Laboratoire dEvaluation des Materiaux Implantables LEMI

Martillac, France

Laboratoire dEvaluation des Materiaux Implantables LEMI

Martillac, France
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Mathew A.P.,Lulea University of Technology | Oksman K.,Lulea University of Technology | Pierron D.,Laboratoire dEvaluation des Materiaux Implantables LEMI | Harmand M.-F.,Laboratoire dEvaluation des Materiaux Implantables LEMI
Macromolecular Bioscience | Year: 2013

Bio-based fibrous nanocomposites of cellulose nanofibres and non-crosslinked/crosslinked collagen were prepared by in situ pH-induced fibrillation of collagen phase and sterilized using gamma rays at 25 KGy. Collagen phase is crosslinked using genipin, a bio-based crosslinker that introduces flexible crosslinks. Microscopy studies of the prepared materials showed nanostructured fibrous collagen and cellulose dispersed in collagen matrix. Mechanical performance of the sterilized nanocomposites was close to that of natural ligament and tendon, in simulated body conditions. Cytocompatibility studies indicated that these nanocomposites allowed human ligament cell and human endothelial cell adhesion, growth, and differentiation; which is eminently favourable to ligament tissue engineering. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Mathew A.P.,Lulea University of Technology | Oksman K.,Lulea University of Technology | Pierron D.,Laboratoire dEvaluation des Materiaux Implantables LEMI | Harmad M.-F.,Laboratoire dEvaluation des Materiaux Implantables LEMI
Cellulose | Year: 2012

Collagen and cellulose nanofiber based composites were prepared by solution casting followed by pH induced in situ partial fibrillation of collagen phase and crosslinking of collagen phase using gluteraldehyde. Microscopy studies on the materials confirmed the presence of fibrous collagen and cellulose nanofibers embedded in the collagen matrix. The cellulose nanofiber addition as well as collagen crosslinking showed significant positive impact on the nanocomposite's mechanical behaviour. The synergistic performance of the nanocomposites indicated stabilization and reinforcement through strong physical entanglement between collagen and cellulose fibres as well as chemical interaction between collagen matrix and collagen fibrils. The mechanical performance and stability in moist conditions showed the potential of these materials as implantable scaffolds in biomedical applications. The collagen-cellulose ratio, crosslinking agent and crosslinking level of collagen may be further optimised to tailor the mechanical properties and cytocompatibility of these composites for specific applications such as artificial ligament or tendon. © 2011 Springer Science+Business Media B.V.


Mathew A.P.,Sudan University of Science and Technology | Oksman K.,Sudan University of Science and Technology | Pierron D.,Laboratoire dEvaluation des Materiaux Implantables LEMI | Harmand M.-F.,Laboratoire dEvaluation des Materiaux Implantables LEMI
Carbohydrate Polymers | Year: 2012

Fibrous cellulose nanocomposites scaffolds were developed and evaluated for their potential as ligament or tendon substitute. The nanocomposites were prepared by partial dissolution of cellulose nanofiber networks using ionic liquid at 80 °C for different time intervals. Scanning electron microscopy study indicated that partial dissolution resulted in fibrous cellulose nanocomposites where the dissolved cellulose nanofibers formed the matrix phase and the undissolved or partially dissolved nanofibers formed the reinforcing phase. Mechanical properties of the composites in simulated body conditions (37 °C and 95% RH) after sterilization using gamma rays was comparable to those of natural ligaments and tendons. Stress relaxation studies showed stable performance towards cyclic loading and unloading, further confirming the possibility for using these composites as ligament/tendon substitute. In vitro biocompatibility showed a positive response concerning adhesion/proliferation and differentiation for both human ligament and endothelial cells. Prototypes based on the cellulose composite were developed in the form of tubules to be used for further studies. © 2011 Elsevier Ltd. All rights reserved.

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