Laboratoire National Of La Protection Des Vegetaux

Angers, France

Laboratoire National Of La Protection Des Vegetaux

Angers, France
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Monger W.,UK Environment Agency | Tomlinson J.,UK Environment Agency | Booonham N.,UK Environment Agency | Marn M.V.,Agricultural Institute of Slovenia | And 10 more authors.
Journal of Virological Methods | Year: 2010

Assays based on real-time PCR (TaqMan®) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd. © 2010.

Robin C.,French National Institute for Agricultural Research | Piou D.,French National Institute for Agricultural Research | Feau N.,French National Institute for Agricultural Research | Douzon G.,DRAAF Center | And 2 more authors.
Forest Pathology | Year: 2011

Phytophthora lateralis has been isolated from root and collar lesions in Port-Orford Cedar (POC) trees (Chamaecyparis lawsoniana) in north-western France (Brittany). These trees, planted in hedgerows, displayed symptoms similar to the typical symptoms of POC root disease. Until now, the disease has been found outside of the nurseries only in western North America. Aerial symptoms, not associated with root or collar infections, were also observed, and P. lateralis was isolated from branch lesions. Similar symptoms were previously observed only in one POC root disease site, located in the Pacific coast of Oregon where climatic conditions are similar to those occurring in Brittany. The reported aetiology as well as the morphological characteristics (deciduous sporangia) of P. lateralis suggests that this species could be air-dispersed, as described for P. ramorum, a closely related species. This outbreak of P. lateralis in Brittany in farming landscapes associated with the aerial spread of this pathogen represents a new threat for European countries. © 2010 Blackwell Verlag GmbH.

Pellizzari G.,University of Padua | Germain J.-F.,Laboratoire National Of La Protection Des Vegetaux
Zootaxa | Year: 2010

The adult female, 1st-instar nymph, 2nd-instar male and 2nd-instar female nymphs of a new species of felt scale, Acanthococcus mariannae Pellizzari, are described and illustrated. The new species was collected off Leptospermum scoparium (Myrtaceae) in Italy and France. An identification key of Eriococcid species known to occur on Leptospermum spp is also provided. Copyright © 2010 Magnolia Press.

Olivier V.,Laboratoire National Of La Protection Des Vegetaux | Baloche A.,Laboratoire National Of La Protection Des Vegetaux | Drouin A.,Laboratoire National Of La Protection Des Vegetaux | Audusseau C.,Laboratoire National Of La Protection Des Vegetaux | And 2 more authors.
EPPO Bulletin | Year: 2010

Bacterial canker of tomato is a disease caused by Clavibacter michiganensis subsp. michiganensis, a quarantine bacterium, the spread of which has not been completely controlled in spite of the phytosanitary measures taken within the EPPO region. Since 2008 the French National Laboratory for Plant Health (LNPV) has been working on the assessment of the methods used in laboratories to detect the presence of Clavibacter michiganensis subsp. michiganensis in tomato seeds i.e. dilution plating on semi-selective media and immunofluorescence. In the 1st stage of the assessment, a methods comparison study was performed with reference strains to determine the performance criteria of the tests in optimal conditions. In the 2nd stage, an inter-laboratory study on naturally and artificially contaminated seeds was performed with 8 laboratories from 6 European countries. This study demonstrated the strengths and weaknesses of the tests currently in use. Two laboratories took the opportunity the collaborative study offered to evaluate alternative tests: BIO-PCR and IMS-plating. These could offer interesting alternatives to optimise the detection procedure for Clavibacter michiganensis subsp. michiganensis on tomato seeds. © 2010 The Authors. Journal compilation © 2010 OEPP/EPPO.

Ioos R.,Laboratoire National Of La Protection Des Vegetaux | Fourrier C.,Laboratoire National Of La Protection Des Vegetaux
EPPO Bulletin | Year: 2011

This work reports the optimization and provides statistically supported validation data for a real-time detection tool targeting the emerging fungus Chalara fraxinea. The test proved to be specific and inclusive, based on computer simulation and in vitro assessments, and could detect as little as 20fg of C.fraxinea DNA. The duplex real-time polymerase chain reaction assay proved to be significantly more efficient and rapid than isolation on agar plates. To monitor the performance of the whole analysis process and to ensure reliability of the results, a set of controls was used systematically during the assays. A diagnosis flow diagram is proposed. The Laboratoire National de la Protection des végétaux was recently accredited by the French accreditation body for this protocol. © 2011 The Authors. Journal compilation © 2011 OEPP/EPPO.

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