Laboratoire Dhydrologie Of Nancy

Vandœuvre-lès-Nancy, France

Laboratoire Dhydrologie Of Nancy

Vandœuvre-lès-Nancy, France
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Perrat E.,CNRS Interdisciplinary Laboratory for Continental Environments | Parant M.,CNRS Interdisciplinary Laboratory for Continental Environments | Py J.-S.,Laboratoire Dhydrologie Of Nancy | Rosin C.,Laboratoire Dhydrologie Of Nancy | Cossu-Leguille C.,CNRS Interdisciplinary Laboratory for Continental Environments
Environmental Science and Pollution Research | Year: 2017

In this study, the presence of anthropogenic gadolinium (Gd) was evaluated in rivers, close to wastewater treatment plant outputs. Then, one site was selected for in situ experiments to assess the bioaccumulation of Gd in the digestive gland and in the gills of two bivalves (Dreissena rostriformis bugensis and Corbicula fluminea). For both organisms, the results suggested that the bioaccumulation of Gd can be observed when organisms are exposed in a geogenic and anthropogenic Gd mixture. In order to observe if Gd can bioaccumulate in tissues of bivalves when the ion is only present as the main anthropogenic speciation of Gd, i.e., Gd-contrast agents (Gd-CAs), the gadoteric acid was used for a laboratory experiment. In this case, the presence of Gd was clearly detected in a significant amount in the digestive glands of D. rostriformis bugensis and C. fluminea while low concentrations are measured in the gills. For the first time, these results clearly showed that Gd can bioaccumulate in bivalve tissues even when it is only present as Gd-CAs. Biochemical activities were measured in the digestive gland and in the gills of the bivalves to assess the effects of Gd-CA bioaccumulation. No significant variations were observed in the gills. Concerning the digestive gland, after 7 days of exposure at 10 μg L−1 of Gd as Gd-CA speciation, GST activity in D. rostriformis bugensis and lipid hydroperoxide and mitochondrial electron transfer system in C. fluminea had increased. The results suggest an acclimation of the organisms to the presence of Gd-CAs in the medium within less than 21 days. © 2017 Springer-Verlag Berlin Heidelberg

Pasquini L.,CNRS Interdisciplinary Laboratory for Continental Environments | Munoz J.-F.,Laboratoire Dhydrologie Of Nancy | Pons M.-N.,University of Lorraine | Yvon J.,CNRS Interdisciplinary Laboratory for Continental Environments | And 5 more authors.
Science of the Total Environment | Year: 2014

The occurrence in urban wastewater of eight micropollutants (erythromycin, ibuprofen, 4-nonylphenol (4-NP), ofloxacin, sucralose, triclosan, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS)) originating from household activities and their fate in a biological wastewater treatment plant (WWTP) were investigated. Their concentrations were assessed in the liquid and solid phases (sewage particulate matter and wasted activated sludge (WAS)) by liquid chromatography-tandem mass spectrometry.The analysis of sewage from two different urban catchments connected to the WWTP showed a specific use of ofloxacin in the mixed catchment due to the presence of a hospital, and higher concentrations of sucralose in the residential area.The WWTP process removed over 90% of ibuprofen and triclosan from wastewater, while only 25% of ofloxacin was eliminated. Erythromycin, sucralose and PFOA were not removed from wastewater, the influent and effluent concentrations remaining at about 0.7. μg/L, 3. μg/L and 10. ng/L respectively. The behavior of PFOS and 4-nonylphenol was singular, as concentrations were higher at the WWTP outlet than at its inlet. This was probably related to the degradation of some of their precursors (such as alkylphenol ethoxylates and polyfluorinated compounds resulting in 4-NP and PFOS, respectively) during biological treatment.4-NP, ofloxacin, triclosan and perfluorinated compounds were found adsorbed on WAS (from 5. ng/kg for PFOA to 1.0. mg/kg for triclosan).The statistical methods (principal component analysis and multiple linear regressions) were applied to examine relationships among the concentrations of micropollutants and macropollutants (COD, ammonium, turbidity) entering and leaving the WWTP. A strong relationship with ammonium indicated that some micropollutants enter wastewater via human urine. A statistical analysis of WWTP operation gave a model for estimating micropollutant output from the WWTP based on a measurement of macropollution parameters. © 2014 Elsevier B.V.

Garnier A.,Laboratoire Dhydrologie Of Nancy | Rosin C.,Laboratoire Dhydrologie Of Nancy | Jedor B.,Ministere Charge de la Sante | Dauchy X.,Laboratoire Dhydrologie Of Nancy | And 2 more authors.
Journal Europeen d'Hydrologie | Year: 2012

Perchlorate in drinking water is a new concern for public and sanitary authority due to the recent detection in drinking water and its effects on the thyroid function. Health Ministry entrusted a national survey of perchlorate in drinking water to French Agency for Food, Environmental and Occupational Health & Safety (Anses) to have data on exposure by drinking water. In association with Regional Agencies of Health and the Ministry of Health, the Nancy laboratory for Hydrology of organized on all the metropolitan and overseas departments a sampling representing with 25 % of the consumer population. Furthermore, a campaign of analyses on vulnerable sites was organized in association with FP2E in 34 departments of metropolitan France. Perchlorate was measured with a limit of quantification of 0.5 μg.L-1 thanks to an analysis by two dimensions ion chromatography coupled with a mass spectrometer The results highlight for the national campaign: - For the resources: contents lower than 4 μg.L-1 in 94 % of the cases and lower than 15 μg.L-1 in 99 % of the cases ; - For treated waters: contents lower than 4 μg.L-1 in 97 % of the cases and lower than 15 μg.L-1 in 100 % of the cases. Samples taken in vulnerable zone in association with the Professional Federation of the Companies of the Water highlighted slightly superior concentration levels but systematically lower than 15 μg.L-1. The contamination of drinking water by perchlorate is very localized and concern mainly the regions of the North, Picardy on sites coinciding with zones of historic bombardment. © ASEES, 2013.

Bach C.,Laboratoire Dhydrologie Of Nancy | Bach C.,CNRS Jean Lamour Institute | Dauchy X.,Laboratoire Dhydrologie Of Nancy | David L.,Laboratoire des Materiaux Polymeres et Biomateriaux LMPB | Itienne S.,CNRS Jean Lamour Institute
Materiaux et Techniques | Year: 2011

Several studies have shown the presence of compounds not expected a priori in bottled water. One of the possible sources is the diffusion of constituents from PET E to water (monomers, catalysts, additives or degradation products) after exposure to different conditions of storage, time, temperature or/and sunlight. The migration of acetaldehyde and antimony has been observed at room temperature and at 40 °C for 45 days. At the same time, a physico-chemical characterisation of the PET E bottle has been performed. IRTF spectroscopy was carried out in order to determine the nature of polymeric material and its structural state. The crystallinity ratio and the glass transition temperature were observed by calorimetric measurements. Low frequency mechanical spectroscopy was used to characterise the dynamics of molecular motions in the polymer. Finally, X-ray scattering (WAXS and SAXS) was achieved to determine the structure and arrangement of PET E chains in the bottle. © EDP Sciences, 2011.

Pasquini L.,University of Lorraine | Merlin C.,CNRS Laboratory of Physical Chemistry and Microbiology for the Environment | Hassenboehler L.,University of Lorraine | Munoz J.-F.,Laboratoire Dhydrologie Of Nancy | And 2 more authors.
Science of the Total Environment | Year: 2013

The impact of eight household micropollutants (erythromycin, ofloxacin, ibuprofen, 4-nonylphenol, triclosan, sucralose, PFOA and PFOS (PFAAs)) on the laboratory bacterial strain Escherichia coli MG1655 and on activated sludge from an urban wastewater treatment plant was studied.Growth-based toxicity tests on E. coli were performed for each micropollutants. The effect of micropollutants on activated sludge (at concentrations usually measured in wastewater up to concentrations disturbing the bacterial growth of E. coli) was examined in batch reactors and by comparison to a control reactor (without micropollutants). The bound extracellular polymeric substances (EPS) secreted by the sludge were measured by size exclusion chromatography and their overexpression was considered as an indicator of bacteria sensitivity to environmental changes. The chemical oxygen demand (COD) and the ammonium concentration were monitored to evaluate the biomass ability to remove the macropollution.Some micropollutants induced an increase of bound EPS in activated sludge flocs at concentrations depending on the micropollutant: erythromycin from 100μg/L, ofloxacin from 10μg/L, triclosan from 0.5μg/L, 4-nonylphenol from 5000μg/L and PFAAs from 0.1μg/L. This suggests that the biomass had to cope with new conditions. Moreover, at high concentrations of erythromycin (10. mg/L) and ibuprofen (5. mg/L) bacterial populations were no longer able to carry out the removal of macropollution.Ibuprofen induced a decrease of bound EPS at all the studied concentrations, probably reflecting a decrease of general bacterial activity. The biomass was not sensitive to sucralose in terms of EPS production, however at very high concentration (1. g/L) it inhibited the COD decrease.Micropollution removal was also assessed. Ibuprofen, erythromycin, ofloxacin, 4-nonylphenol and triclosan were removed from wastewater, mainly by biodegradation. Sucralose and PFOA were not removed from wastewater at all, and PFOS was slightly eliminated by adsorption on sludge. © 2013 .

Madec J.Y.,Unite Antibioresistance et Virulence Bacteriennes | Haenni M.,Unite Antibioresistance et Virulence Bacteriennes | Ponsin C.,Unite Antibioresistance et Virulence Bacteriennes | Kieffer N.,Unite Antibioresistance et Virulence Bacteriennes | And 2 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2016

Drinking water has rarely been recognized as a source of antimicrobial resistance for humans, and only in low-income countries. Here, a sequence type 48 Escherichia coli isolate carrying the blaCTX-M-1 IncI1/ST3 plasmid was recovered from drinking water in France. This plasmid was similar to other blaCTX-M-1 IncI1/ST3 plasmids found previously in animals and humans. Our findings highlight the possible human transfer of extended-spectrum β-lactamase (ESBL) genes through drinking water in high-income countries. © 2016, American Society for Microbiology. All Rights Reserved.

Tissier A.,Laboratoire Dhydrologie Of Nancy | Tissier A.,French Institute of Health and Medical Research | Denis M.,Laboratoire Of Ploufragan Plouzane | Hartemann P.,French Institute of Health and Medical Research | Gassilloud B.,Laboratoire Dhydrologie Of Nancy
Applied and Environmental Microbiology | Year: 2012

Investigations of Campylobacter jejuni and Campylobacter coli in samples of drinking water suspected of being at the origin of an outbreak very often lead to negative results. One of the reasons for this failure is the small volume of water typically used for detecting these pathogens (10 to 1,000 ml). The efficiencies of three microfilters and different elution procedures were determined using real-time quantitative PCR to propose a procedure allowing detection of Campylobacter in 20 liters of drinking water or low-turbidity water samples. The results showed that more than 80% of the bacteria inoculated in 1 liter of drinking water were retained on each microfilter. An elution with a solution containing 3% beef extract, 0.05Mglycine at pH 9, combined with direct extraction of the bacterial genomes retained on the cellulose ester microfilter, allowed recovery of 87.3% (±22% [standard deviation]) of Campylobacter per 1 liter of tap water. Recoveries obtained from 20-liter volumes of tap water spiked with a C. coli strain were 69.5% (±10.3%) and 78.5% (±15.1%) for 91 CFU and 36 CFU, respectively. Finally, tests performed on eight samples of 20 liters of groundwater collected from an alluvial well used for the production of drinking water revealed the presence of C. jejuni and C. coli genomes, whereas no bacteria were detected with the normative culture method in volumes ranging from 10 to 1,000 ml. In the absence of available epidemiological data and information on bacterial viability, these last results indicate only that the water resource is not protected from contamination by Campylobacter. © 2012, American Society for Microbiology.

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