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Sbissi I.,Laboratoire Microorganismes and Biomolecules Actives | Sbissi I.,Institute des Regions Arides | Neffati M.,Institute des Regions Arides | Boudabous A.,Laboratoire Microorganismes and Biomolecules Actives | And 2 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2010

The molecular phylogeny and comparative morphological studies reported here provide evidence for the recognition of the genus Picoa, an hypogeous desert truffle, in the family Pyronemataceae (Ascomycota, Pezizales). Picoa juniperi and Picoa lefebvrei were reassigned to the genus Picoa based on large subunit (LSU) sequence (28S) rDNA and internal transcribed spacer (ITS) rDNA (including the partial 18S, ITS1, ITS2, 5.8S gene, and partial 28S of the nuclear rDNA) data. Morphological studies of spores, asci, perida, and gleba revealed high similarities between P. lefebvrei and P. juniperi, thereby confirming the membership of both species in the genus Picoa. These two species were primarily distinguishable based on ascospore ornamentation. © 2010 Springer Science+Business Media B.V.


Essoussi I.,Laboratoire Microorganismes and Biomolecules Actives | Boujmil R.,Laboratoire Microorganismes and Biomolecules Actives | Nouioui I.,Laboratoire Microorganismes and Biomolecules Actives | Abbassi-Ghozzi I.,Laboratoire Microorganismes and Biomolecules Actives | And 3 more authors.
Geomicrobiology Journal | Year: 2012

Stone surfaces in Sahara desert are continuously exposed to higher solar radiation, temperature, aridness and lack of nutrients. With the exception of members of Geodermatophilaceae, actinobacteria inhabiting such hard environments have attracted less interest. Here we addressed the phylogenetic diversity of the culturable Actinobacteria from Sahara desert stones and monuments. Amplified rDNA restriction analysis of the isolates (ARDRA) followed by 16S rRNA gene sequencing permitted us to delineate 25 ARDRA types that have been assigned to 6 actinobacterial genera; Arthrobacter, Micromonospora, Actinoplanes, Kineococcus and Nocardia. The isolates have been typed on the basis of a major group of hydrolytic activities: esterase. The particularly rich pool of esterase patterns previously recorded for members of Geodermatophilaceae was extended to other sympatric prevalent actinobacteria on stone surfaces that belong mainly to the genera Actinoplanes and Kineococcus. The results of the present study were discussed in view of ecological significance of these actinobacteria in stone materials. © 2012 Copyright Taylor and Francis Group, LLC.

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