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Le Touquet – Paris-Plage, France

Dumont M.,Laboratoire dEylau Unilabs | Junca A.-M.,Laboratoire dEylau Unilabs | Belloc S.,Laboratoire dEylau Unilabs | Cohen-Bacrie P.,Laboratoire dEylau Unilabs | And 5 more authors.
Andrologie | Year: 2011

Introduction: MSOME (Motile Sperm Organellar Morphology Examination) is a new method for real-time evaluation of sperm morphology under 6600x high magnification. ICSI modified procedure with sperm selected by MSOME is named IMSI (Intracytoplasmic Morphologically Selected sperm Injection). IMSI has been developed to improve ongoing pregnancy rate in couples with repeated implantation failure. Material and methods: The study concern an observational cohort of 11535 ICSI performed with fresh ejaculated sperm in our ART lab between January 2004 and July 2009. Among them, 2509 were realized with IMSI. The primary outcome measures were cleavage rate per injected oocyte on day 2, clinical pregnancy and abortion rates. Comparisons were performed using Chi square2 test and univariate analysis of variance. Results: There were no significant difference between conventional ICSI and IMSI groups in term of cleavage and pregnancy rates. Couples with abnormal sperm (teratozoospermia, oligozoospermia and oligoteratozoospermia) and no previous ICSI failure, had a significantly higher clinical pregnancy with IMSI than with ICSI (34. 4% vs. 27. 1%, p = 0. 02). Furthermore, pregnancies obtained in patients with teratozoospermia were associated with a lower abortion rate after IMSI than after ICSI, close to significance (12. 6% vs. 19. 6%, p = 0. 08). Conclusion: In cases of severe teratozoospermia, IMSI appears to improve pregnancy rate and pregnancy outcome. © 2011 Springer. Source


Belloc S.,Laboratoire dEylau Unilabs | Benkhalifa M.,Laboratoire dEylau Unilabs | Cohen-Bacrie M.,Laboratoire dEylau Unilabs | Dalleac A.,Laboratoire dEylau Unilabs | And 2 more authors.
Fertility and Sterility | Year: 2014

Objective To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. Design Retrospective cohort study. Setting Clinical andrology laboratory. Patient(s) A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazoospermic men presenting for infertility evaluation. Intervention(s) None. Main Outcome Measure(s) Clinical parameters, conventional semen parameters, and sperm DNA fragmentation assessed by flow cytometry-based TUNEL assay and reported as percent sperm DNA fragmentation (%SDF). Result(s) The mean (±SD) %SDF and the proportion of men with high %SDF (>30%) were significantly lower in the normozoospermic compared with the entire cohort of 4,345 evaluable infertile men (17.6% ± 10.1% vs. 20.7% ± 12.4% and 11% vs. 20%, respectively). In the group of 1,974 normozoospermic men, %SDF was positively correlated with paternal age (r = 0.17) and inversely correlated with progressive motility (r = -0.26). In the subset of normozoospermic men with sperm parameters above the 50th percentile (≥73 × 106 sperm/mL, ≥55% progressive motility, and ≥14% normal forms, World Health Organization 2010 guidelines), 5% (4 of 83) had elevated %SDF (>30%). Conclusion(s) In this large cohort of normozoospermic men presenting for infertility evaluation, DNA fragmentation level is related to sperm motility and paternal age, and 11% of these men have high levels of sperm DNA fragmentation. Furthermore, the data indicate that a nonnegligible proportion (5%) of normozoospermic men with high-normal sperm parameters may also have significant sperm DNA fragmentation. Copyright © 2014 American Society for Reproductive Medicine, Published by Elsevier Inc. Source


Montjean D.,Service de Medicine et Biologie de la Reproduction | Zini A.,McGill University | Ravel C.,Rennes University Hospital Center | Belloc S.,Laboratoire dEylau Unilabs | And 6 more authors.
Andrology | Year: 2015

Sperm DNA methylation abnormalities have been detected in oligozoospermic men. However, the association between sperm DNA methylation defects, sperm parameters and sperm DNA, and chromatin integrity remains poorly understood. This study was designed to clarify this issue. We recruited a cohort of 92 men (62 normozoospermic and 30 oligoasthenozoospermic) presenting for infertility evaluation during a 1-year period. Sperm global DNA methylation was evaluated by an ELISA-like method, DNA fragmentation was evaluated by flow cytometry-based terminal transferase dUTP nick end-labeling (TUNEL) assay (reported as DNA fragmentation index or DFI), and sperm denaturation was evaluated by aniline blue staining (reported as sperm denaturation index or SDI, a marker of chromatin compaction). We found a significant positive association between sperm global DNA methylation level and conventional sperm parameters (sperm concentration and motility), supported by the results of methylation analysis on H19-DMR. We also identified significant inverse relationships between sperm global DNA methylation, and, both DFI and SDI. However, sperm global DNA methylation level was not related to sperm vitality or morphology. Our findings suggest that global sperm DNA methylation levels are related to conventional sperm parameters, as well as, sperm chromatin and DNA integrity. © 2015 American Society of Andrology and European Academy of Andrology. Source


Belloc S.,Laboratoire dEylau Unilabs | Benkhalifa M.,Laboratoire dEylau Unilabs | Cohen-Bacrie M.,Laboratoire dEylau Unilabs | Dalleac A.,Laboratoire dEylau Unilabs | And 3 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2014

Background: Sperm DNA damage is common in infertile men and is associated with poor semen parameters but the impact of an isolated sperm abnormality on sperm DNA damage has not been studied. Objective: To evaluate sperm DNA damage in a large cohort of infertile men with isolated sperm defects. Design, setting and participants: Retrospective study of 1084 consecutive, non-azoospermic infertile men with an isolated sperm defect: isolated oligozoospermia (iOligo), isolated asthenozoospermia (iAstheno) or isolated teratozoospermia (iTerato). Outcome measurements and statistical analysis: We examined and compared clinical parameters, conventional semen parameters and %sperm DNA fragmentation (%SDF, assessed by flow cytometry-based Terminal deoxynucleotidyl transferase-mediated dUTP Nick End-Labeling assay) in the three groups of men. Results and limitations: The mean (±SD) %SDF was significantly higher in the iAstheno compared to the iOligo and iTerato groups (25.0±14.0 vs. 19.2±11.6 and 20.7±12.1 %, respectively, P<0.0001). Similarly, the proportion of men with high %SDF (>30 %) was significantly higher in the iAstheno compared to the iOligo and iTerato groups (31 % vs. 18 % and 19 %, respectively, P<0.0001). In the group of 713 men with iAstheno, %SDF was positively correlated with paternal age (r=0.20, P<0.0001) and inversely correlated with %progressive motility (r=-0.18, P<0.0001). In the subset of 218 men with iTerato, %SDF was also positively correlated with paternal age (r=0.15, P=0.018) and inversely correlated with %progressive motility (r=-0.26, P=0.0001). Conclusions: In this large cohort of infertile men with isolated sperm abnormalities, we have found that the sperm DNA fragmentation level is highest in the men with sperm motility defects and that 31 % of these men have high levels of sperm DNA fragmentation. The data indicate that poor motility is the sperm parameter abnormality most closely related to sperm DNA damage. © 2014 Springer Science+Business Media. Source


Prisant N.,Laboratoire dEylau Unilabs | Cohen-Bacrie P.,Laboratoire dEylau Unilabs | Belaisch-Allart J.,Service de gynecologie obstetrique | Cohen-Bacrie M.,Laboratoire dEylau Unilabs | And 3 more authors.
Gynecologie Obstetrique Fertilite | Year: 2011

Objectives: Several studies have reported a time-related decline in semen quality. In this context, 101 404 spermograms-spermocytograms performed in a single andrology laboratory from January 2000 to December 2009 were investigated retrospectively. Variations of sperm parameters were analyzed over the years. Patients and methods: For each semen sample, age at semen collection, duration of sexual abstinence, volume of seminal fluid, pH, concentration of spermatozoa, percentages of overall motile and progressive motile sperm, percentage of morphologically abnormal spermatozoa (according to David's classification) and amplitude of lateral head displacement (ALH) were analyzed. For each parameter, the mean value per year was determined. To examine trends over time the statistical tests used were analyses of variance and correlation studies. Results: Data showed an increasing age of patients from 36.5 years in 2000 to 37.2 years in 2009. The semen concentration was 63.5 millions/mL in 2000 and 63.6 millions/mL in 2009 with a slight fall from 2001 to 2008. The vitality continuously increased from 67.8% in 2000 to 73.5% in 2009 as well as the overall motility (from 37.7% to 39.2%) and the progressive motility (from 34.6% to 36.2%). The percentage of morphologically abnormal spermatozoa continuously increased from 64.5% to 84.2% (r = 0.43, P < 0.001). Discussion and conclusion: Contrary to a variety of works, our very large study of semen analysis did not show a real decline in semen WHO parameters during a 10-year period, except for the percentage of morphologically abnormal spermatozoa, which dramatically increased over the years. © 2011 Elsevier Masson SAS. Source

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