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Couderc M.,CNRS Sea, Molecules and Health | Marchand J.,CNRS Sea, Molecules and Health | Zalouk-Vergnoux A.,CNRS Sea, Molecules and Health | Kamari A.,CNRS Sea, Molecules and Health | And 5 more authors.
Science of the Total Environment | Year: 2016

In teleost fish, thyroid function is involved in various critical physiological processes. Given the complexity of the hypothalamo-pituitary-thyroid (HPT) axis, a large number of genes and proteins can be the potential target of endocrine-disrupting compounds (EDCs). The aim of this study was to evaluate, in yellow and silver European eels (Anguilla anguilla), potential effects of EDCs on thyroid status by analyzing the associations between EDC body burdens and thyroid hormones (THs). In yellow individuals, greater free T3/T4 ratios (FT3/FT4) and lower plasma FT4 levels were associated with greater concentrations of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), as highlighted by significant correlations with many congeners. Few positive relationships with alkylphenols were noticed. In contrast, silver eels usually exhibited less significant correlations between THs and contaminant loads. Expression of a series of genes involved in the HPT axis was also investigated in the silver individuals. Concerning mRNA expression in silver females, some main correlations were noticed: thyroid-stimulating hormone (TSHβ) gene expression was significantly correlated to numerous PCBs, and hepatic mRNA levels of deiodinase 2 (Deio 2) were negatively correlated to 2-hydroxyfluorene (2-OHF) and 2-naphtol (2-OHNa). Thyroid receptor (TRα and TRβ) mRNA levels exhibited weak negative correlations with some PBDEs in silver females and males. Hepatic vitellogenin (Vtg) mRNA levels were detected in all silver males but at lower levels than in silver females. In males, Vtg mRNA levels were positively associated to FT4/TT4. In silver females, strong positive correlations were found between congeners of PCBs, PBDEs and PFAS suggesting potential estrogenic effects. Overall, the observed results indicate that several organic contaminants, mainly dl-, ndl-PCBs and PBDEs, could be associated with changes in thyroid homeostasis in these fish, via direct or indirect interactions with peripheral deiodination, metabolism of T4 and mechanisms involved in TSHβ, Deio 2 and Vtg gene transcription. © 2016 Elsevier B.V.

Nunez O.,University of Barcelona | Gallart-Ayala H.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Martins C.P.B.,Thermo Fisher Scientific | Lucci P.,Pontifical Xavierian University | Busquets R.,University of Brighton
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

There is an increasing need of new bio-analytical methodologies with enough sensitivity, robustness and resolution to cope with the analysis of a large number of analytes in complex matrices in short analysis time. For this purpose, all steps included in any bio-analytical method (sampling, extraction, clean-up, chromatographic analysis and detection) must be taken into account to achieve good and reliable results with cost-effective methodologies. The purpose of this review is to describe the state-of-the-art of the most employed technologies in the period 2009-2012 to achieve fast analysis with liquid chromatography coupled to mass spectrometry (LC-MS) methodologies for bio-analytical applications. Current trends in fast liquid chromatography involve the use of several column technologies and this review will focus on the two most frequently applied: sub-2. μm particle size packed columns to achieve ultra high pressure liquid chromatography (UHPLC) separations and porous-shell particle packed columns to attain high efficiency separations with reduced column back-pressures. Additionally, recent automated sample extraction and clean-up methodologies to reduce sample manipulation, variability and total analysis time in bio-analytical applications such as on-line solid phase extraction coupled to HPLC or UHPLC methods, or the use of other approaches such as molecularly imprinted polymers, restricted access materials, and turbulent flow chromatography will also be addressed. The use of mass spectrometry and high or even ultra-high resolution mass spectrometry to reduce sample manipulation and to solve ion suppression or ion enhancement and matrix effects will also be presented. The advantages and drawbacks of all these methodologies for fast and sensitive analysis of biological samples are going to be discussed by means of relevant applications. © 2013 Elsevier B.V..

Alexandre-Gouabau M.-C.,Nantes University Hospital Center | Alexandre-Gouabau M.-C.,French National Institute for Agricultural Research | Courant F.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Le Gall G.,UK Institute of Food Research | And 5 more authors.
Journal of Proteome Research | Year: 2011

Intrauterine growth restriction (IUGR), along with postnatal growth trajectory, is closely linked with metabolic diseases and obesity at adulthood. The present study reports the time-dependent metabolomic response of male offspring of rat dams exposed to maternal adequate protein diet during pregnancy and lactation (CC) or protein deprivation during pregnancy only (IUGR with rapid catch-up growth, RC) or through pregnancy and lactation (IUGR with slow postnatal growth, RR). Plasma LC-HRMS metabolomic fingerprints for 8 male rats per group, combined with multivariate statistical analysis (PLS-DA and HCA), were used to study the impact of IUGR and postnatal growth velocity on the offspring metabolism in early life (until weaning) and once they reached adulthood (8 months). Compared with CC rats, RR pups had clear-cut alterations in plasma metabolome during suckling, but none at adulthood; in contrast, in RC pups, alterations in metabolome were minimal in early life but more pronounced in the long run. In particular, our results pinpoint transient alterations in proline, arginine, and histidine in RR rats, compared to CC rats, and persistent differences in tyrosine and carnitine, compared to RC rats at adulthood. These findings suggest that the long-term deregulation in feeding behavior and fatty acid metabolism in IUGR rats depends on postnatal growth velocity. © 2011 American Chemical Society.

Ounnas F.,University of Lorraine | Feidt C.,University of Lorraine | Toussaint H.,University of Lorraine | Marchand P.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | And 3 more authors.
Environmental Science and Technology | Year: 2010

This study investigated milk excretion kinetics of PCBs, tetraBDE (BDE 47), and penta-BDE (BDE 99) in goats exposed to contaminated soil under controlled conditions. The animals were fed (80 days) with feed containing 5% of contaminated soil. During this exposure period, milk was analyzed weekly. At the end of the experiment the PCBs and PBDEs retained in hepatic and adipose tissues were also determined. The soilmilk carry over rates (CORs) of PCBs ranged from 6 to 62%. This result suggests that a large part of ingested soil-bound PCBs was recovered in milk. Significantly different levels between the congeners were reported in the tissues (fat, liver). BDE 47 and 99 excretions in milk achieved a plateau after 2 weeks of exposure, and their corresponding CORs were about 30%. These two congeners showed a significantly (P < 0.05) lower accumulation in the adipose tissue than the major PCB congeners. The concentrations of BDE 47 and 99 in the liver were the same as PCB concentrations. This result suggests that the low brominated congeners are submitted to the metabolism more extensively than the major PCBs. © 2010 American Chemical Society.

Deceuninck Y.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Bichon E.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Silcock P.,Waters Corporation | Rontree S.,Waters Corporation | And 3 more authors.
Food Science and Technology | Year: 2011

Yoann Deceuninck and his associates investigate the high sensitivity detection of the two dexamethasone and betamethasone growth promoters in liver and milk. Dexamethasone and betamethasone are synthetic glucocorticoids that are widely used in animal husbandry. These epimeric compounds are licensed for therapy in veterinary practice while their use as growth promoters is banned within the European Union. The European Community has fixed Maximum Residue Limits (MRLs) for the two molecules in several matrices to protect consumer's safety. The major challenge in the analysis of dexamethasone and betamethasone is in performing an efficient separation of both isomers and in detecting and identifying these molecules at the suitable MRL.

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