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Kouidhi B.,University of Monastir | Zmantar T.,University of Monastir | Hentati H.,University of Monastir | Najjari F.,University of Monastir | And 2 more authors.
Archives of Oral Biology | Year: 2011

Objective: This study aims to investigate the antibiotic susceptibility of strains isolated from the oral cavity of Tunisian children. Design: Strains were isolated from the oral cavity of Tunisian children (60 caries-actives and 30 caries-free). Molecular characterization was assessed by PCR assay to detect erythromycin methylase gene (ermB), macrolide efflux (mefI) and tetracycline resistance genes (tetM and tetO). Results: A total of 21 species were isolated and identified. Antimicrobial susceptibility revealed that the resistance rate to antibiotics was as follow: erythromycin (22%), tetracycline (15.6%), cefotaxim, (7.3%), trimethoprim-sulfamethoxazol (37.6%), nitrofurantoine (2.8%), pristinamycin (17.4%), quinupristin-dalfopristin (15.6%), and rifampicin (3.7%). The majority of mefI positive strains (31.2%) were isolated from the carious children (n = 34) in comparison with 8.25% from the control group (n = 9). In addition, frequency of strains caring resistance genes were as follow: 12.84% for ermB, 9.17% for tetM and 27.52% for tetO from the carious children in comparison to 0.092%, 3.67% and 3.67% from the caries free group respectively. Conclusion: Multi-resistance strains towards macrolides and tetracycline were recorded. The majority of strains carrying antibiotics resistance genes were isolated from the caries active children. The presence of multi-resistant bacteria in the oral cavity can be the major cause of antibiotic prophylaxis failure in dental practise. © 2010 Elsevier Ltd. All rights reserved.

Silvestre A.,Center Dimagerie Osteo Articulaire | Peuchant A.,Laboratoire Of Biologie Moleculaire | Bausset O.,Marseille University Hospital Center | Magalon J.,Marseille University Hospital Center | And 2 more authors.
Journal de Traumatologie du Sport | Year: 2014

Background: Achilles tendinopathy remains often chronic and rebel to a thorough conservative treatments. In this context, autologous platelet-rich plasma (PRP) could represent an interesting alternative. Methods: This prospective, monocentric, descriptive study, conducted from January 2011 to May 2011, included 32 patients practicing sports at the competitive or amateur level and suffering from chronic Achilles tendinopathy diagnosed by ultrasound (US). The treatment consisted on a single injection of PRP directly into the Achilles tendon under US guidance. Hence, the cracks were gently filled without damaging the surrounding healthy tendon. PRP was systematically characterized before its administration. Tendon regeneration was followed using algofunctionnal testing and US examination at 1, 2, and 3. months post-injection. A satisfaction score was proposed at 6 and 12. months by phone call. Results: Twenty-two patients (69%) at 1. month and 18patients (88%) at 2. months were completely healed, associated with a widespread improvement of fibrillar echo-structure of the tendon and improvement reduction of the hypervascularity during power Doppler examination at 3. months. Of the 22 responses collected to the end of the protocol, 14 patients declared a complete improvement in term of satisfaction with a significant reduction of pain (P < 0.05), 4 patients reported a significant reduction of pain more than 50% (P < 0.05). Only 4 patients did not respond to the treatment and were secondary operated. Conclusions: A single autologous PRP injection was effective in the treatment of rebel Achilles tendinopathy. US guidance allows PRP injection directly into the degenerated part of the tendon with great accuracy. © 2014 Elsevier Masson SAS.

Kouidhi B.,University of Monastir | Zmantar T.,University of Monastir | Mahdouani K.,Laboratoire Of Biologie Moleculaire | Hentati H.,University of Monastir | Bakhrouf A.,University of Monastir
BMC Microbiology | Year: 2011

Abstract. Background: Enterococci are increasingly associated with opportunistic infections in Humans but the role of the oral cavity as a reservoir for this species is unclear. This study aimed to explore the carriage rate of Enterococci in the oral cavity of Tunisian children and their antimicrobial susceptibility to a broad range of antibiotics together with their adherence ability to abiotic and biotic surfaces. Results: In this study, 17 E. faecalis (27.5%) and 4 E. faecium (6.5%) were detected. The identified strains showed resistance to commonly used antibiotics. Among the 17 isolated E. faecalis, 12 strains (71%) were slime producers and 5 strains were non-producers. Among the 4 E. faecium, 2 strains were slime producers. All the tested strains were able to adhere to at least one of the two tested cell lines. Our result showed that 11 E. faecalis and 2 E. faecium strains adhered strongly to Hep-2 as well as to A549 cells. Conclusions: Drugs resistance and strong biofilm production abilities together with a high phenotypic adhesion to host cells are important equipment in E. faecalis and E. faecium which lead to their oral cavity colonization and focal infections. © 2011 Kouidhi et al; licensee BioMed Central Ltd.

Souiden Y.,Laboratoire Of Biologie Moleculaire | Bouraoui A.,University of Monastir | Chaieb K.,Laboratoire Of Biologie Moleculaire | Mahdouani K.,Laboratoire Of Biologie Moleculaire
Bulletin du Cancer | Year: 2010

Advances in chromosome dynamics have increased our understanding of the significant role of telomeres and telomerase in cancer. Telomerase is expressed in almost all cancer cells but is inactive in most normal somatic cells. Therefore, telomerase is an important target for the design of therapeutic agents that might have minimal side effects. Herein, we evaluate current approaches to telomerase/telomere-targeted therapy, discuss the benefits and disadvantages, and speculate on the future direction of telomerase inhibitors as cancer therapeutics. ©John Libbey Eurotext.

Mrad M.,Tunis el Manar University | Mrad M.,Laboratoire Of Biologie Moleculaire | Wathek C.,Tunis el Manar University | Saleh M.B.,Tunis el Manar University | And 7 more authors.
Transfusion and Apheresis Science | Year: 2014

The role of two polymorphisms C677T and A1298C of the methylenetetrahydrofolate reductase (MTHFR) gene in the etiology of retinal vein occlusion (RVO) has not been adequately clarified. The aim of this study was to examine the prevalence of these polymorphisms among RVO Tunisian patients with and without systemic risk factors. Seventy-two patients with retinal vein occlusion (RVO) were studied. The control group included140 people matched for age, sex, and risk factors. Participants in the study were genotyped for the MTHFR C677T and A1298C polymorphisms. The genotyping was performed by PCR-RFLP. No significant differences were found in the frequencies of the three genotypes (AA, AC, CC) of the MTHFR A1298C polymorphism between RVO patients and healthy controls. However, the prevalence of the group of mutated genotypes (AC+CC) of the missense variant MTHFR A1298C was significantly different between patients and controls (16.67% vs. 6.42%, p=01). Additionally, the frequency of the CT genotype as well as the group of combined mutated genotypes (CT+TT) for the C677T variant was significantly higher among RVO patients compared with controls (p<10-3, p<10-3). This suggests an association between this polymorphism and RVO. Large study populations would be required to understand more completely the contribution of these markers in the risk of RVO. © 2014 Elsevier Ltd.

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