Laboratoire Of Biologie Moleculaire Et Of Genie Genetique

Liège, Belgium

Laboratoire Of Biologie Moleculaire Et Of Genie Genetique

Liège, Belgium
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Vreuls C.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | Zocchi G.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | Thierry B.,University of South Australia | Garitte G.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | And 5 more authors.
Journal of Materials Chemistry | Year: 2010

In this study, robust antibacterial coatings were created on stainless steel through the covalent grafting of antibacterial peptides onto an organic-polymeric interlayer deposited by RF-glow discharge plasma. X-Ray photoelectron spectroscopy was used to characterize and optimize the two steps of the coating process. The biocidal activity of these surfaces was demonstrated against both Gram+ and Gram- bacteria using ISO tests. 3 to 6 log10 reductions of both Gram+ and Gram- bacterial strains were obtained compared to uncoated stainless steel and depending on the particular antibacterial peptide immobilized. Importantly the antibacterial surfaces were resistant to several cleaning conditions. The latter is significant as the stability of such antibacterial surfaces in close to real life conditions is a major concern and leaching, de-lamination, rearrangement and ageing of the coating can lead to insufficient long term biofilm resistance of the surface. © 2010 The Royal Society of Chemistry.


Vreuls C.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | Zocchi G.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | Genin A.,Laboratoire Of Biologie Moleculaire Et Of Genie Genetique | Archambeau C.,ArcelorMittal | And 2 more authors.
Journal of Inorganic Biochemistry | Year: 2010

This paper highlights an innovative application of inorganic-binding peptides as quality control tools for detecting defects on inorganic surfaces of any shape. The approach involves attaching a fluorescent label to an inorganic-binding peptide and exploiting the peptide's high binding specificity to detect, by simple fluorescence microscopy, chemical composition defects of μm size and crystallographic state defects. Proof of concept was demonstrated by monitoring binding of a previously isolated ZnO-binding peptide to galvanized steel substrates. The approach was further validated for TiO 2 coatings and stainless steel, with two new, specific inorganic-binding peptides isolated by phage display. © 2010 Elsevier Inc.

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