Aarnink A.,Laboratoire dImmunogenetique Moleculaire LIMT |
Jacquelin B.,Institute Pasteur Paris |
Dauba A.,Laboratoire dImmunogenetique Moleculaire LIMT |
Hebrard S.,Laboratoire dImmunogenetique Moleculaire LIMT |
And 3 more authors.
African green monkeys (AGM) are among themost widely used nonhuman primate models used in various fields of medical research. One species of AGMthat originated from West Africa, Chlorocebus sabaeus, was introduced three centuries ago in the Caribbean islands.We present here a systematic study of the major histocompatibility complex (MHC) polymorphism of Caribbean AGM which is currently frequently used as an animal model. We studied 54 animals originated from Barbados (N=25) or Saint Kitts (N=29). The MHC polymorphism was characterized by means of 17 MHC microsatellites spread across MHC and DRB genotyping by DGGE sequencing. We defined nine frequent MHC haplotypes of which two were found in the two insular populations suggesting either past exchanges between the two populations or a common origin of the founders of the two populations. By the analysis of a previously described EST library, we characterized 38 MHC cDNA sequences (17 class I and 21 class II). In conclusion, we characterized for the first time the MHC polymorphism of Barbados and Saint Kitts AGM.We found a restricted polymorphism due to a founding effect, which is responsible for a strong bottleneck. The poorness of MHC polymorphism observed in the Caribbean AGM populations is similar to that observed in the Mauritian cynomolgus macaque population. © 2014 Springer-Verlag Berlin Heidelberg. Source