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Meklat A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Meklat A.,Blida University | Bouras N.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Riba A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | And 7 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2014

A halophilic actinomycete strain, designated H27T, was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10-15 % (w/v) and the optimum growth temperature and pH were found to be 28-37 °C and 6-7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27T were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27T are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27 T is most closely related to Streptomonospora alba DSM 44588 T (98.8 %) and Streptomonospora flavalba DSM 45155T (98.7 %) whereas the DNA-DNA relatedness values between strain H27T and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27T should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27 T (=DSM 45604T =CCUG 63369T =MTCC 11563 T). © 2014 Springer International Publishing. Source


Goudjal Y.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Toumatia O.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Sabaou N.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Barakate M.,Cadi Ayyad University | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2013

Twenty-seven endophytic actinomycete strains were isolated from five spontaneous plants well adapted to the poor sandy soil and arid climatic conditions of the Algerian Sahara. Morphological and chemotaxonomical analysis indicated that twenty-two isolates belonged to the Streptomyces genus and the remaining five were non-Streptomyces. All endophytic strains were screened for their ability to produce indole-3-acetic acid (IAA) in vitro on a chemically defined medium. Eighteen strains were able to produce IAA and the maximum production occurred with the Streptomyces sp. PT2 strain. The IAA produced was further extracted, partially purified and confirmed by thin layer chromatography (TLC) analysis. The 16S rDNA sequence analysis and phylogenetic studies indicated that strain PT2 was closely related to Streptomyces enissocaecilis NRRL B 16365T, Streptomyces rochei NBRC 12908T and Streptomyces plicatus NBRC 13071T, with 99.52 % similarity. The production of IAA was affected by cultural conditions such as temperature, pH, incubation period and l-tryptophan concentration. The highest level of IAA production (127 μg/ml) was obtained by cultivating the Streptomyces sp. PT2 strain in yeast extract-tryptone broth supplemented with 5 mg l-tryptophan/ml at pH 7 and incubated on a rotary shaker (200 rpm) at 30 °C for 5 days. Twenty-four-hour treatment of tomato cv. Marmande seeds with the supernatant culture of Streptomyces sp. PT2 that contained the crude IAA showed the maximum effect in promoting seed germination and root elongation. © 2013 Springer Science+Business Media Dordrecht. Source


Meklat A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Meklat A.,Blida University | Bouras N.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Zitouni A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | And 7 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

A novel halophilic actinomycete strain, H23T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23T had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136T) to 94.8 % (Actinopolyspora mortivallis DSM 44261T). The strain grew optimally at pH 6.0-7.0, 28-32 C and in the presence of 15-25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23T had MK-10 (H 4) and MK-9 (H4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C 17:0 (37.4 %), iso-C17:0 (14.8 %), iso-C15:0 (14.2 %), and iso-C16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23T (=DSM 45501T = CCUG 63368T = MTCC 11562T). © 2013 Springer Science+Business Media Dordrecht. Source


Meklat A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Meklat A.,Blida University | Bouras N.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Bouras N.,University of Ghardaia | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

A novel halophilic actinobacterium strai designated H8T was isolated from a Saharan soil sample collected in El-Golé South Algeria. Strain H8T was identified as representing a new genus using a polyphasic taxonomic approach. Phylogenetic analysis revealed that strain H8T shared the highest degree of 16S rRNA gene sequence similarity with ‘Mzabimyces algeriensis’ DSM 46680T (93.0 % Saccharopolysporaghardaiensis DSM 45606T (91.2 % Halopolyspora alba DSM 45976T (90.8 %) and Actinopolyspora mortivallis DSM 44261T (90.0 %). The strain was found to grow optimally at 28–35 ° at pH 6.0–7. and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was observed to be well developed and fragmented in liquid medium and on solid medium. The aerial mycelium was observed to be moderately abundant and to form long chains with non-motil smooth-surfaced and ovoid or spherical spores at maturity. The cell wall of strain H8T was found to contain meso-diaminopimelic acid. The whole-cell hydrolysates were found to mainly contain arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholin and MK-9(H4 MK-9(H2) and MK-10(H2) were found to be the predominant menaquinones. The major cellular fatty acids were determined to be anteiso-C17:0 and iso-C15:0. The genomic DNA G+C content of strain H8T was determined to be 71.3 mol%. The genotypic and phenotypic data showed that the strain represents a novel genus and specie for which the name Bounagaea algeriensis gen. nov sp. nov. is propose with the type strain H8T (=DSM 45966T = CECT 8470T). © 201 Springer International Publishing Switzerland. Source


Aouiche A.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Aouiche A.,University El Bachir El Ibrahimi | Bouras N.,Laboratoire Of Biologie Des Systemes Microbiens Lbsm | Bouras N.,University of Ghardaia | And 6 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2015

A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258T) to 97.8 % (Actinokineosporabaliensis NBRC 104211T). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H4). The major fatty acids were found to be iso-C16:0, iso-C15:0, iso-C16:1 H and iso-C16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineosporamzabensis sp. nov. is proposed, with the type strain PAL84T (=DSM 45961T = CECT 8578T). © 2014, Springer International Publishing Switzerland. Source

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