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Saint-André-lez-Lille, France

Pelfrene A.,University of Lille Nord de France | Pelfrene A.,ISA Group | Waterlot C.,University of Lille Nord de France | Waterlot C.,ISA Group | And 13 more authors.
Environmental Pollution | Year: 2012

An investigation was undertaken to identify the most significant soil parameters that can be used to predict Cd, Pb, and Zn bioaccessibility in smelter-contaminated agricultural soils. A robust model was established from an extended database of soils by using: (i) a training set of 280 samples to select the main soil parameters, to define the best population to be taken into account for the model elaboration, and to construct multivariate regression models, and (ii) a test set of 110 samples to validate the ability of the regression models. Total carbonate, organic matter, sand, P2O 5, free Fe-Mn oxide, and pseudototal Al and trace element (TE) contents appeared as the main variables governing TE bioaccessibility. The statistical modeling approach was reasonably successful, indicating that the main soil factors influencing the bioaccessibility of TEs were taken into account and the predictions could be applicable for further risk evaluation in the studied area. © 2011 Elsevier Ltd. All rights reserved.

Canivet L.,Laboratoire des science Vegetales et Fongiques | Dubot P.,CNRS East Paris Institute of Chemistry and Materials Science | Garcon G.,Faculte des science Pharmaceutiques et Biologiques | Denayer F.-O.,Laboratoire des science Vegetales et Fongiques
Ecotoxicology and Environmental Safety | Year: 2015

The effects of iron nanoparticles on bryophytes (. Physcomitrella patens) were studied following foliar exposure. We used iron nanoparticles (Fe-NP) representative of industrial emissions from the metallurgical industries. After a characterization of iron nanoparticles and the validation of nanoparticle internalization in cells, the effects (cytotoxicity, oxidative stress, lipid peroxidation of membrane) of iron nanoparticles were determined through the axenic culturing of Physcomitrella patens exposed at five different concentrations (5. ng, 50. ng, 500. ng, 5. μg and 50. μg per plant). Following exposure, the plant health, measured as ATP concentrations, was not impacted. Moreover, we studied oxidative stress in three ways: through the measure of reactive oxygen species (ROS) production, through malondialdehyde (MDA) production and also through glutathione regulation. At concentrations tested over a short period, the level of ROS, MDA and glutathione were not significantly disturbed. © 2015.

Canivet L.,Laboratoire des science Vegetales et Fongiques | Dubot P.,CNRS East Paris Institute of Chemistry and Materials Science | Denayer F.-O.,Laboratoire des science Vegetales et Fongiques
Journal of Bryology | Year: 2014

Iron nanoparticles were produced in a radiofrequency levitation furnace and their dimensions and purity determined by scanning electron microscopy and x-ray microanalysis. Confocal microscopy revealed that these particles penetrated the leaves of Aphanorrhegma when applied to the plants as mineral water suspensions. This, the first demonstration of nanoparticle uptake by a bryophyte, opens the way to nanotoxicological studies paralleling those in higher plants. The actual penetration mechanism in both groups remains unknown. © British Bryological Society 2014.

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