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Bennani B.,Laboratoire Of Biologie Des Cancers | Gilles S.,Laboratoire Of Transfert Doncologie Biologique Ap Hm | Fina F.,Laboratoire Of Transfert Doncologie Biologique Ap Hm | Nanni I.,Laboratoire Of Transfert Doncologie Biologique Ap Hm | And 9 more authors.
International Journal of Biological Markers

Background: The RAS/RAF/MEK/MAP kinase cascade transduces signals from the cell surface to the nucleus in order to control cellular responses including proliferation, differentiation and survival. We investigated the occurrence of BRAF exon 15 and KRAS codon 12 and 13 mutations in Moroccan patients with colorectal cancer. Methods: Sixty-two samples from patients with sporadic colorectal adenocarcinomas were studied for BRAF exon 15 and KRAS codon 12 and 13 mutations. DNA from paraffin-embedded tissue specimens was analyzed by a combination of polymerase chain reaction-high resolution melting and direct sequencing. Results: Of the analyzed specimens, 29% exhibited KRAS codon 12 or 13 mutations and only 1.6% carried a BRAF codon 600 mutation. KRAS mutations were more often observed in women (35.5%) than in men (22.6%). Patients in the age range between 41 and 60 years were more likely to be carriers of this mutation. No KRAS mutations were detected in patients aged >60 years. Conclusion: Despite the limited study sample, our data suggest that KRAS mutations arise more frequently than BRAF mutations in Moroccan patients with colorectal carcinomas. The KRAS mutation status must be assessed in a large cohort of Moroccan patients to confirm these findings and to determine whether this mutation in combination with extrinsic, environmental or microenvironmental factors might be involved in the high frequency of colorectal cancer in middle-aged Moroccans. © 2010 Wichtig Editore. Source

Bennani B.,Laboratoire Of Biologie Des Cancers | Bennani B.,University of Monastir | El Rhazi K.,Laboratoire Depidemiologie Et Of Sante Publique | Bennis S.,Laboratoire Of Biologie Des Cancers | And 5 more authors.
Journal Africain du Cancer

In Morocco, the cervical cancer remains a real public health problem where as its incidence decreases in the developed countries. This decrease is related to the early screening which bases on the Pap smear and human papillomavirus (HPV) test. The HPV is the causal agent of this disease; its prevalence varies according to the geographical regions. The objective of this study was to determine the HPV prevalence in three different regions in Morocco as well as related risk factors. The PCR "Polymerase chain reaction" was used for the HPV diagnosis. Results: Our results show that the infection rate differs between regions with alarming rates in the Fez city and in their regions. Two risk factors seem to be correlated to the HPV infection: the age of more than 45 years and the history of miscarriage or natural abortion. The rate of infection was more important at the woman's sexually active and who have never had children. Conclusion: A more particular intention must be lent to this category of women and a protocol of suitable screening taking into account specific risk factors in these regions must be established. © 2011 Springer Verlag France. Source

Bennani B.,University of Monastir | Bennani B.,Laboratoire Of Microbiologie | Bennis S.,Laboratoire Of Biologie Des Cancers | Nejjari C.,Laboratoire depidemiologie | And 7 more authors.
Journal of Infection in Developing Countries

Introduction: Epidemiological studies have shown the association between risk of developing cervical cancer and the persistence of high-risk papillomavirus types in addition to some co-factors. However, little is known about co-factors associated with human papillomavirus (HPV) infection, especially in developing countries. This study aims to determine HPV status and associated risk factors in women with normal cytology living in the north-central area of Morocco. Methodology: From February 2007 to December 2008, a total of 925 women consulting in the gynaecological department of Fez University Hospital were asked about sociodemographic characteristics and reproductive and sexual health. Cervical samples were collected for cytological examination and HPV DNA detection. Data collected from 751 women with normal cytology were used in this study to assess the correlation between HPV infection and potential risk factors. Results: High prevalence of HPV infection was detected (42.5%). The highest infection rate was observed in women aged >45 years and in those with history of abortion (OR:3.76; 95%CI[1.77-7.98]) fibroma, polyp or cysts (OR:1.68; 95%CI[1.07-2.65]). No significant association was detected with other reproductive health and risk factors including oral contraception. Conclusion: In spite of the insignificant association of HPV infection with age, health authorities should seriously consider and implement strategies to increase and maintain a cervical cancer screening programme in women aged 45 and above. More attention must be given to women with gynaecological history (abortion, fibroma, polyp or cysts) since these events may be predictors of HPV infection. Investigations on partner sexual behaviour and some specific hygienic habits, especially public Turkish bath use, are needed to clarify the HPV incidence in this region. © 2012 Bennani et al. Source

Boukhris S.A.,University Sidi Mohammed Ben Abdellah | Amarti A.,Service DAnatomie Pathologique CHU Hassan II | Amarti A.,Laboratoire Of Biologie Des Cancers | El Rhazi K.,Laboratoire Depidemiologie Et Of Recherche Clinique | And 11 more authors.

H. pylori persistent infection induces chronic gastritis and is associated with peptic ulcer disease and gastric carcinoma development. The severity of these diseases is related to human's genetic diversity, H. pylori genetic variability and environmental factors. To identify the prevalence of histo-pathological damages caused by H. pylori infection in Moroccan population, and to determine their association to H. pylori genotypes, a prospective study has been conducted during 3 years on patients attending the gastroenterology department of Hassan II University Hospital (CHU) of Fez, Morocco. A total of 801 Moroccan adults' patients were recruited; H. pylori was diagnosed and genotyped by PCR in biopsy specimens and histological exam was performed. We found a high rate of glandular atrophy. Chronic inflammation, neutrophil activity and glandular atrophy showed statistically significant association with H. pylori infection. However, intestinal metaplasia was inversely associated to this infection and no association was observed with gastric cancer cases. A statistically significant association was found between intestinal metaplasia and vacAs1 and vac Am1 genotypes in patients aged 50 years and more but not in younger. This last genotype is also associated to gastric cancer. In this study, gastric cancer showed no significant association with H. pylori. Further studies are warranted to determine the role of other etiological agents such as Epstein-Barr virus, human papillomavirus and possibly environmental and dietetic factors in the occurrence of this pathology. Copyright: © 2013 Alaoui Boukhris et al. Source

Boukhris S.A.,British Petroleum | Boukhris S.A.,University Sidi Mohammed Ben Abdellah | Benajah D.-A.,Service dHepato gastro enterologie | Benajah D.-A.,Laboratoire Of Biologie Des Cancers | And 12 more authors.
European Journal of Clinical Microbiology and Infectious Diseases

Helicobacter pylori infection is the etiologic agent of various gastric pathologies. The severity of disease outcome has been attributed to some H. pylori genotypes, which varies geographically. In Morocco, there are no data regarding the pattern of H. pylori genotypes; therefore, this is the first prospective study conducted in our country to investigate the genotype profiles (vacA and cagA) of H. pylori in patients with gastric pain. Endoscopic biopsies were obtained in patients attending the gastroenterology department of the Hospital University Hassan II of Fez for gastric pain and were directly used for H. pylori detection and genotyping by polymerase chain reaction (PCR). The SPSS software program was used to study the genotype correlation to different clinical outcomes. A total of 429 patients were included in this study, with an infection rate of 69.9%. cagA was detected in 42.3% of cases. However, vacA genotyping reveal a large predominance of s2m2. Infection with multiple strains was detected in 10.8% of cases and incomplete vacA was observed in 31.5%. In Morocco, vacA s1m1 was significantly associated to peptic ulcer diseases, while s2m2 was associated to gastritis. Moroccan H. pylori vacA genotype profiles differ from the Latin American, European, and South African profiles, with more similarities to the North African profile. Because of the small number of cases with gastric cancer, no correlations with H. pylori have been studied, so, further studies will be required in order to highlight the effects of those genes on this disease. © Springer-Verlag 2011. Source

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