Laboratoire Of Biologie Et Genetique Moleculaire

Constantine, Algeria

Laboratoire Of Biologie Et Genetique Moleculaire

Constantine, Algeria
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Abbas A.,Laboratoire Of Biologie Et Genetique Moleculaire | Abbas A.,University of Mentouri Constantine | Sifi K.,Laboratoire Of Biologie Et Genetique Moleculaire | Sifi K.,Laboratoire Of Biochimie | And 5 more authors.
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016

Impairment of folate and homocysteine metabolism has been observed in mothers of fetuses with neural tube defects (NTDs) and infants affected; they are considered as risk factors. The novelty of this study is to investigate, in Algerian population, the contribution of two polymorphic variations in genes involved in the folate dependent homocysteine metabolism in the aetiology of neural tube defects. Methionine synthase (MTR) A2756G and methionine synthase reductase (MTRR) A66G variants have been identified using PCR-RFLP analysis on a group of 48 NTD infants, 38 mothers of NTD cases and 67 normal controls. The effects of mutant variants on total plasma homocysteine (tHcy), serum folate and RBC (red blood cell) folate concentrations have also been evaluated in studied mothers of NTD cases. The calculated odds ratios (OR) show that the polymorphism A2756G of the MTR gene was not associated with NTD risk for both mothers and NTD cases. It seems not to influence homocysteine or serum folate concentrations but it does for RBC folate. The mean RBC folate level was significantly less in mothers of NTD cases having AG genotype (148.25 ± 47.55 ng/ml) with respect to mothers harbouring AA genotype (403.78 ± 213.18 ng/ml) (p < 0.05). Thus, MTRA2756G mutation could disrupt folate metabolism in mothers of our population. MTRR A66G mutationhas no significant influence on NTD risk or on metabolites levels. © 2016, Global Research Online. All rights reserved.


Rezgoune M.L.,University of Mentouri Constantine | Chellat D.,Laboratoire Of Biologie Et Genetique Moleculaire | Abadi N.,University of Constantine 3 | Satta D.,Laboratoire Of Biologie Et Genetique Moleculaire | Mendjli N.V.A.,Ain el Bey
International Journal of Pharmaceutical Sciences Review and Research | Year: 2016

The purpose of this study was to investigate the association between MTHFR gene single nucleotide polymorphism A1298C and the risk of human male infertility in Algerian population. In this case-control study, peripheral venous blood was taken from 89 patients with spermatogenesis failure and 84 fertile controls for DNA extraction and kept in EDTA vacutainer tubes for molecular analysis. Controls were age-matched to cases. The genotyping was carried out using Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) and gel electrophoresis. Data were analyzed using Chi square test. There was no significant difference in genotype frequency of A1298C between cases and controls [cases: AA (59.55 %), AC (38.20 %) and CC (2.25 %); controls: AA (51.19 %), AC (47.62 %) and CC (1.19 %); p= 0.84]. There is no evidence for an association between male infertility and MTHFR 1298C polymorphism in the Algerian population. Future studies with a larger sample size are needed to validate our findings. © 2016, Global Research Online. All rights reserved.


Chellat D.,Laboratoire Of Biologie Et Genetique Moleculaire | Rezgoune M.L.,Laboratoire Of Biologie Et Genetique Moleculaire | Hamane D.,Sina | Abadi N.,University of Constantine 3 | Satta D.,Laboratoire Of Biologie Et Genetique Moleculaire
International Journal of Pharmaceutical Sciences Review and Research | Year: 2015

In this study we describe clinical and cytogenetic findings in a male referred to our laboratory for cytogenetic investigation. Karyotyping was performed on peripheral blood lymphocytes according to standard methods. Genomic DNA was isolated from blood and PCR was carried out with a set of 6 AZFa, AZFb and AZFc STS markers to detect the Y microdeletions. The patient’s age was 36 at the time of referral. Severe oligoasthenoteratozoospermia was confirmed on semen analysis. The karyotype of peripheral blood showed 46, XY, ins(1;2) (p31; p13p23). To the best of our knowledge this is only the four report in literature of infertile patient with chromosomal insertion but, there is no previous report of this specific chromosome rearrangement. Such an autosomal anomaly may lead to the disruption of genes responsible for spermatogenesis or impaired synaptic complex pairing during meiosis resulting in reproductive failure. © 2015, Global Research Online. All rights reserved.


Abbas A.,Laboratoire Of Biologie Et Genetique Moleculaire | Abbas A.,University of Mentouri Constantine | Abadi N.,Laboratoire Of Biologie Et Genetique Moleculaire | Abadi N.,Laboratoire Of Biochimie | And 6 more authors.
Immuno-Analyse et Biologie Specialisee | Year: 2012

The common single nucleotide polymorphism (SNP) C677. T in the MTHFR gene is the first genetic risk factor of neural tube defects (NTD). It has been shown to be present, in increased frequency, in some studies in patients having neural tube defects and their mothers. This study objective is to research the C677. T mutation in mothers of children having NTDs in Constantine population, and to evaluate its impact on folate and homocysteine concentrations. The study included 27 mothers of NTD patients and a control group of 46 mothers. The mutation was determined by polymerase chain reaction - restriction fragment length polymorphism analysis. Plasma homocysteine, serum folate and erythrocyte folate concentrations were analyzed using an automated chemiluminescence method. Results have shown that C677. T genotype frequencies in NTD mothers were not significantly different from those of controls. However, they showed that plasma Hcy levels were significantly higher in mothers of children with NTDs compared to controls; 13,75 ± 10,7 μmol/l in mothers versus 7,67 ± 3,83. μmol/l in controls (p < 0,01). This elevation was associated, principally, to the mutation. Through their effect on homocysteine, MTHFR mutation can be implicated as a risk factor for NTDs in our population. © 2012 Elsevier Masson SAS.

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