Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA

Nantes, France

Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA

Nantes, France
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Alexandre-Gouabau M.-C.,Nantes University Hospital Center | Alexandre-Gouabau M.-C.,French National Institute for Agricultural Research | Courant F.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Le Gall G.,UK Institute of Food Research | And 5 more authors.
Journal of Proteome Research | Year: 2011

Intrauterine growth restriction (IUGR), along with postnatal growth trajectory, is closely linked with metabolic diseases and obesity at adulthood. The present study reports the time-dependent metabolomic response of male offspring of rat dams exposed to maternal adequate protein diet during pregnancy and lactation (CC) or protein deprivation during pregnancy only (IUGR with rapid catch-up growth, RC) or through pregnancy and lactation (IUGR with slow postnatal growth, RR). Plasma LC-HRMS metabolomic fingerprints for 8 male rats per group, combined with multivariate statistical analysis (PLS-DA and HCA), were used to study the impact of IUGR and postnatal growth velocity on the offspring metabolism in early life (until weaning) and once they reached adulthood (8 months). Compared with CC rats, RR pups had clear-cut alterations in plasma metabolome during suckling, but none at adulthood; in contrast, in RC pups, alterations in metabolome were minimal in early life but more pronounced in the long run. In particular, our results pinpoint transient alterations in proline, arginine, and histidine in RR rats, compared to CC rats, and persistent differences in tyrosine and carnitine, compared to RC rats at adulthood. These findings suggest that the long-term deregulation in feeding behavior and fatty acid metabolism in IUGR rats depends on postnatal growth velocity. © 2011 American Chemical Society.


Cesbron N.,Laboratoire Detude Des Residus Et Contaminants Dans Les Aliments Laberca | Royer A.-L.,Laboratoire Detude Des Residus Et Contaminants Dans Les Aliments Laberca | Guitton Y.,Laboratoire Detude Des Residus Et Contaminants Dans Les Aliments Laberca | Sydor A.,Laboratoire Detude Des Residus Et Contaminants Dans Les Aliments Laberca | And 2 more authors.
Metabolomics | Year: 2017

Introduction: Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. Objectives: In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. Methods: The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. Results: A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. Conclusion: The workflow generated repeatable and informative fingerprints for robust metabolome characterization. © 2017, Springer Science+Business Media, LLC.


Nunez O.,University of Barcelona | Gallart-Ayala H.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Martins C.P.B.,Thermo Fisher Scientific | Lucci P.,Pontifical Xavierian University | Busquets R.,University of Brighton
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

There is an increasing need of new bio-analytical methodologies with enough sensitivity, robustness and resolution to cope with the analysis of a large number of analytes in complex matrices in short analysis time. For this purpose, all steps included in any bio-analytical method (sampling, extraction, clean-up, chromatographic analysis and detection) must be taken into account to achieve good and reliable results with cost-effective methodologies. The purpose of this review is to describe the state-of-the-art of the most employed technologies in the period 2009-2012 to achieve fast analysis with liquid chromatography coupled to mass spectrometry (LC-MS) methodologies for bio-analytical applications. Current trends in fast liquid chromatography involve the use of several column technologies and this review will focus on the two most frequently applied: sub-2. μm particle size packed columns to achieve ultra high pressure liquid chromatography (UHPLC) separations and porous-shell particle packed columns to attain high efficiency separations with reduced column back-pressures. Additionally, recent automated sample extraction and clean-up methodologies to reduce sample manipulation, variability and total analysis time in bio-analytical applications such as on-line solid phase extraction coupled to HPLC or UHPLC methods, or the use of other approaches such as molecularly imprinted polymers, restricted access materials, and turbulent flow chromatography will also be addressed. The use of mass spectrometry and high or even ultra-high resolution mass spectrometry to reduce sample manipulation and to solve ion suppression or ion enhancement and matrix effects will also be presented. The advantages and drawbacks of all these methodologies for fast and sensitive analysis of biological samples are going to be discussed by means of relevant applications. © 2013 Elsevier B.V..


Ounnas F.,University of Lorraine | Feidt C.,University of Lorraine | Toussaint H.,University of Lorraine | Marchand P.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | And 3 more authors.
Environmental Science and Technology | Year: 2010

This study investigated milk excretion kinetics of PCBs, tetraBDE (BDE 47), and penta-BDE (BDE 99) in goats exposed to contaminated soil under controlled conditions. The animals were fed (80 days) with feed containing 5% of contaminated soil. During this exposure period, milk was analyzed weekly. At the end of the experiment the PCBs and PBDEs retained in hepatic and adipose tissues were also determined. The soilmilk carry over rates (CORs) of PCBs ranged from 6 to 62%. This result suggests that a large part of ingested soil-bound PCBs was recovered in milk. Significantly different levels between the congeners were reported in the tissues (fat, liver). BDE 47 and 99 excretions in milk achieved a plateau after 2 weeks of exposure, and their corresponding CORs were about 30%. These two congeners showed a significantly (P < 0.05) lower accumulation in the adipose tissue than the major PCB congeners. The concentrations of BDE 47 and 99 in the liver were the same as PCB concentrations. This result suggests that the low brominated congeners are submitted to the metabolism more extensively than the major PCBs. © 2010 American Chemical Society.


Deceuninck Y.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Bichon E.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Silcock P.,Waters Corporation | Rontree S.,Waters Corporation | And 3 more authors.
Food Science and Technology | Year: 2011

Yoann Deceuninck and his associates investigate the high sensitivity detection of the two dexamethasone and betamethasone growth promoters in liver and milk. Dexamethasone and betamethasone are synthetic glucocorticoids that are widely used in animal husbandry. These epimeric compounds are licensed for therapy in veterinary practice while their use as growth promoters is banned within the European Union. The European Community has fixed Maximum Residue Limits (MRLs) for the two molecules in several matrices to protect consumer's safety. The major challenge in the analysis of dexamethasone and betamethasone is in performing an efficient separation of both isomers and in detecting and identifying these molecules at the suitable MRL.


Couderc M.,CNRS Sea, Molecules and Health | Marchand J.,CNRS Sea, Molecules and Health | Zalouk-Vergnoux A.,CNRS Sea, Molecules and Health | Kamari A.,CNRS Sea, Molecules and Health | And 5 more authors.
Science of the Total Environment | Year: 2016

In teleost fish, thyroid function is involved in various critical physiological processes. Given the complexity of the hypothalamo-pituitary-thyroid (HPT) axis, a large number of genes and proteins can be the potential target of endocrine-disrupting compounds (EDCs). The aim of this study was to evaluate, in yellow and silver European eels (Anguilla anguilla), potential effects of EDCs on thyroid status by analyzing the associations between EDC body burdens and thyroid hormones (THs). In yellow individuals, greater free T3/T4 ratios (FT3/FT4) and lower plasma FT4 levels were associated with greater concentrations of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), as highlighted by significant correlations with many congeners. Few positive relationships with alkylphenols were noticed. In contrast, silver eels usually exhibited less significant correlations between THs and contaminant loads. Expression of a series of genes involved in the HPT axis was also investigated in the silver individuals. Concerning mRNA expression in silver females, some main correlations were noticed: thyroid-stimulating hormone (TSHβ) gene expression was significantly correlated to numerous PCBs, and hepatic mRNA levels of deiodinase 2 (Deio 2) were negatively correlated to 2-hydroxyfluorene (2-OHF) and 2-naphtol (2-OHNa). Thyroid receptor (TRα and TRβ) mRNA levels exhibited weak negative correlations with some PBDEs in silver females and males. Hepatic vitellogenin (Vtg) mRNA levels were detected in all silver males but at lower levels than in silver females. In males, Vtg mRNA levels were positively associated to FT4/TT4. In silver females, strong positive correlations were found between congeners of PCBs, PBDEs and PFAS suggesting potential estrogenic effects. Overall, the observed results indicate that several organic contaminants, mainly dl-, ndl-PCBs and PBDEs, could be associated with changes in thyroid homeostasis in these fish, via direct or indirect interactions with peripheral deiodination, metabolism of T4 and mechanisms involved in TSHβ, Deio 2 and Vtg gene transcription. © 2016 Elsevier B.V.


Deceuninck Y.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Bichon E.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Monteau F.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Antignac J.-P.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA | Le Bizec B.,Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA
Analytica Chimica Acta | Year: 2011

Dexamethasone, betamethasone, prednisolone and methylprednisolone are corticosteroids widely used in animal husbandry. These compounds are licensed for therapy in veterinary practices while their use for growth promoting practices, mainly in combination with other growth promoters, is prohibited within the European Union. In order to protect the consumer, maximum residue limits (MRLs) have been set by the European Community in liver to 2.0μgkg-1 (dexamethasone and betamethasone) and 10.0μgkg-1 (prednisolone and methylprednisolone) for different species. The major challenges in the analysis of dexamethasone and betamethasone consist in performing an efficient separation of both isomers and in detecting and identifying all the molecules according to the regulatory requirements fixed in Commission Decision 2002/657/EC. In this context, an UHPLC-MS/MS method with a short runtime (7min) and using the SRM acquisition mode was developed and validated. An efficient selectivity of the sample preparation combined with a high sensitivity of the measurement system allowed identifying and quantifying the four corticosteroids of interest in this complex biological matrix. Signals obtained were found very repeatable, even at very low concentration levels with an unambiguous identification of the compounds. The performance limits of the method have been validated according to the regulatory requirements and the method has been successfully applied to the confirmation of incurred liver samples. © 2011 Elsevier B.V.


PubMed | Catholic University of the West, Laboratoire dEtude des Residus et Contaminants dans les Aliments LABERCA and CNRS Sea, Molecules and Health
Type: | Journal: The Science of the total environment | Year: 2016

In teleost fish, thyroid function is involved in various critical physiological processes. Given the complexity of the hypothalamo-pituitary-thyroid (HPT) axis, a large number of genes and proteins can be the potential target of endocrine-disrupting compounds (EDCs). The aim of this study was to evaluate, in yellow and silver European eels (Anguilla anguilla), potential effects of EDCs on thyroid status by analyzing the associations between EDC body burdens and thyroid hormones (THs). In yellow individuals, greater free T3/T4 ratios (FT3/FT4) and lower plasma FT4 levels were associated with greater concentrations of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), as highlighted by significant correlations with many congeners. Few positive relationships with alkylphenols were noticed. In contrast, silver eels usually exhibited less significant correlations between THs and contaminant loads. Expression of a series of genes involved in the HPT axis was also investigated in the silver individuals. Concerning mRNA expression in silver females, some main correlations were noticed: thyroid-stimulating hormone (TSH) gene expression was significantly correlated to numerous PCBs, and hepatic mRNA levels of deiodinase 2 (Deio 2) were negatively correlated to 2-hydroxyfluorene (2-OHF) and 2-naphtol (2-OHNa). Thyroid receptor (TR and TR) mRNA levels exhibited weak negative correlations with some PBDEs in silver females and males. Hepatic vitellogenin (Vtg) mRNA levels were detected in all silver males but at lower levels than in silver females. In males, Vtg mRNA levels were positively associated to FT4/TT4. In silver females, strong positive correlations were found between congeners of PCBs, PBDEs and PFAS suggesting potential estrogenic effects. Overall, the observed results indicate that several organic contaminants, mainly dl-, ndl-PCBs and PBDEs, could be associated with changes in thyroid homeostasis in these fish, via direct or indirect interactions with peripheral deiodination, metabolism of T4 and mechanisms involved in TSH, Deio 2 and Vtg gene transcription.

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