Laboratoire Of Biochimie Toxicologie

Monastir, Tunisia

Laboratoire Of Biochimie Toxicologie

Monastir, Tunisia

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Bouzidi H.,Laboratoire Of Biochimie | Hayek D.,Laboratoire Of Biochimie | Nasr D.,Laboratoire Of Biochimie | Daudon M.,Groupe Hospitalier Necker Enfants Malades | Najjar M.F.,Laboratoire Of Biochimie Toxicologie
Annales de Biologie Clinique | Year: 2011

Renal tubular acidosis (RTA) is a tubulopathy characterized by metabolic acidosis with normal anion gap secondary to abnormalities of renal acidification. RTA can be classified into four main subtypes: distal RTA, proximal RTA, combined proximal and distal RTA, and hyperkalemic RTA. Distal RTA (type 1) is caused by the defect of H + secretion in the distal tubules and is characterized by the inability to acidify the urine below pH 5.5 during systemic acidemia. Proximal RTA (type 2) is caused by an impairment of bicarbonate reabsorption in the proximal tubules and characterized by a decreased renal bicarbonate threshold. Combined proximal and distal RTA (type 3) secondary to a reduction in tubular reclamation of bicarbonate and an inability to acidify the urine in the face of severe acidemia. Hyperkalemic RTA (type 4) may occur as a result of aldosterone deficiency or tubular insensitivity to aldosterone. Clinicians should be alert to the presence of RTA in patients with an unexplained normal anion gap acidosis, hypokalemia, recurrent nephrolithiasis and nephrocalcinosis. The mainstay of treatment of RTA remains alkali replacement.


Zohoun A.G.C.,Laboratoire Of Biochimie Toxicologie | Hounmenou K.,Laboratoire Of Biochimie Toxicologie | Mechtani S.E.I.,Laboratoire Of Biochimie Toxicologie | Razine R.,Laboratoire Of Biostatistique | And 4 more authors.
Annales de Biologie Clinique | Year: 2013

Measurement of hCG remains today central for diagnosis, treatment and follow-up of gestational trophoblastic diseases (GTD). In order to evaluate this contribution, we conducted a prospective cohort study in the Service of highrisk pregnancy of Rabat Maternity Les Orangers and the Laboratory of Rabat Military Teaching Hospital Mohammed V over a period of eighteen months. 35 patients were included. The hCG assay was determined by electrochemiluminescence. The general frequency of the GTD is of 0.33/100 childbirth. The average age of our patients was 30.5 years. 26 patients had hCG level abnormal and higher than 200 000 UI/mL when diagnosed with GTD; 34 patients had simple endo-uterine aspiration and 1 a chemotherapy. Among 34 patients, 25 (73.5%) had a favourable evolution characterized by normal hCG level within 3 to 13 weeks and complete remission. Serum hCG remained stable and negative in all these patients with a follow-up of 18 months. 9 patients had unfavourable evolution characterized by reaxent after negativation or stagnation of hCG levels. A rigorous monitoring of hCG levels during treatment and follow-up is essential to improve forecast of these diseases. © Copyright 2007 John Libbey Eurotext - All rights reserved.


PubMed | Laboratoire Of Biochimie Toxicologie, Service de gynecologie obstetrique, Laboratoire Of Biostatistique and Maternite University Les Orangers
Type: Evaluation Studies | Journal: Annales de biologie clinique | Year: 2013

Measurement of hCG remains today central for diagnosis, treatment and follow-up of gestational trophoblastic diseases (GTD). In order to evaluate this contribution, we conducted a prospective cohort study in the Service of high-risk pregnancy of Rabat Maternity Les Orangers and the Laboratory of Rabat Military Teaching Hospital Mohammed V over a period of eighteen months. 35 patients were included. The hCG assay was determined by electrochemiluminescence. The general frequency of the GTD is of 0.33/100 childbirth. The average age of our patients was 30.5 years. 26 patients had hCG level abnormal and higher than 200 000 UI/mL when diagnosed with GTD; 34 patients had simple endo-uterine aspiration and 1 a chemotherapy. Among 34 patients, 25 (73.5%) had a favourable evolution characterized by normal hCG level within 3 to 13 weeks and complete remission. Serum hCG remained stable and negative in all these patients with a follow-up of 18 months. 9 patients had unfavourable evolution characterized by reaxent after negativation or stagnation of hCG levels. A rigorous monitoring of hCG levels during treatment and follow-up is essential to improve forecast of these diseases.


Bouzidi H.,Laboratoire Of Biologie Medicale | Majdoub A.,University of Monastir | Daudon M.,Service dexploration fonctionnelle | Najjar M.F.,Laboratoire Of Biochimie Toxicologie
Nephrologie et Therapeutique | Year: 2016

Primary hyperoxalurias (PH) are inborn errors in the metabolism of glyoxalate and oxalate with recessive autosomal transmission. As a result, an increased endogenous production of oxalate leads to exessive urinary oxalate excretion. PH type 1, the most common form, is due to a deficiency of the peroxisomal enzyme alanine: Glyoxylate aminotransferase (AGT) in the liver. PH type 2 is due to the deficiency of the glyoxylate reductase/hydroxypyruvate réductase, present in the cytosol of hepatocytes and leucocytes. PH type 3 is linked to the gene HOGA1, encoding a mitochondrial enzyme, the 4-hydroxy-2-oxo-glutarate aldolase. Recurrent urolithiaisis and nephrocalcinosis are the markers of the disease. As a result, a progressive dysfunction of the kidneys is commonly observed. At the stage of severe chronic kidney disease, plasma oxalate increase leads to a systemic oxalosis. Diagnostic is often delayed and it based on stone analysis, cristalluria, oxaluria determination and DNA analysis. Early initiation of conservative treatment including high fluid intake and long-term co-administration of inhibitors of calcium oxalate crystallization and pyridoxine, could efficiently prevent end stage renal disease. In end stage renal failure, a combined liver-kidney transplantation corrects the enzyme defect. © 2016 Association Société de néphrologie.


Hellara I.,Laboratoire Of Biochimie Toxicologie | Fekih O.,Laboratoire Of Biochimie Toxicologie | Triki S.,Laboratoire Of Biochimie Toxicologie | Elmay A.,Laboratoire Of Biochimie Toxicologie | And 2 more authors.
Annales de Biologie Clinique | Year: 2014

Capillary electrophoresis of serum proteins is a fast, reliable and simple technique, but many interference exist. The objective of our work is to study the interference of bilirubin on this technique; 70 icteric sera were analysed on Capillarys TM (Sebia). A second electrophoresis was performed on 40 samples after bilirubin photodegradation. The bilirubin and serum proteins were determinated respectively by Jendrassik and Grof and biuret methods on Konélab 20i™ (Thermo Electron Corporation).We found abnormal spreading of the albumin fraction of the anode side wich constitute sometimes an isolated fraction in the traditional area of pre-albumin migration. This fraction varies from 2.0 ± 2.0% (0.0 to 7.3%) or 0.98 ± 1.53 g/L (0 to 5.3 g/L) and it seems to be related to the direct bilirubin since, following overloading sera with a solution of bilirubin, no further fraction was recovered. An average decrease of bilirubin after photodegradation of 58 ± 17% (26-89%) is followed by a decrease in the same order 64 ± 38% (10-100%) of the additional fraction. Acetate cellulose electrophoresis of the same samples showed no variation. The high bilirubin levels seem modify slightly the electrophoretic profile. However the impact.


Teyeb H.,Institute Des Regions Arides Of Medenine | Teyeb H.,Laboratoire Of Biochimie Toxicologie | Zouari S.,Institute Des Regions Arides Of Medenine | Douki W.,Laboratoire Of Biochimie Toxicologie | And 2 more authors.
Acta Horticulturae | Year: 2010

Astragalus species are medicinal and toxic plants, and many of them have been studied before. Astragalus gombiformis Pomel is a spontaneous desertic plant of Tunisian flora. This plant is of moderate appetability, probably in view of the presence of many volatile compounds toxic for livestock. In this study, we extract and analyze, for the first time, the essential oil of this plant. The oils are extracted by hydrodistillation from different organs and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) using a capillary colonne HP-5MS. The essential oil composition seems to be complex and varied between flowering and fructification stages. The main compounds are: 6,10,14-triméthylpentadecan-2-one, hexadecanoic acid, phytol and dillapiole. Many volatiles of interest are identified such as butylated hydroxytoluene (BHT), an antioxidant compound. Other compounds which can be responsible for toxicity are identified with different amounts between organs, such as caprolactam and benzeneacetonitrile.


PubMed | Laboratoire Of Biochimie Toxicologie
Type: Journal Article | Journal: Annales de biologie clinique | Year: 2014

Capillary electrophoresis of serum proteins is a fast, reliable and simple technique, but many interference exist. The objective of our work is to study the interference of bilirubin on this technique; 70 icteric sera were analysed on Capillarys (Sebia). A second electrophoresis was performed on 40 samples after bilirubin photodegradation. The bilirubin and serum proteins were determinated respectively by Jendrassik and Grof and biuret methods on Konlab 20i (Thermo Electron Corporation). We found abnormal spreading of the albumin fraction of the anode side wich constitute sometimes an isolated fraction in the traditional area of pre-albumin migration. This fraction varies from 2.0 2.0% (0.0 to 7.3%) or 0.98 1.53 g/L (0 to 5.3 g/L) and it seems to be related to the direct bilirubin since, following overloading sera with a solution of bilirubin, no further fraction was recovered. An average decrease of bilirubin after photodegradation of 58 17% (26-89%) is followed by a decrease in the same order 64 38% (10-100%) of the additional fraction. Acetate cellulose electrophoresis of the same samples showed no variation. The high bilirubin levels seem modify slightly the electrophoretic profile. However the impact of the interference on the interpretation of electrophoretic trace is negligible.


PubMed | Laboratoire Of Biochimie Toxicologie
Type: Journal Article | Journal: La Tunisie medicale | Year: 2012

The clinical and biological characteristics of urolithiasis in children are still evolving in our country. Since the eighties no study has been conducted to determine incidence of urolithiasis in Tunisia.To define the current status of urolithiasis in Tunisian children.The records of 414 children with urolithiasis treated between 1983 and 2007 were found in a multi-centric study which took into consideration to age, sex, and history diagnosis and physical, laboratory, and radiologic findings. Clinical and biological date were performed in 344 cases. Stone analysis was performed by spectrophotometry.The incidence of paediatric urolithiasis has steadily decreased from 0.7/ 10 000 of the child population / year in 1991-1994 to 0.1 cases/10 000 children/ year in 2007. There was a continuous decrease in bladder stone over the past 25 years (47.1% in 1982-1986 Vs 10.2% in 2002-2007). Stones were homogeneous in 31.4% of cases. The main component was calcium oxalate stones in 36.4% of the cases. Since the eighties the increase of oxalates stones (15.6% in eighties Vs 51.5% actually) were with depends to purines and struvite stones.Epidemiologic characteristics of the urinary lithiasis (sex, age, localization and composition) have changed in Tunisia, due to the the improvements of standard of living. The occidentalization of the nutritional practices and the receding of the culinary traditions gave birth to new risks of urolithiasis characterized by the emergence of calcium oxalate stones.

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