climate. Despite the growing clinical significance of A. flavus, data on its molecular epidemiology are scarce. This study was aimed at examining whether isolates from distinct genotypes were involved in distinct clinical forms of aspergillosis. Sixty-three clinical isolates of A. flavus recovered from 35 patients with various clinical presentations of aspergillosis were characterized by microsatellite typing. The highest discriminatory power for a single locus was obtained with the AFLA1 marker, which had 14 distinct alleles and a 0.903 D value. The combination of all six markers yielded 48 different genotypes with a 0.994 D value. There was a considerable genetic diversity in the isolates and patients with invasive aspergillosis were usually colonized by multiples genotypes. There was no evidence that a given genotype was associated with a particular clinical presentation of A. flavus aspergillosis. The occurrence of more than one genotype in clinical samples indicates that a patient may be infected by multiple genotypes and that any particular isolate from a clinical specimen may not necessarily be the one causing aspergillosis. © 2013 ISHAM.


Hadrich I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Makni F.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Ayadi A.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Ranque S.,Aix - Marseille University
Journal of Clinical Microbiology | Year: 2010

Assessing the relatedness of strains isolated from patients and their environment is instrumental in documenting the source of preventable health care-associated life-threatening Aspergillus flavus human infection clusters. The present study aimed at identifying and selecting suitable microsatellite markers for A. flavus typing. This typing scheme was then applied to investigate the A. flavus epidemiology within a hematology unit in Sfax, Tunisia. Use of a combination of five markers made it possible to discern clusters of isolates and to substantiate the genetic diversity of A. flavus within clusters. Isolates from Tunisia and Marseille, France, displayed distinct haplotypes, indicating a highly significant geographical structuring of A. flavus. The typing of clinical and environmental A. flavus isolates in a hematology unit provided insights into its hospital epidemiology. From a heterogeneous genetic background, a cluster indicative of a clonal propagation episode within the unit could be identified. In two patients with invasive aspergillosis, the same genotype was found in clinical and environmental isolates, indicating hospital-acquired colonization and infection. In further studies, this novel microsatellite typing scheme might be instrumental in illuminating important epidemiological issues about A. flavus population genetics or epidemiology, including tracing the sources and routes of transmission. Copyright © 2010, American Society for Microbiology. All Rights Reserved.


Hadrich I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Neji S.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Makni F.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Ayadi A.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | And 3 more authors.
Journal of Medical Microbiology | Year: 2014

There are limited data on in vitro susceptibility testing of echinocandins against Aspergillus species. The objective of this study was to describe the phenotypes of Aspergillus flavus observed on exposure to caspofungin in vitro and to test whether these phenotypes were associated with A. flavus genotypes. The caspofungin MICs of 37 A. flavus clinical isolates collected from 14 patients with invasive aspergillosis were determined using Etest assays. Caspofungin MICs ranged from 0.012 to 0.064 mg l-1; the modal MIC was 0.023 mg l-1 and the MIC50 and MIC90 were 0.032 and 0.064 mg l-1, respectively. A clear end point was noted in 24 (65%) isolates, whereas seven (19%) displayed a trailing effect and six (16%) showed paradoxical growth when exposed to caspofungin. In these A. flavus isolates, the absence of a significant population structure or genetic differentiation indicated that trailing or paradoxical growth phenotypes were independent of microsatellite genotype. © 2014 The Authors.


Mseddi F.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Sellami A.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Sellami H.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Cheikhrouhou F.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | And 2 more authors.
Mycoses | Year: 2011

Cryptococcus neoformans is an encapsulated yeast-like fungus that causes life-threatening infections, particularly in immunocompromised patients. The formation of brown pigment on many media described in the literature, such as that in Niger seed (Guizotia abyssinica) agar, has been used to identify C. neoformans. The present study compares melanin production by clinical and environmental isolates of C. neoformans and other medically important yeast on two new media, Pinus halepensis seed (PHS) agar and blackberry (BlaB) agar, and the classic medium Niger seed agar. Results obtained after the culture of 46 strains of C. neoformans, for 4, 24 and 48h at 37°C on these three media, showed that at 24h, 100% of strains were pigmented on BlaB agar, 91.3% on PHS agar but only 34.8% on Niger seed agar. In conclusion, PHS and BlaB agar are two interesting new media for the rapid identification of C. neoformans isolates. © 2010 Blackwell Verlag GmbH.


Hadrich I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Amouri I.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Neji S.,Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique | Mahfoud N.,Service du Laboratoire | And 4 more authors.
European Journal of Clinical Microbiology and Infectious Diseases | Year: 2013

Aspergillus flavus is the second leading cause of allergic, invasive, and colonizing fungal diseases in humans, and also the second most frequent organism associated with avian infections. Currently, it is not known whether there is a link between the environmental isolates and/or human isolates of A. flavus and those responsible for aspergillosis in birds. Microsatellite typing was used to analyze 29 A. flavus clinical and environmental avian isolates and 63 human clinical isolates collected from patients with a variety of aspergillosis diseases. The combination of all six markers yielded 77 different genotypes with a 0.98 D value. A. flavus genotypes obtained from avian isolates were compared with those obtained from human clinical and environmental samples. The standardized indices of association I A and rBarD were significantly different from zero (p < 0.01), suggesting a prevailing clonal reproduction. There was high genetic diversity between the hospital and poultry environments of A. flavus isolates. The human environmental population was significantly differentiated from environmental and clinical avian populations (F st > 0.25). The avian clinical subpopulation exchanged few strains with the environmental human (N m = 7.24) and avian (N m = 6.60) populations. The minimum spanning tree analysis identified three A. flavus genotype clusters that were highly structured according to the isolation source (p < 10-4). © 2012 Springer-Verlag.


PubMed | Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique
Type: Journal Article | Journal: Medical mycology | Year: 2013

Aspergillus flavus is the second most important Aspergillus species associated with aspergillosis and the incidence of infections caused by it are increasing in the immunocompromised population. This species is of major epidemiological importance in regions with a dry and hot climate. Despite the growing clinical significance of A. flavus, data on its molecular epidemiology are scarce. This study was aimed at examining whether isolates from distinct genotypes were involved in distinct clinical forms of aspergillosis. Sixty-three clinical isolates of A. flavus recovered from 35 patients with various clinical presentations of aspergillosis were characterized by microsatellite typing. The highest discriminatory power for a single locus was obtained with the AFLA1 marker, which had 14 distinct alleles and a 0.903 D value. The combination of all six markers yielded 48 different genotypes with a 0.994 D value. There was a considerable genetic diversity in the isolates and patients with invasive aspergillosis were usually colonized by multiples genotypes. There was no evidence that a given genotype was associated with a particular clinical presentation of A. flavus aspergillosis. The occurrence of more than one genotype in clinical samples indicates that a patient may be infected by multiple genotypes and that any particular isolate from a clinical specimen may not necessarily be the one causing aspergillosis.


PubMed | Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique
Type: Comparative Study | Journal: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology | Year: 2013

Aspergillus flavus is the second leading cause of allergic, invasive, and colonizing fungal diseases in humans, and also the second most frequent organism associated with avian infections. Currently, it is not known whether there is a link between the environmental isolates and/or human isolates of A. flavus and those responsible for aspergillosis in birds. Microsatellite typing was used to analyze 29 A. flavus clinical and environmental avian isolates and 63 human clinical isolates collected from patients with a variety of aspergillosis diseases. The combination of all six markers yielded 77 different genotypes with a 0.98 D value. A. flavus genotypes obtained from avian isolates were compared with those obtained from human clinical and environmental samples. The standardized indices of association I (A) and rBarD were significantly different from zero (p<0.01), suggesting a prevailing clonal reproduction. There was high genetic diversity between the hospital and poultry environments of A. flavus isolates. The human environmental population was significantly differentiated from environmental and clinical avian populations (F (st)>0.25). The avian clinical subpopulation exchanged few strains with the environmental human (N (m)=7.24) and avian (N (m)=6.60) populations. The minimum spanning tree analysis identified three A. flavus genotype clusters that were highly structured according to the isolation source (p<10(-4)).


PubMed | Laboratoire Of Biologie Moleculaire Parasitaire Et Fongique
Type: Journal Article | Journal: Journal of medical microbiology | Year: 2012

Aspergillosis is one of the most common causes of death in captive birds. Aspergillus fumigatus accounts for approximately 95 % of aspergillosis cases and Aspergillus flavus is the second most frequent organism associated with avian infections. In the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). Microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of A. flavus. A. flavus was the only species (28 % prevalence) detected in the avian clinical isolates, whereas this species ranked third (19 %) after members of the genera Penicillium (39 %) and Cladosporium (21 %) in the environmental samples. Upon microsatellite analysis, five to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a 0.852 D value. The combination of all six markers yielded a 0.991 D value with 25 distinct genotypes. One clinical avian isolate (lung biopsy) and one environmental isolate (egg) shared the same genotype. Microsatellite typing of A. flavus grown from avian and environmental samples displayed an excellent discriminatory power and 100 % reproducibility. This study showed a clustering of clinical and environmental isolates, which were clearly separated. Based upon these results, aspergillosis in birds may be induced by a great diversity of isolates.

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