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Pradel N.,Aix - Marseille University | Pradel N.,Laboratoire Mixte International Contaminants et Ecosystemes Marins Sud Mediterraneens LMI COSYS Med | Fuduche M.,Aix - Marseille University | Ollivier B.,Aix - Marseille University | Ollivier B.,Laboratoire Mixte International Contaminants et Ecosystemes Marins Sud Mediterraneens LMI COSYS Med
Environmental Science and Pollution Research | Year: 2016

In this study, we report for the first time the presence of magnetotactic bacteria (MTB) on the Northeastern Atlantic coast. Microscopy observations indicated a heterogeneous population of MTB morphotypes. The analysis of the 16S rDNA by pyrosequencing technology revealed four operational taxonomic sequence units affiliated within the Magnetococcales order, class Alphaproteobacteria. One of them was closely related to sequences of MTB from the Tunisian coast, central Mediterranean Sea. This work offers information on anew environmental context and on biogeography of MTB, highlights the putative impact that marine currents may have on MTB distribution on Earth, and underlines the role that pristine or polluted areas may play on the structure of the MTB communites. © 2015, Springer-Verlag Berlin Heidelberg. Source


Pradel N.,Aix - Marseille University | Pradel N.,Laboratoire Mixte International Contaminants et Ecosystemes Marins Sud Mediterraneens LMI COSYS Med | Cayol J.-L.,Aix - Marseille University | Fardeau M.-L.,Aix - Marseille University | And 5 more authors.
Environmental Science and Pollution Research | Year: 2016

The occurrence of magnetotactic bacteria (MTB) on a Tunisian marine coast exposed to heavy metals pollution (Sfax, Gulf of Gabès, Mediterranean Sea) was investigated. The MTB population of this Southern Mediterranean coast was compared to the MTB populations previously investigated on the French Northern Mediterranean coast. A dominant MTB coccus morphotype was observed by microscopy analysis. By pyrosequencing technology, the analysis of the 16S ribosomal RNA (rDNA) revealed as much as 33 operational taxonomic sequence units (OTUs) close to sequences of MTB accessible in the databases. The majority were close to MTB sequences of the “Med group” of α-Proteobacteria. Among them, a dominant OTU_001 (99 % of the MTB sequences) affiliated within the Magnetococcales order was highlighted. Investigating the capacities of this novel bacterium to be used in bioremediation and/or depollution processes could be envisaged. © 2015, Springer-Verlag Berlin Heidelberg. Source


Abdallah M.B.,University of Sfax | Abdallah M.B.,Aix - Marseille University | Karray F.,University of Sfax | Karray F.,Laboratoire Mixte International Contaminants et Ecosystemes Marins Sud Mediterraneens LMI COSYS Med | And 12 more authors.
Extremophiles | Year: 2016

Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 × 106 DNA copies g−1 whereas archaea varied between 5 × 105 and 106 DNA copies g−1 in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid. © 2015, Springer Japan. Source

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